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Literature summary for 3.2.1.62 extracted from

  • Hamaswamy, S.; Radhakrishnan, A.N.
    Lactase-phlorizin hydrolase complex from monkey small intestine: Stimulation of phlorizin hydrolase activity by organic acids (1973), Biochem. Biophys. Res. Commun., 54, 197-204.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
cis-aconitic acid more than 2fold stimulation only for phlorizin activity Macaca radiata
Citric acid highest stimulation at 50 mM and 30°C only for phlorizin activity Macaca radiata
isocitric acid 1.7fold stimulation only for phlorizin activity Macaca radiata
L-ascorbic acid 1.6fold stimulation only for phlorizin activity Macaca radiata
L-Tartaric acid most stimulating effect only for phlorizin activity Macaca radiata
Pyruvic acid 0.5fold stimulation only for phlorizin activity Macaca radiata

Inhibitors

Inhibitors Comment Organism Structure
2-oxoglutaric acid 50% inhibition of the tartaric acid-activated phlorizin hydrolase at 20 mM Macaca radiata
Maleic acid 55% inhibition for the tartaric acid-stimulated phlorizin hydrolase at 20 mM Macaca radiata
NaCl 30% inhibition of the tartaric acid-stimulated phlorizin hydrolase at 20 mM Macaca radiata
oxalacetic acid 50% inhibition of the tartaric acid-stimualted phlorizin hydrolase at 20 mM Macaca radiata
oxalic acid 47% inhibition of the tartaric acid-activated phlorizin hydrolase at 20 mM Macaca radiata
Pyruvic acid strongly inhibitory for the tartaric acid-stimulated phlorizin hydrolase Macaca radiata
SO42- strong inhibition of the tartaric acid-activated phlorizin hydrolase at 20 mM Macaca radiata

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-O-beta-D-galactopyranosyl-D-glucopyranoside + H2O Macaca radiata
-
D-galactose + beta-D-glucose
-
?
phlorizin + H2O Macaca radiata
-
phloretin + beta-D-glucose
-
?

Organism

Organism UniProt Comment Textmining
Macaca radiata
-
monkey
-

Purification (Commentary)

Purification (Comment) Organism
highly purified Macaca radiata

Source Tissue

Source Tissue Comment Organism Textmining
intestine small intestine Macaca radiata
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5 6 lactase activity Macaca radiata

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-O-beta-D-galactopyranosyl-D-glucopyranoside + H2O
-
Macaca radiata D-galactose + beta-D-glucose
-
?
phlorizin + H2O
-
Macaca radiata phloretin + beta-D-glucose
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.2 5.6 phlorizin activity in acetate buffer Macaca radiata
5.6
-
phlorizin activity at pH 3.3-3.5 in citrate buffer Macaca radiata