Information on EC 3.2.1.62 - glycosylceramidase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY
3.2.1.62
-
RECOMMENDED NAME
GeneOntology No.
glycosylceramidase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
glycosyl-N-acylsphingosine + H2O = a ceramide + a sugar
show the reaction diagram
mechanism
-
glycosyl-N-acylsphingosine + H2O = a ceramide + a sugar
show the reaction diagram
; broad specificity; broad specificity, cf. EC 3.2.1.45, glucosylceramidase, and EC 3.2.1.46, galactosylceramidase. Also hydrolyses phlorizin to phloretin and glucose. The intestinal enzyme is a complex that also catalyses the reaction of EC 3.2.1.108, lactase
-
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
hydrolysis of O-glycosyl bond
-
-
-
-
hydrolysis of O-glycosyl bond
-
-
hydrolysis of O-glycosyl bond
-
-
hydrolysis of O-glycosyl bond
-
-
hydrolysis of O-glycosyl bond
-
-
hydrolysis of O-glycosyl bond
-
-
hydrolysis of O-glycosyl bond
Rhodococcus sp. M-777
-
-
-
SYSTEMATIC NAME
IUBMB Comments
glycosyl-N-acylsphingosine glycohydrolase
Broad specificity [cf. EC 3.2.1.45 (glucosylceramidase) and EC 3.2.1.46 (galactosylceramidase)]. Also hydrolyses phlorizin to phloretin and glucose. The intestinal enzyme is a complex that also catalyses the reaction of EC 3.2.1.108 lactase.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
cerebrosidase
-
-
-
-
EGC
Rhodococcus sp. M-777
-
-
-
endo-glycoceramidase II
-
membrane-associated family 5 glycosidase
endo-glycoceramidase II
Rhodococcus sp. M-777
-
membrane-associated family 5 glycosidase
-
glycosyl ceramide glycosylhydrolase
-
-
-
-
glycosyl-N-acylsphingosine glycohydrolase
-
-
glycosyl-N-acylsphingosine glycohydrolase
-
-
glycosyl-N-acylsphingosine glycohydrolase
-
-
glycosyl-N-acylsphingosine glycohydrolase
-
-
glycosylceramidase
-
-
glycosylceramidase
-
-
glycosylceramidase
-
-
glycosylceramidase
-
-
glycosylceramidase
-
-
glycosylceramidase
Rhodococcus sp. M-777
-
-
-
glycosylceramidase
-
-
KLotho lactase phlorizin hydrolase
-
KLPH
Klotho-related protein
-
KlrP, novel cytosolic neutral glycosylceramidase
lactase phlorizin hydrolase
-
-
lactase phlorizin hydrolase
-
-
lactase phlorizin hydrolase
-
-
lactase-phlorizin hydrolase
-
-
-
-
lactase-phlorizin hydrolase
-
-
lactase-phlorizin hydrolase
-
brush border membrane (BBM)-associated enzyme in intestinal cells
lactase-phlorizin hydrolase
-
membrane disaccharidase
lactase-phlorizin hydrolase
-
-
LPH
-
-
phloretin-glucosidase
-
-
-
-
phloridzin glucosidase
-
-
-
-
phlorizin beta-glucosidase
-
-
-
-
phlorizin hydrolase
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY
9033-10-7
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
lamb, bifunctional lactase/phlorizin hydrolase complex
-
-
Manually annotated by BRENDA team
Ovis aries aries
sheep
-
-
Manually annotated by BRENDA team
male sprague-dawley rats
-
-
Manually annotated by BRENDA team
strain M-777
-
-
Manually annotated by BRENDA team
Rhodococcus sp. M-777
strain M-777
-
-
Manually annotated by BRENDA team
food supplemented with insulin-like growth factor I
-
-
Manually annotated by BRENDA team
puffer fish, no activity in Mycetozoa, slime mold
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
physiological function
-
The profragment, LPHalpha, comprises homologous domains I and II and functions as an intramolecular chaperone in the context of the brush-border LPHbeta region of LPH
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(2-hydroxymethylphenyl)-beta-D-glucopyranoside + H2O
2-hydroxymethylphenol + beta-D-glucose
show the reaction diagram
-
low activity, salicin
-
?
2-naphthyl-beta-D-galactopyranoside + H2O
naphthol + beta-D-galactopyranose
show the reaction diagram
-
very low activity
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
show the reaction diagram
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
show the reaction diagram
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
show the reaction diagram
-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
show the reaction diagram
-
lower activity than for 4-nitrophenyl-beta-D-galactopyranoside
-
?
2-nitrophenyl beta-D-glucopyranoside + H2O
2-nitrophenol + beta-D-glucose
show the reaction diagram
-
-
-
?
3'-fucosyllactose + H2O
beta-D-glucose + 3-O-(6-deoxy-alpha-L-galactopyranosyl)-beta-D-galactopyranose
show the reaction diagram
-
only oligosaccharides containing galactose as the glycone in beta-linkage hydrolysed
-
?
4'-deoxyphlorizin + H2O
4'-deoxyphloritin + beta-D-glucose
show the reaction diagram
-
low activity
-
?
4-methylumbelliferyl-alpha-L-arabinoside + H2O
4-methylumbelliferone + alpha-L-arabinose
show the reaction diagram
-
-
-
?
4-methylumbelliferyl-beta-D-galactopyranoside + H2O
4-methylumbelliferyl + D-galactose
show the reaction diagram
-
-
-
-
?
4-methylumbelliferyl-beta-D-galactoside + H2O
4-methylumbelliferone + beta-D-galactose
show the reaction diagram
-
-
-
?
4-methylumbelliferyl-beta-D-galactoside + H2O
4-methylumbelliferone + beta-D-galactose
show the reaction diagram
-
-
-
?
4-methylumbelliferyl-beta-D-glucopyranoside + H2O
4-methylumbelliferyl + D-glucose
show the reaction diagram
-
-
-
-
?
4-methylumbelliferyl-beta-D-glucoside + H2O
4-methylumbelliferone + beta-D-glucose
show the reaction diagram
-
-
-
?
4-methylumbelliferyl-beta-D-xyloside + H2O
4-methylumbelliferone + beta-D-xylose
show the reaction diagram
-
low activity
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + beta-D-glucose
show the reaction diagram
-
-
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + beta-D-glucose
show the reaction diagram
-
-
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + beta-D-glucose
show the reaction diagram
-
-
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + beta-D-glucose
show the reaction diagram
-
-
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + beta-D-glucose
show the reaction diagram
-
-
-
?
4-nitrophenyl-beta-D-fucoside + H2O
4-nitrophenol + beta-D-fucose
show the reaction diagram
-
-
-
?
4-nitrophenyl-beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactose
show the reaction diagram
-
-
-
?
4-nitrophenyl-beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactose
show the reaction diagram
-
-
-
?
4-nitrophenyl-beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactose
show the reaction diagram
-
-
-
?
4-nitrophenyl-beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactose
show the reaction diagram
-
low activity
-
?
4-O-beta-D-galactopyranosyl-D-fructoside + H2O
beta-D-galactose + D-fructose
show the reaction diagram
-
very low activity
-
?
4-O-beta-D-galactopyranosyl-D-glucopyranoside + H2O
D-galactose + beta-D-glucose
show the reaction diagram
-
-
-
?
4-O-beta-D-galactopyranosyl-D-glucopyranoside + H2O
D-galactose + beta-D-glucose
show the reaction diagram
-
-
-
?
4-O-beta-D-galactopyranosyl-D-glucopyranoside + H2O
D-galactose + beta-D-glucose
show the reaction diagram
-
-
-
?
4-O-beta-D-galactopyranosyl-D-glucopyranoside + H2O
D-galactose + beta-D-glucose
show the reaction diagram
-
-
-
?
4-O-beta-D-galactopyranosyl-D-glucopyranoside + H2O
D-galactose + beta-D-glucose
show the reaction diagram
-
-
-
?
4-O-beta-D-galactopyranosyl-D-glucopyranoside + H2O
D-galactose + beta-D-glucose
show the reaction diagram
-
-
-
?
4-O-beta-D-galactopyranosyl-D-glucopyranoside + H2O
D-galactose + beta-D-glucose
show the reaction diagram
-
-
-
?
4-O-beta-D-galactopyranosyl-D-glucopyranoside + H2O
D-galactose + beta-D-glucose
show the reaction diagram
-
-
-
?
4-O-beta-D-galactopyranosyl-D-glucopyranoside + H2O
D-galactose + beta-D-glucose
show the reaction diagram
-
best substrate
-
?
4-O-beta-D-galactopyranosyl-D-glucopyranoside + H2O
D-galactose + beta-D-glucose
show the reaction diagram
-
best substrate
-
?
4-O-beta-D-galactopyranosyl-D-glucopyranoside + H2O
D-galactose + beta-D-glucose
show the reaction diagram
-
best substrate
-
?
4-O-beta-D-galactopyranosyl-D-glucopyranoside + H2O
D-galactose + beta-D-glucose
show the reaction diagram
-
best substrate
-
?
4-O-beta-D-galactopyranosyl-D-glucopyranoside + H2O
D-galactose + beta-D-glucose
show the reaction diagram
-
best substrate
-
?
4-O-beta-D-galactopyranosyl-D-glucopyranoside + H2O
D-galactose + beta-D-glucose
show the reaction diagram
-
hydrolysis located on a specific active site for lactose hydrolysis
-
?
4-O-beta-D-galactopyranosyl-D-glucopyranoside + H2O
D-galactose + beta-D-glucose
show the reaction diagram
Ovis aries aries
-
hydrolysis located on a specific active site for lactose hydrolysis
-
?
4-O-beta-D-galactopyranosyl-D-glucopyranoside + H2O
D-galactose + beta-D-glucose
show the reaction diagram
-
two enzymatic activites, one for lactose- and one for phlorizin-and lactose-hydrolysis
-
?
4-O-beta-D-galactopyranosyl-D-glucopyranoside + H2O
D-galactose + beta-D-glucose
show the reaction diagram
-
two enzymatic activites, one for lactose- and one for phlorizin-and lactose-hydrolysis
-
?
4-O-beta-D-galactopyranosyl-D-glucopyranoside + H2O
D-galactose + beta-D-glucose
show the reaction diagram
-
two enzymatic activites, one for lactose- and one for phlorizin-and lactose-hydrolysis
-
?
4-O-beta-D-galactopyranosyl-D-glucopyranoside + H2O
D-galactose + beta-D-glucose
show the reaction diagram
-
two enzymatic activites, one for lactose- and one for phlorizin-and lactose-hydrolysis
-
?
4-O-beta-D-galactopyranosyl-D-glucopyranoside + H2O
D-galactose + beta-D-glucose
show the reaction diagram
-
two enzymatic activites, one for lactose- and one for phlorizin-and lactose-hydrolysis
-
?
4-O-beta-D-galactopyranosyl-D-glucopyranoside + H2O
D-galactose + beta-D-glucose
show the reaction diagram
-
two enzymatic activites, one for lactose- and one for phlorizin-and lactose-hydrolysis
-
?
4-O-beta-D-galactopyranosyl-D-glucopyranoside + H2O
D-galactose + beta-D-glucose
show the reaction diagram
-
4fold higher activity for the N- and O-glycosylated than for the N-glycosylated molecule
-
?
4-O-beta-D-galactopyranosyl-D-glucopyranoside + H2O
D-galactose + beta-D-glucose
show the reaction diagram
-
acts on dietary glycosides before absorption
-
?
4-O-beta-D-galactopyranosyl-D-glucopyranoside + H2O
D-galactose + beta-D-glucose
show the reaction diagram
Ovis aries aries
-
acts on dietary glycosides before absorption
-
?
4-O-beta-D-galactopyranosyl-D-glucopyranoside + H2O
D-galactose + beta-D-glucose
show the reaction diagram
-
two enzymatic activities, one for lactose- and one for phlorizin-and lactose-hydrolysis
-
?
4-O-beta-D-galactopyranosyl-D-glucopyranoside + H2O
D-galactose + beta-D-glucose
show the reaction diagram
-
two enzymatic activities, one for lactose- and one for phlorizin-and lactose-hydrolysis
-
?
4-O-beta-D-galactopyranosyl-D-glucopyranoside + H2O
D-galactose + beta-D-glucose
show the reaction diagram
-
two enzymatic activities, one for lactose- and one for phlorizin-and lactose-hydrolysis
-
?
4-O-beta-D-galactopyranosyl-D-glucopyranoside + H2O
D-galactose + beta-D-glucose
show the reaction diagram
-
two enzymatic activities, one for lactose- and one for phlorizin-and lactose-hydrolysis
-
?
4-O-beta-D-galactopyranosyl-D-glucopyranoside + H2O
D-galactose + beta-D-glucose
show the reaction diagram
-
two enzymatic activities, one for lactose- and one for phlorizin-and lactose-hydrolysis
-
?
4-O-beta-D-galactopyranosyl-D-glucopyranoside + H2O
D-galactose + beta-D-glucose
show the reaction diagram
-
two enzymatic activities, one for lactose- and one for phlorizin-and lactose-hydrolysis
-
?
4-O-beta-D-galactopyranosyl-D-N-acetylglucosamine + H2O
beta-D-galactose + N-acetylglucosamine
show the reaction diagram
-
poor activity
-
?
4-O-beta-D-glucopyranosyl-D-glucose + H2O
beta-D-glucose + D-glucose
show the reaction diagram
-
cellobiose
-
?
4-O-beta-D-glucopyranosyl-D-glucose + H2O
beta-D-glucose + D-glucose
show the reaction diagram
-
cellobiose
-
?
4-O-beta-D-glucopyranosyl-D-glucose + H2O
beta-D-glucose + D-glucose
show the reaction diagram
-
cellobiose
-
?
4-O-beta-D-glucopyranosyl-D-glucose + H2O
beta-D-glucose + D-glucose
show the reaction diagram
-
cellobiose
-
?
4-O-beta-D-glucopyranosyl-D-glucose + H2O
beta-D-glucose + D-glucose
show the reaction diagram
-
cellobiose
-
?
4-O-beta-D-glucopyranosyl-D-glucose + H2O
beta-D-glucose + D-glucose
show the reaction diagram
-
cellobiose
-
?
4-O-beta-D-glucopyranosyl-D-glucose + H2O
beta-D-glucose + D-glucose
show the reaction diagram
-
cellobiose
-
?
6-bromonaphthyl-beta-D-galactopyranoside
6-bromonaphthol + beta-D-galactopyranose
show the reaction diagram
-
very low activity
-
?
6-O-beta-D-glucopyranosyl-D-glucose + H2O
beta-D-glucose + D-glucose
show the reaction diagram
-
low activity
-
?
6-O-beta-D-glucopyranosyl-D-glucose + H2O
beta-D-glucose + D-glucose
show the reaction diagram
-
low activity
-
?
6-O-beta-D-glucopyranosyl-D-glucose + H2O
beta-D-glucose + D-glucose
show the reaction diagram
-
gentiobiose
-
?
6-O-beta-D-glucopyranosyl-D-glucose + H2O
beta-D-glucose + D-glucose
show the reaction diagram
-
gentiobiose
-
?
6-O-beta-D-glucopyranosyl-D-glucose + H2O
beta-D-glucose + D-glucose
show the reaction diagram
-
gentiobiose
-
?
beta-methylglucoside + H2O
methanol + beta-D-glucose
show the reaction diagram
-
-
-
?
C18-galactosylceramide + H2O
C18-ceramide + D-galactose
show the reaction diagram
-
-
-
-
?
C18-glucosylceramide + H2O
C18-ceramide + D-glucose
show the reaction diagram
-
-
-
-
?
C6-4-nitrobenzo-2-oxa-1,3-diazole-galactosylceramide + H2O
C6-4-nitrobenzo-2-oxa-1,3-diazole-ceramide + D-galactose
show the reaction diagram
-
-
-
-
?
C6-4-nitrobenzo-2-oxa-1,3-diazole-glucosylceramide + H2O
C6-4-nitrobenzo-2-oxa-1,3-diazole-ceramide + D-glucose
show the reaction diagram
-
C6-NBD-glucosylceramide
-
-
?
cellotetraose + H2O
? + beta-D-glucose
show the reaction diagram
-
considerable activity
-
?
cellotriose + H2O
? + beta-D-glucose
show the reaction diagram
-
considerable activity
-
?
cellulose + H2O
? + beta-D-glucose
show the reaction diagram
-
very low activity
-
?
D-erythro-dihydrosphingosine + a sugar
?
show the reaction diagram
Rhodococcus sp., Rhodococcus sp. M-777
-
-
-
-
?
D-erythro-sphingosine + a sugar
?
show the reaction diagram
Rhodococcus sp., Rhodococcus sp. M-777
-
-
-
-
?
daidzein-7-glucopyranoside + H2O
daidzein + beta-D-glucose
show the reaction diagram
Ovis aries aries
-
-
-
?
daidzin + H2O
?
show the reaction diagram
-
-
-
-
?
daidzin + H2O
3-(4-hydroxyphenyl)-7-hydroxy-chromen-4-one + D-glucose
show the reaction diagram
-
-
-
-
?
galactosyl-N-palmitoylceramide + H2O
beta-D-galactose + N-palmitoylceramide
show the reaction diagram
-
-
-
?
galactosyl-N-palmitoyldihydroceramide + H2O
beta-D-galactose + N-palmitoyldihydroceramide
show the reaction diagram
-
-
-
?
galactosylceramide + H2O
beta-D-galactose + ceramide
show the reaction diagram
-
-
-
?, ir
galactosylceramide + H2O
ceramide + D-galactose
show the reaction diagram
-
-
-
-
?
galactosylsphingosine + H2O
beta-D-galactose + sphingosine
show the reaction diagram
-
very low activity
-
?
galactosylsphingosine + H2O
sphingosine + D-galactose
show the reaction diagram
-
-
-
-
?
genistein-7-glucopyranoside + H2O
genistein + beta-D-glucose
show the reaction diagram
Ovis aries aries
-
-
-
?
genistein-7-glucoside + H2O
genistein + D-glucose
show the reaction diagram
-
-
-
-
?
glucosyl-N-lignoceroyldihydroceramide + H2O
beta-D-glucose + N-lignoceroyldihydroceramide
show the reaction diagram
-
-
-
?
glucosyl-N-palmitoylceramide + H2O
beta-D-glucose + N-palmitoylceramide
show the reaction diagram
-
-
-
?
glucosyl-N-palmitoyldihydroceramide + H2O
beta-D-glucose + N-palmitoyldihydroceramide
show the reaction diagram
-
-
-
?
glucosyl-N-stearoyldihydroceramide + H2O
beta-D-glucose + N-stearoyldihydroceramide
show the reaction diagram
-
-
-
?
glucosylceramide + H2O
beta-D-glucose + ceramide
show the reaction diagram
-
-
-
?
glucosylceramide + H2O
ceramide + D-glucose
show the reaction diagram
-
no activity with alpha-galactosylceramide, lactosylceramide, ganglioside M1a and sulfatides
-
-
?
glucosylsphingosine + H2O
beta-D-glucose + N-acylsphingosine
show the reaction diagram
-
very low activity
-
?
glucosylsphingosine + H2O
sphingosine + D-glucose
show the reaction diagram
-
-
-
-
?
glycosyl-N-acylsphingosine + H2O
N-acylsphingosine + a sugar
show the reaction diagram
-
-
-
-
?
glycosyl-N-acylsphingosine + H2O
N-acylsphingosine + a sugar
show the reaction diagram
-
-
-
-
?
glycosyl-N-acylsphingosine + H2O
N-acylsphingosine + a sugar
show the reaction diagram
-
-
-
-
?
lacto-N-tetraose + H2O
beta-D-glucose + ?
show the reaction diagram
-
-
-
?
lactose + H2O
?
show the reaction diagram
-
-
-
-
?
lactose + H2O
D-glucose + D-galactose
show the reaction diagram
-
-
-
-
?
lactose + H2O
D-glucose + D-galactose
show the reaction diagram
-
-
-
-
?
lactose + H2O
D-galactose + glucose
show the reaction diagram
-
reaction is not inhibited by 2-fluoro-dinitrophenylglucoside
-
-
?
lactosyl-N-lignoceroyldihydroceramide + H2O
beta-D-glucose + D-galactose + N-lignoceroyldihydroceramide
show the reaction diagram
-
-
-
?
lactosyl-N-palmitoyldihydroceramide + H2O
beta-D-glucose + D-galactose + N-palmitoyldihydroceramide
show the reaction diagram
-
-
-
?
lactosyl-N-stearoyldihydroceramide + H2O
beta-glucose + D-galactose + N-stearoyldihydroceramide
show the reaction diagram
-
-
-
?
N-palmitoyldihydroglucosylceramide + H2O
beta-D-glucose + ?
show the reaction diagram
-
-
-
?
N-palmitoyldihydroglucosylcerebroside + H2O
beta-D-glucose + N-palmitoyldihydrocerebroside
show the reaction diagram
-
1.5% of the activity towards lactose
-
?
p-nitrophenyl-beta-cellobioside + H2O
p-nitrophenyl-beta-D-glucoside + D-glucose
show the reaction diagram
-
-
-
-
?
p-nitrophenyl-beta-lactoside + H2O
p-nitrophenyl-beta-D-glucoside + D-galactose
show the reaction diagram
-
-
-
-
?
p-nitrophenyl-beta-maltoside + H2O
p-nitrophenol-beta-D-glucoside + D-glucose
show the reaction diagram
-
-
-
-
?
phenyl-beta-D-galactopyranoside + H2O
phenol + beta-D-galactopyranose
show the reaction diagram
-
low activity
-
?
phloretin-beta-D-galactoside + H2O
phloretin + beta-D-galactose
show the reaction diagram
-
very low activity
-
?
phlorizin + H2O
phloretin + beta-D-glucose
show the reaction diagram
-
-
-
?
phlorizin + H2O
phloretin + beta-D-glucose
show the reaction diagram
-
-
-
?
phlorizin + H2O
phloretin + beta-D-glucose
show the reaction diagram
-
-
-
-
?
phlorizin + H2O
phloretin + beta-D-glucose
show the reaction diagram
-
-
-
?
phlorizin + H2O
phloretin + beta-D-glucose
show the reaction diagram
-
-
-
?
phlorizin + H2O
phloretin + beta-D-glucose
show the reaction diagram
-
-
-
?
phlorizin + H2O
phloretin + beta-D-glucose
show the reaction diagram
-
-
-
?
phlorizin + H2O
phloretin + beta-D-glucose
show the reaction diagram
-
low activity
-
?
phlorizin + H2O
phloretin + beta-D-glucose
show the reaction diagram
-
low activity
-
?
phlorizin + H2O
phloretin + beta-D-glucose
show the reaction diagram
-
only substrate that is hydrolysed at the active site for phlorizin hydrolysis
-
?
phlorizin + H2O
phloretin + beta-D-glucose
show the reaction diagram
-
5% of the activity of lactose hydrolysis, hydrolysis located on a specific active site for phlorizin hydrolysis
-
?
phlorizin + H2O
phloretin + beta-D-glucose
show the reaction diagram
-
hydrolysis located on a specific active site for phlorizin hydrolysis
-
?
phlorizin + H2O
phloretin + beta-D-glucose
show the reaction diagram
Ovis aries aries
-
hydrolysis located on a specific active site for phlorizin hydrolysis
-
?
phlorizin + H2O
?
show the reaction diagram
-
-
-
-
?
phlorizin + H2O
?
show the reaction diagram
-
reaction is inhibited by 2-fluoro-dinitrophenylglucoside
-
-
?
pyridoxine-5'-beta-D-glucoside + H2O
pyridoxine + beta-D-glucose
show the reaction diagram
-
-
-
-
r
quercetin 3-O-beta-D-glucopyranoside + H2O
quercetin + beta-D-glucose
show the reaction diagram
Ovis aries aries
-
-
-
?
quercetin 4'-O-beta-D-glucopyranoside + H2O
quercetin + beta-D-glucose
show the reaction diagram
Ovis aries aries
-
flavone glucosides more efficiently hydrolysed than isoflavone glucosides, major contribution to the hydrolysis is from the lactase site
-
?
quercetin-3-glucoside + H2O
quercetin + D-glucose
show the reaction diagram
-
reaction is marginally inhibited by 2-fluoro-dinitrophenylglucoside
-
-
?
quercetin-4'-glucoside + H2O
quercetin + D-glucose
show the reaction diagram
-
reaction is marginally inhibited by 2-fluoro-dinitrophenylglucoside
-
-
?
lactosylceramide + H2O
beta-D-glucose + galactosylceramide
show the reaction diagram
-
low activity
-
?
additional information
?
-
-
absorption of quercetin-3-glucoside requires hydrolysis by the enzyme, absorption of quercetin-4-glucoside requires both an interaction with sodium-dependent glucose transporter and hydrolysis by the enzyme
-
-
-
additional information
?
-
-
enzyme is a major determinant of intestinal absorption of quercetin-3-glucoside
-
-
-
additional information
?
-
-
enzyme plays a major role in metabolism of glycosylated phytochemicals
-
-
-
additional information
?
-
-
no substrate: rutin, naringin
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
(2-hydroxymethylphenyl)-beta-D-glucopyranoside + H2O
2-hydroxymethylphenol + beta-D-glucose
show the reaction diagram
-
low activity, salicin
-
?
3'-fucosyllactose + H2O
beta-D-glucose + 3-O-(6-deoxy-alpha-L-galactopyranosyl)-beta-D-galactopyranose
show the reaction diagram
-
only oligosaccharides containing galactose as the glycone in beta-linkage hydrolysed
-
?
4-O-beta-D-galactopyranosyl-D-fructoside + H2O
beta-D-galactose + D-fructose
show the reaction diagram
-
very low activity
-
?
4-O-beta-D-galactopyranosyl-D-glucopyranoside + H2O
D-galactose + beta-D-glucose
show the reaction diagram
-
-
-
?
4-O-beta-D-galactopyranosyl-D-glucopyranoside + H2O
D-galactose + beta-D-glucose
show the reaction diagram
-
-
-
?
4-O-beta-D-galactopyranosyl-D-glucopyranoside + H2O
D-galactose + beta-D-glucose
show the reaction diagram
-
-
-
?
4-O-beta-D-galactopyranosyl-D-glucopyranoside + H2O
D-galactose + beta-D-glucose
show the reaction diagram
-
-
-
?
4-O-beta-D-galactopyranosyl-D-glucopyranoside + H2O
D-galactose + beta-D-glucose
show the reaction diagram
-
-
-
?
4-O-beta-D-galactopyranosyl-D-glucopyranoside + H2O
D-galactose + beta-D-glucose
show the reaction diagram
-
-
-
?
4-O-beta-D-galactopyranosyl-D-glucopyranoside + H2O
D-galactose + beta-D-glucose
show the reaction diagram
-
-
-
?
4-O-beta-D-galactopyranosyl-D-glucopyranoside + H2O
D-galactose + beta-D-glucose
show the reaction diagram
-
-
-
?
4-O-beta-D-galactopyranosyl-D-glucopyranoside + H2O
D-galactose + beta-D-glucose
show the reaction diagram
-
best substrate
-
?
4-O-beta-D-galactopyranosyl-D-glucopyranoside + H2O
D-galactose + beta-D-glucose
show the reaction diagram
-
best substrate
-
?
4-O-beta-D-galactopyranosyl-D-glucopyranoside + H2O
D-galactose + beta-D-glucose
show the reaction diagram
-
best substrate
-
?
4-O-beta-D-galactopyranosyl-D-glucopyranoside + H2O
D-galactose + beta-D-glucose
show the reaction diagram
-
best substrate
-
?
4-O-beta-D-galactopyranosyl-D-glucopyranoside + H2O
D-galactose + beta-D-glucose
show the reaction diagram
-
best substrate
-
?
4-O-beta-D-galactopyranosyl-D-glucopyranoside + H2O
D-galactose + beta-D-glucose
show the reaction diagram
-
hydrolysis located on a specific active site for lactose hydrolysis
-
?
4-O-beta-D-galactopyranosyl-D-glucopyranoside + H2O
D-galactose + beta-D-glucose
show the reaction diagram
Ovis aries aries
-
hydrolysis located on a specific active site for lactose hydrolysis
-
?
4-O-beta-D-galactopyranosyl-D-glucopyranoside + H2O
D-galactose + beta-D-glucose
show the reaction diagram
-
4fold higher activity for the N- and O-glycosylated than for the N-glycosylated molecule
-
?
4-O-beta-D-galactopyranosyl-D-glucopyranoside + H2O
D-galactose + beta-D-glucose
show the reaction diagram
-
acts on dietary glycosides before absorption
-
?
4-O-beta-D-galactopyranosyl-D-glucopyranoside + H2O
D-galactose + beta-D-glucose
show the reaction diagram
Ovis aries aries
-
acts on dietary glycosides before absorption
-
?
4-O-beta-D-galactopyranosyl-D-glucopyranoside + H2O
D-galactose + beta-D-glucose
show the reaction diagram
-
two enzymatic activities, one for lactose- and one for phlorizin-and lactose-hydrolysis
-
?
4-O-beta-D-galactopyranosyl-D-glucopyranoside + H2O
D-galactose + beta-D-glucose
show the reaction diagram
-
two enzymatic activities, one for lactose- and one for phlorizin-and lactose-hydrolysis
-
?
4-O-beta-D-galactopyranosyl-D-glucopyranoside + H2O
D-galactose + beta-D-glucose
show the reaction diagram
-
two enzymatic activities, one for lactose- and one for phlorizin-and lactose-hydrolysis
-
?
4-O-beta-D-galactopyranosyl-D-glucopyranoside + H2O
D-galactose + beta-D-glucose
show the reaction diagram
-
two enzymatic activities, one for lactose- and one for phlorizin-and lactose-hydrolysis
-
?
4-O-beta-D-galactopyranosyl-D-glucopyranoside + H2O
D-galactose + beta-D-glucose
show the reaction diagram
-
two enzymatic activities, one for lactose- and one for phlorizin-and lactose-hydrolysis
-
?
4-O-beta-D-galactopyranosyl-D-glucopyranoside + H2O
D-galactose + beta-D-glucose
show the reaction diagram
-
two enzymatic activities, one for lactose- and one for phlorizin-and lactose-hydrolysis
-
?
4-O-beta-D-galactopyranosyl-D-N-acetylglucosamine + H2O
beta-D-galactose + N-acetylglucosamine
show the reaction diagram
-
poor activity
-
?
4-O-beta-D-glucopyranosyl-D-glucose + H2O
beta-D-glucose + D-glucose
show the reaction diagram
-
cellobiose
-
?
4-O-beta-D-glucopyranosyl-D-glucose + H2O
beta-D-glucose + D-glucose
show the reaction diagram
-
cellobiose
-
?
4-O-beta-D-glucopyranosyl-D-glucose + H2O
beta-D-glucose + D-glucose
show the reaction diagram
-
cellobiose
-
?
4-O-beta-D-glucopyranosyl-D-glucose + H2O
beta-D-glucose + D-glucose
show the reaction diagram
-
cellobiose
-
?
4-O-beta-D-glucopyranosyl-D-glucose + H2O
beta-D-glucose + D-glucose
show the reaction diagram
-
cellobiose
-
?
4-O-beta-D-glucopyranosyl-D-glucose + H2O
beta-D-glucose + D-glucose
show the reaction diagram
-
cellobiose
-
?
4-O-beta-D-glucopyranosyl-D-glucose + H2O
beta-D-glucose + D-glucose
show the reaction diagram
-
cellobiose
-
?
6-O-beta-D-glucopyranosyl-D-glucose + H2O
beta-D-glucose + D-glucose
show the reaction diagram
-
low activity
-
?
6-O-beta-D-glucopyranosyl-D-glucose + H2O
beta-D-glucose + D-glucose
show the reaction diagram
-
low activity
-
?
6-O-beta-D-glucopyranosyl-D-glucose + H2O
beta-D-glucose + D-glucose
show the reaction diagram
-
gentiobiose
-
?
6-O-beta-D-glucopyranosyl-D-glucose + H2O
beta-D-glucose + D-glucose
show the reaction diagram
-
gentiobiose
-
?
6-O-beta-D-glucopyranosyl-D-glucose + H2O
beta-D-glucose + D-glucose
show the reaction diagram
-
gentiobiose
-
?
cellotetraose + H2O
? + beta-D-glucose
show the reaction diagram
-
considerable activity
-
?
cellotriose + H2O
? + beta-D-glucose
show the reaction diagram
-
considerable activity
-
?
cellulose + H2O
? + beta-D-glucose
show the reaction diagram
-
very low activity
-
?
galactosylceramide + H2O
beta-D-galactose + ceramide
show the reaction diagram
-
-
-
ir
galactosylsphingosine + H2O
beta-D-galactose + sphingosine
show the reaction diagram
-
very low activity
-
?
glucosylceramide + H2O
beta-D-glucose + ceramide
show the reaction diagram
-
-
-
?
glucosylsphingosine + H2O
beta-D-glucose + N-acylsphingosine
show the reaction diagram
-
very low activity
-
?
lacto-N-tetraose + H2O
beta-D-glucose + ?
show the reaction diagram
-
-
-
?
N-palmitoyldihydroglucosylceramide + H2O
beta-D-glucose + ?
show the reaction diagram
-
-
-
?
phlorizin + H2O
phloretin + beta-D-glucose
show the reaction diagram
-
-
-
?
phlorizin + H2O
phloretin + beta-D-glucose
show the reaction diagram
-
-
-
?
phlorizin + H2O
phloretin + beta-D-glucose
show the reaction diagram
-
-
-
?
phlorizin + H2O
phloretin + beta-D-glucose
show the reaction diagram
-
-
-
?
phlorizin + H2O
phloretin + beta-D-glucose
show the reaction diagram
-
-
-
?
phlorizin + H2O
phloretin + beta-D-glucose
show the reaction diagram
-
-
-
?
phlorizin + H2O
phloretin + beta-D-glucose
show the reaction diagram
-
low activity
-
?
phlorizin + H2O
phloretin + beta-D-glucose
show the reaction diagram
-
low activity
-
?
phlorizin + H2O
phloretin + beta-D-glucose
show the reaction diagram
-
only substrate that is hydrolysed at the active site for phlorizin hydrolysis
-
?
phlorizin + H2O
phloretin + beta-D-glucose
show the reaction diagram
-
5% of the activity of lactose hydrolysis, hydrolysis located on a specific active site for phlorizin hydrolysis
-
?
phlorizin + H2O
phloretin + beta-D-glucose
show the reaction diagram
-
hydrolysis located on a specific active site for phlorizin hydrolysis
-
?
phlorizin + H2O
phloretin + beta-D-glucose
show the reaction diagram
Ovis aries aries
-
hydrolysis located on a specific active site for phlorizin hydrolysis
-
?
phlorizin + H2O
?
show the reaction diagram
-
-
-
-
?
lactosylceramide + H2O
beta-D-glucose + galactosylceramide
show the reaction diagram
-
low activity
-
?
additional information
?
-
-
absorption of quercetin-3-glucoside requires hydrolysis by the enzyme, absorption of quercetin-4-glucoside requires both an interaction with sodium-dependent glucose transporter and hydrolysis by the enzyme
-
-
-
additional information
?
-
-
enzyme is a major determinant of intestinal absorption of quercetin-3-glucoside
-
-
-
additional information
?
-
-
enzyme plays a major role in metabolism of glycosylated phytochemicals
-
-
-
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
2',4'-dinitrophenyl-2-deoxy-2-fluoro-beta-D-galactopyranoside
-
inactivation of lactase hydrolase activity
2',4'-dinitrophenyl-2-deoxy-2-fluoro-beta-D-glucopyranoside
-
inactivation of phlorizin hydrolase activity
2',4'-dinitrophenyl-2-fluoro-2-deoxy-beta-D-glucopyranoside
Ovis aries aries
-
rapidly inhibits phlorizin hydrolase activity by covalently binding to the glutamic acid residue of the active site, lactase activity more slowly inhibited, pre-incubation with glucal protects the lactase active site from inactivation
2-fluoro-dinitrophenylglucoside
-
specifically inhibits phlorizin hydrolase, lactase activity is inhibited slowly
2-oxoglutaric acid
-
50% inhibition of the tartaric acid-activated phlorizin hydrolase at 20 mM
cellobiose
-
inhibition of galactosylceramide hydrolysis
cerebroside
-
from brain, competitive inhibition of lactase activity, Ki: 1.11 mM
conduritol-B-epoxide
-
90% inactivation of lactase hydrolysis, 55% inactivation of phlorizin hydrolysis, inactivation by covalent binding to Glu1747 and Glu 1271
D-Galactal
-
inactivation of lactase hydrolase activity
D-Galactono-1,4-lactone
-
competitive inhibition of lactase activity, Ki: 0.35 mM
D-Galactono-1,4-lactone
-
competitive inhibition of lactase activity, not phlorizin activity, Ki: 2 mM
D-glucal
-
inactivation of lactase hydrolase activity
D-glucono-1,5-lactone
-
competitive inhibition of lactase activity, Ki: 0.027 mM
D-glucono-1,5-lactone
-
competitive inhibition of phlorizin hydrolysis, Ki: 0.18 mM, competitive inhibition of lactase activity, Ki: 0.275 mM
deoxygalactonojirimycin
-
competitive inhibition, Ki: 0.076 mM
deoxynojirimycin
-
competitive inhibition, Ki: 0.142 mM
epigallocatechin-3-gallate
-
inhibits in vitro hydrolysis of lactose by lactase phlorizin hydrolase
galactosylceramide
-
competitive inhibition of glucosyldihydroceramide hydrolysis
glucosyldihydroceramide
-
competitive inhibition of galactosylceramide hydrolysis
lactose
-
competitive to pyridoxine-5'-beta-D-glucoside
N-(n-butyl)-deoxygalactojirimycin
-
-
n-butyldeoxygalactonojirimycin
-
-
NaCl
-
30% inhibition of the tartaric acid-stimulated phlorizin hydrolase at 20 mM
NSP4
-
secreted non-structural rotavirus protein
-
oxalacetic acid
-
50% inhibition of the tartaric acid-stimualted phlorizin hydrolase at 20 mM
oxalic acid
-
47% inhibition of the tartaric acid-activated phlorizin hydrolase at 20 mM
phlorizin
-
competitive inhibition of lactase activity; Ki: 0.44 mM
phlorizin
-
competitive inhibition of lactase activity
phlorizin
-
competitive inhibition of lactase activity, Ki: 0.37 mM
phlorizin
-
competitive inhibition of lactase activity, Ki: 0.18 mM
phlorizin
-
competitive inhibition of galactosylceramide hydrolysis, Ki: 0.35 mM
phlorizin
-
competitive inhibition of lactase activity, Ki: 0.047 mM
pyridoxine-5'-beta-D-glucoside
-
substrate inhibition above 3 mM
Pyruvic acid
-
strongly inhibitory for the tartaric acid-stimulated phlorizin hydrolase
SO42-
-
strong inhibition of the tartaric acid-activated phlorizin hydrolase at 20 mM
Sodium cholate
-
competitive inhibition of lactase activity, in the presence of bovine serum albumin
Tris(hydroxymethyl)aminomethane
-
competitive inhibition of lactase acitiviy, phlorizin activity not affected, Ki: 12 mM
Tris(hydroxymethyl)aminomethane
-
-
Tris(hydroxymethyl)aminomethane
-
competitive inhibition of lactase activity, Ki: 5.1 mM
Tris(hydroxymethyl)aminomethane
-
competitive inhibition of lactase activity, Ki: 4.5 mM
Maleic acid
-
55% inhibition for the tartaric acid-stimulated phlorizin hydrolase at 20 mM
additional information
-
not inhibitory: pyridoxine, 2,4-dinitrophenyl-2-fluoro-2-deoxy-beta-D-glucopyranoside
-
additional information
-
conduritol B epoxide only slightly inhibitory
-
additional information
-
LPH expression appears to fall off in the villus tip of germfree pigs. LPH activity to gene expression ratio is lower in pigs conventionalized with faecal bacteria or monoassociatied with Escherichia coli compared with germfree pigs. LPH activity to gene expression ratio in pigs monoassociatied with Lactobacillus fermentum is similar to germfree pigs whereas in pigs monoassociatied with Lactobacillus fermentum and contaminated with Klebsiella pneumoniae the ratio is similar to pigs monoassociatied with Escherichia coli
-
additional information
-
coexpression with Rab4S22N or Rab11S25N decreases the number of vesicular structures positive for LPH. Trafficking delay of LPH following Rab protein depletion in MDCK cells
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
cis-aconitic acid
-
more than 2fold stimulation only for phlorizin activity
Citric acid
-
highest stimulation at 50 mM and 30C only for phlorizin activity
isocitric acid
-
1.7fold stimulation only for phlorizin activity
L-ascorbic acid
-
1.6fold stimulation only for phlorizin activity
Pyruvic acid
-
0.5fold stimulation only for phlorizin activity
Sodium cholate
-
increases the solubility of the substrates
taurodeoxycholate
-
activation
L-Tartaric acid
-
most stimulating effect only for phlorizin activity
additional information
-
higher expression and gene transcription of LPH in jejunum of rats fed a high-carbohydrate/low-fat diet compared with those fed a low carbohydrate/high-fat diet. In vitro binding of the caudal homeobox protein Cdx-2, which is important for intestinal gene expression and differentiation, to the homeodomain protein-binding element CE-LPH1 in jejunal nuclear extracts is associated with the higher expression of LPH
-
additional information
-
presence of Rab8T22NCFP results in an increase in LPHYFP-containing vesicular structures
-
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
4.64
-
(6-[N-(7-nitrobenz-2-oxa-1,3 diazol-4-yl)amino]hexanoyl)-beta-D-glucosylceramide
-
-
13.1
-
2-nitrophenyl-beta-D-galactopyranoside
-
-
0.45
-
4-methylumbelliferyl-alpha-L-arabinoside
-
-
-
144.5
-
4-methylumbelliferyl-beta-D-galactopyranoside
-
-
0.24
-
4-Methylumbelliferyl-beta-D-galactoside
-
-
49.28
-
4-methylumbelliferyl-beta-D-glucopyranoside
-
-
0.53
-
4-methylumbelliferyl-beta-D-glucoside
-
-
1.4
-
4-methylumbelliferyl-beta-D-xyloside
-
-
0.36
-
4-nitrophenyl-beta-D-fucoside
-
-
22.3
-
4-nitrophenyl-beta-D-galactopyranoside
-
-
4.1
-
4-nitrophenyl-beta-D-glucopyranoside
-
-
67
-
4-O-beta-D-galactopyranosyl-D-fructoside
-
-
5.1
-
4-O-beta-D-galactopyranosyl-D-glucopyranoside
-
slightly higher catalytic efficiency than for the human enzyme
8.8
-
4-O-beta-D-galactopyranosyl-D-glucopyranoside
Ovis aries aries
-
-
14
-
4-O-beta-D-galactopyranosyl-D-glucopyranoside
-
both mannose-rich precursor form and mature form
14.8
-
4-O-beta-D-galactopyranosyl-D-glucopyranoside
-
from proximal intestine isolated lactase
19.1
-
4-O-beta-D-galactopyranosyl-D-glucopyranoside
-
from distal intestine isolated lactase
21
-
4-O-beta-D-galactopyranosyl-D-glucopyranoside
-
-
21.8
-
4-O-beta-D-galactopyranosyl-D-glucopyranoside
-
-
28
-
4-O-beta-D-galactopyranosyl-D-glucopyranoside
-
-
40
-
4-O-beta-D-galactopyranosyl-D-glucopyranoside
-
-
41.7
-
4-O-beta-D-galactopyranosyl-D-glucopyranoside
-
-
4.8
-
4-O-beta-D-galactopyranosyl-D-glucosamine
-
-
1.1
-
4-O-beta-D-glucopyranosyl-D-glucopyranoside
-
-
1.3
-
4-O-beta-D-glucopyranosyl-D-glucopyranoside
-
-
2.78
-
4-O-beta-D-glucopyranosyl-D-glucopyranoside
-
-
4.4
-
4-O-beta-D-glucopyranosyl-D-glucospyranoside
-
-
-
22
-
6-O-beta-D-glucopyranosyl-D-glucopyranoside
-
-
9.24
-
C18-galactosylceramide
-
-
13.67
-
C18-glucosylceramide
-
-
2.04
-
C6-4-nitrobenzo-2-oxa-1,3-diazole-galctosylceramide
-
-
0.3
-
D-erythro-dihydrosphingosine
-
-
0.04
-
D-erythro-sphingosine
-
-
0.149
-
daidzein-7-glucopyranoside
Ovis aries aries
-
-
0.149
-
daidzein-7-glucoside
-
pH 6, 37C
0.065
-
galactosylceramide
-
-
0.51
-
galactosylceramide
-
-
0.24
-
galactosylsphingosine
-
-
0.085
-
genistein-7-glucopyranoside
Ovis aries aries
-
-
0.085
-
genistein-7-glucoside
-
pH 6, 37C
0.01
-
glucosylceramide
-
-
0.62
-
glucosyldihydroceramide
-
-
0.37
-
glucosylsphingosine
-
-
8.8
-
lactose
-
pH 6, 37C
16
-
lactose
-
pH 6.0, 37C
28.9
-
lactose
-
in infected cells, in brush border-membrane enriched fraction
30
-
lactose
-
in non infected cells, in brush border-membrane enriched fraction
0.27
-
lactosylceramide
-
-
0.73
-
N-palmitoyldihydrocerebroside
-
from proximal intestine isolated phlorizin hydrolase activity
1.12
-
N-palmitoyldihydrocerebroside
-
from distal intestine isolated phlorizin hydrolase activity
0.542
-
p-nitrophenyl-beta-cellobioside
-
at 37C, pH 5.0
18.13
-
p-nitrophenyl-beta-cellobioside
-
at 37C, pH 7.4
1.67
-
p-nitrophenyl-beta-lactoside
-
at 37C, pH 5.0
21.62
-
p-nitrophenyl-beta-lactoside
-
at 37C, pH 7.4
2.82
-
p-nitrophenyl-beta-maltoside
-
at 37C, pH 5.0
5.12
-
p-nitrophenyl-beta-maltoside
-
at 37C, pH 7.4
0.4
-
phloretin-beta-D-galactoside
-
-
0.002
-
phlorizin
-
pH 6, 37C
0.002
-
phlorizin
Ovis aries aries
-
-
0.1
-
phlorizin
-
-
0.18
-
phlorizin
-
-
0.4
-
phlorizin
-
-
0.4
-
phlorizin
-
-
0.44
-
phlorizin
-
-
1
-
pyridoxine-5'-beta-D-glucoside
-
pH 6.0, 37C
0.046
-
quercetin-3-glucopyranoside
Ovis aries aries
-
-
0.046
-
quercetin-3-glucoside
-
pH 6, 37C
0.044
-
quercetin-4'-glucoside
-
pH 6, 37C
0.044
-
quercetin-4-glucopyranoside
Ovis aries aries
-
-
TURNOVER NUMBER [1/s]
TURNOVER NUMBER MAXIMUM[1/s]
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
33.8
-
4-O-beta-D-galactopyranosyl-D-glucopyranoside
Ovis aries aries
-
-
2.1
-
daidzein-7-beta-D-glucopyranoside
Ovis aries aries
-
-
2.1
-
daidzein-7-glucoside
-
pH 6, 37C
6.5
-
genistein-7-beta-D-glucopyranoside
Ovis aries aries
-
-
6.5
-
genistein-7-glucoside
-
pH 6, 37C
33.8
-
lactose
-
pH 6, 37C
0.5
-
phlorizin
-
pH 6, 37C
0.5
-
phlorizin
Ovis aries aries
-
-
1
-
pyridoxine-5'-beta-D-glucoside
-
pH 6.0, 37C
6.3
-
quercetin-3-beta-D-glucopyranoside
Ovis aries aries
-
-
6.3
-
quercetin-3-glucoside
-
pH 6, 37C
7.5
-
quercetin-4'-glucoside
-
pH 6, 37C
7.5
-
quercetin-4-beta-D-glucopyranoside
Ovis aries aries
-
-
Ki VALUE [mM]
Ki VALUE [mM] Maximum
INHIBITOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
1.11
-
cerebroside
-
from brain, competitive inhibition of lactase activity
64
-
D-glucose
-
pH 6.0, 37C
0.076
-
deoxygalactonojirimycin
-
competitive inhibition
0.142
-
deoxynojirimycin
-
competitive inhibition
56
-
lactose
-
pH 6.0, 37C
IC50 VALUE [mM]
IC50 VALUE [mM] Maximum
INHIBITOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.074
-
epigallocatechin-3-gallate
-
in 1.5 mM sodium phosphate buffer (pH 6.8), at 37C
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
0.00016
-
-
activity in CHOP cell lysates transformed with cDNA encoding KLPH, with conduritol B epoxide, C6-4-nitrobenzo-2-oxa-1,3-diazole-galactosylceramide as substrate
0.00025
-
-
activity in CHOP cell lysates transformed with cDNA encoding KLPH, without conduritol B epoxide, C6-4-nitrobenzo-2-oxa-1,3-diazole-galactosylceramide as substrate
0.0005
-
-
activity in CHOP cell lysates transformed with cDNA encoding KLPH, with conduritol B epoxide, C6-4-nitrobenzo-2-oxa-1,3-diazole-glucosylceramide as substrate
0.0005
-
-
in membrane fraction with conduritol B epoxide, C6-4-nitrobenzo-2-oxa-1,3-diazole-glucosylceramide as substrate
0.00133
-
-
in cytosolic fraction with conduritol B epoxide, C6-4-nitrobenzo-2-oxa-1,3-diazole-glucosylceramide as substrate
0.00164
-
-
in non infected cells, cell homogenates
0.00167
-
-
in cells infected with rhesus monkey rotavirus, cell homogenates
0.001875
-
-
activity in CHOP cell lysates transformed with cDNA encoding KlrP, with conduritol B epoxide, C6-4-nitrobenzo-2-oxa-1,3-diazole-glucosylceramide as substrate
0.00219
-
-
in cells infected with rhesus monkey rotavirus, in brush border-membrane enriched fraction
0.0025
-
-
activity in CHOP cell lysates transformed with cDNA encoding KLPH, without conduritol B epoxide, C6-4-nitrobenzo-2-oxa-1,3-diazole-glucosylceramide as substrate
0.0028
-
-
in cells infected with rhesus monkey rotavirus and NSP4 neutralizing antibodies, in brush border-membrane enriched fraction
0.00334
-
-
in non infected cells, in brush border-membrane enriched fraction
0.00359
-
-
in cells infected with inactivated rhesus monkey rotavirus, in brush border-membrane enriched fraction
0.00375
-
-
activity in CHOP cell lysates transformed with cDNA encoding KlrP, without conduritol B epoxide, C6-4-nitrobenzo-2-oxa-1,3-diazole-glucosylceramide as substrate
0.0052
-
-
activity in CHOP cell lysates transformed with cDNA encoding KlrP, with conduritol B epoxide, C6-4-nitrobenzo-2-oxa-1,3-diazole-galactosylceramide as substrate
0.0066
-
-
activity in CHOP cell lysates transformed with cDNA encoding KlrP, without conduritol B epoxide, C6-4-nitrobenzo-2-oxa-1,3-diazole-galactosylceramide as substrate
0.45
-
-
phlorizin activity
1.78
-
-
galactosylceramide hydrolysis
2.67
-
-
glucosylceramide hydrolysis
5
6
-
lactase activity
15.33
-
-
lactose hydrolysis
16.1
-
-
lactase activity
20
-
-
-
22
-
-
-
32
-
-
-
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
5.2
5.6
-
phlorizin activity in acetate buffer
5.2
-
-
hydrolysis of galactosyl- or glucosylceramide in the presence of taurodeoxycholate
5.5
5.7
-
hydrolysis of disaccharides or artificial substrates
5.5
-
-
-
5.6
6.2
-
-
5.6
-
-
lactase activity, phlorizin activity in the acidic pH range
5.6
-
-
phlorizin activity at pH 3.3-3.5 in citrate buffer
5.8
6
-
-
6
7
-
C6-4-nitrobenzo-2-oxa-1,3-diazole-glucosylceramide as substrate
6
-
-
both isoforms
6
-
-
-
7.5
-
-
assay at
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
37
-
-
assay at
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
-
small intestine
Manually annotated by BRENDA team
-
LPH activity to gene expression ratio is lower in pigs conventionalized with faecal bacteria or monoassociatied with Escherichia coli compared with germfree pigs. LPH activity to gene expression ratio in pigs monoassociatied with Lactobacillus fermentum is similar to germfree pigs whereas in pigs monoassociatied with Lactobacillus fermentum and contaminated with Klebsiella pneumoniae the ratio is similar to pigs monoassociatied with Escherichia coli
Manually annotated by BRENDA team
Ovis aries aries
-
small intestine
Manually annotated by BRENDA team
-
highest activity in proximal jejenum, lowest in proximal and distal ends of the small intestine
Manually annotated by BRENDA team
-
small intestine
Manually annotated by BRENDA team
-
proximal jejenum; small intestine
Manually annotated by BRENDA team
-
small intestine
Manually annotated by BRENDA team
-
small intestine
Manually annotated by BRENDA team
-
intestinal brush border enzyme
Manually annotated by BRENDA team
-
everted sac preparation
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
Ovis aries aries
-
luminal site
Manually annotated by BRENDA team
-
anchored via the C-terminal hydrophobic stretch in C(in)-N(out) orientation
Manually annotated by BRENDA team
-
transfected Cos-1 cells
Manually annotated by BRENDA team
additional information
-
sequential passage of LPH through Rab4-, Rab8- and Rab11-containing compartments. Trafficking of LPH from the TGN to the cell surface can be modulated by dominant-negative Rab8
-
Manually annotated by BRENDA team
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
38000
-
-
sequence analysis
57000
-
-
SDS-PAGE
130000
-
-
mature form, proteolytically cleaved from precursor form, SDS-PAGE
135000
-
-
SDS-PAGE
145000
-
-
SDS-PAGE, mature form
160000
-
-
cleaved mature form, SDS-PAGE
160000
-
-
cleaved mature form, SDS-PAGE
160000
-
-
cleaved mature form, SDS-PAGE
160000
-
-
SDS-PAGE, cleaved mature form
215000
-
-
mannose-rich form, SDS-PAGE
215000
-
-
mannose-rich precursor form, SDS-PAGE
220000
-
-
single-chain precursor, subsequently proteolytically processed to its active form, SDS-PAGE
220000
-
-
single-chain precursor, subsequently proteolytically processed to its active form, SDS-PAGE
220000
-
-
SDS-PAGE, high mannose form
230000
-
-
mannose-rich form, SDS-PAGE
245000
-
-
high-mannose precursor form, SDS-PAGE
245000
-
-
high-mannose precursor form, SDS-PAGE
280000
-
-
gel filtration, hydrophilic form
290000
300000
-
gel filtration
320000
-
-
gel filtration, amphiphilic form
320000
-
-
gel filtration, amphiphilic form
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
?
-
x * 135000-140000, SDS-PAGE, mature protein
dimer
-
2 * 160000, SDS-PAGE
dimer
-
-
dimer
-
2 * 160000, SDS-PAGE
monomer
-
1 * 135000, SDS-PAGE
monomer
-
1 * 130000, SDS-PAGE
monomer
-
1 * 160000, SDS-PAGE, mature brush-border form after proteolytic cleavage of the precursor form
monomer
-
1 * 160000, SDS-PAGE, mature brush-border form after proteolytic cleavage of the precursor form
additional information
-
region LAC236 between R1647 and T1882 is implicated in the dimerization processof pro-enzyme
additional information
-
phlorizin hydrolysis occurs at region III of enzyme, lactase hydrolysis occurs at region IV
additional information
-
LPH encompasses four homologous domains, which presumably have evolved from two subsequent duplications of one ancestral gene, domain organization, overview. The profragment, LPHalpha, comprises homologous domains I and II and functions as an intramolecular chaperone in the context of the brush-border LPHbeta region of LPH. Inter-relationship between homologous domains III and IV of LPHbeta implication in the overall structure, function, and trafficking of LPH, overview. Isolated homologous domain III is transport-competent per se and sorted to the apical membrane in polarized cells. Nevertheless, it neither dimerizes nor acquires complete phlorizin hydrolase activity
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
glycoprotein
-
various modified glycoforms do not affect the transport from the Golgi to the cell surface
glycoprotein
-
mature LPH O- and N-glycosylated, no sialic acids
glycoprotein
-
processing of enzyme to a complex glycosylated protein increases in presence of pro-region
proteolytic modification
-
pro-enzyme is cleaved in trans-Golgi network between Arg734 and Leu735, pro-region of enzyme is an intramolecular chaperone, analysis of processing and folding
proteolytic modification
-
precursor form of 200000-220000 Da
glycoprotein
-
17% carbohydrate content, consisting of galactose, mannose, fucose, N-acetylglucosamine
glycoprotein
-
endo H treatment
proteolytic modification
-
oral doses of insulin-like growth factor I increase the processing efficiency of pro-enzyme but do not affect enzyme activity
Crystallization/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
of the recombinant protein with the hanging drops by vapor diffusion method
-
by vapor diffusion in the presence of detergents like Triton X-100, octyl beta-D-glucopyranoside, decyl beta-D-maltopyranoside, and dodecyl beta-D-maltopyranoside, form, in complex with the ganglioside GM3
-
pH STABILITY
pH STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
3.2
-
-
little activity below
4.2
9
-
little activity outside this range
TEMPERATURE STABILITY
TEMPERATURE STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
4
-
-
no detectable loss of activity for several weeks
45
-
-
more than 90% of activity lost within 20 min
45
-
-
stable for 60 min
46
-
-
34-70% remaining activity after 30 min, depending on substrates
49
-
-
lactase activity faster destroyed than phlorizin activity
55
-
-
complete inactivation
GENERAL STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
sensitive to repeated freezing and thawing
-
at neutral pH and 37C, lactase acitivity rapidly lost, partially prevented by p-chloromercuribenzoate, more stable if attached to its border-membrane
-
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
-20C, several months
-
-20C, several months
-
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
by magnesium precipitation method
-
Helix pomatia lectin chromatography
-
of the recombinant protein by a hi-trap chelating HP column and superdex 200 column chromatography
-
to homogeneity, immunoadsorbent chromatography
-
highly purified
-
to homogeneity, chromatography techniques
-
to homogeneity, chromatography steps
-
antibody affinity chromatography
-
to homogeneity, chromatography techniques
-
to homogeneity
Ovis aries aries
-
to homogeneity, immunoaffinity chromatography
-
two isoenzymes from proximal and distal intestine, to homogeneity, chromatography techniques
-
of the recombinant protein by by Ni(II) affinity chromatography
-
to homogeneity, immunoadsorbent chromatography, amphiphilic form
-
Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
COS-1 cells triple transfected with LPHCFP, SIYFP and Rab4, Rab8 or Rab11 fused to DsRed. MDCK cells stably expressing LPHmyc
-
expression in Chinese hamster ovary-derived cells expressing polyoma LT antigen, in HEK293 cells cotransfected with in KlrP siRNA, overexpression in Escherichia coli
-
expression in Cos-1 cells
-
expression of mutants in Cos-7 cells
-
genotyping
-
mutant G1363S introduced into the wild-type LPH cDNA cloned in the vector pSG5. Mutant transiently expressed in COS-1 cells
-
transient expression of wild-type and domain deletion mutant enzymes and isolated enzyme domains in Cos-1 cells, expression in MDCK cells
-
expression in Chinese hamster ovary-derived cells expressing polyoma LT antigen
-
expressed in CACO-2 cells; LPH 5' upstream subcloned from rat liver genomic DNA
-
expression of the gene lacking the 30-residue N-terminal secretion signal sequence in Escherichia coli, selenomethionine-derivatized enzyme
-
ENGINEERING
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
D1711N
-
introduction of a glycosylation site, reduced transport rate to the plasma membrane; introduction of potential N-glycosylation site, no enzymic activity, probably due to altered protein folding pattern and reduced dimerization efficiency
E165D
-
reduced activity
E165Q
-
almost no activity
E373D
-
reduced activity
E373Q
-
almost no activity
G1363S
-
the mutant protein is malfolded and enzymatically inactive and can not exit the endoplasmic reticulum. The mutation creates an additional N-glycosylation site that is characteristic of a temperature-sensitive protein. The potential glycosylation site generated by the mutation is not the cause of defective trafficking of LPH-G1363S or its reduced enzymatic activity. Intracellular transport and enzymatic activity, but not correct folding are partially restored by expression at 20C. The mutant is responsible for an increased turnover rate
G1363S/N1361A
-
eliminates the N-glycosylation site, does not restore the features of wild-type LPH
P1743S
-
introduction of a glycosylation site, reduced transport rate to the plasma membrane; introduction of potential N-glycosylation site, no enzymic activity, probably due to altered protein folding pattern and reduced dimerization efficiency
E233A
-
site directed mutagenesis
E351S
-
site directed mutagenesis
E233A
Rhodococcus sp. M-777
-
site directed mutagenesis
-
E351S
Rhodococcus sp. M-777
-
site directed mutagenesis
-
I1697N
-
introduction of a glycosylation site, reduced transport rate to the plasma membrane; introduction of potential N-glycosylation site, no enzymic activity, probably due to altered protein folding pattern and reduced dimerization efficiency
additional information
-
loop-out mutagenesis and construction of deletion or individual domain forms of LPH. Removal of domain IV, which contains lactase, results in a diminished phlorizin hydrolase activity, lack of dimerization in the endoplasmic reticulum, but accelerated transport kinetics from the endoplasmic reticulum to the Golgi apparatus. By contrast, deletion of domain III, which harbors phlorizin hydrolase, generates a malfolded protein that is blocked in the endoplasmic reticulum. Transport kinetics and enzyme activity of LPH and deletion mutants differ
additional information
-
the naturally occurring polymorphism T13910C, i.e. rs4988235, causes lactose intolerance in the C-variant, associated with lower dietary calcium intake and serum calcium levels but not with BMD or fractures. Genotyping and phenotype, overview. The polymorphism is not associated with the genetic variations in the VDR gene, and no interactions between the two genotypes
APPLICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
medicine
-
rotavirus infection impairs LPH activity in intestinal cells, the decrease in enzyme activity is not Ca2+- and cAMP-dependent, the LPH biosynthesis, stability, and expression is not modified, the impairment of lactase enzymatic activity during rotavirus infection results from an inhibitory action of the secreted non-structural rotavirus protein NSP4
medicine
-
LPH is a marker for the terminal differentiation phases of the intestinal development in mice, inactivation of gata 4 has no effect on the mRNA expression of LPH, inactivation of hnf1 results in 50% reduced mRNA levels, specific intestinal genes have differential requirements for gata 4 and hnf1
medicine
-
absorption of quercetin-3-glucoside requires hydrolysis by the enzyme, absorption of quercetin-4-glucoside requires both an interaction with sodium-dependent glucose transporter and hydrolysis by the enzyme
medicine
-
perfusion of jejunum and ileum of rats with quercetin-3-glucoseide, enzyme is involved in in vivo intestinal uptake of quercetin-sugars
medicine
-
enzyme plays a major role in metabolism of glycosylated phytochemicals
medicine
-
mutant form G1363S of LPH is involved in the pathogenesis of congenital lactase deficiency
additional information
-
regulation of LPH exocytosis by multiple Rab GTPases