3.2.1.62: glycosylceramidase

This is an abbreviated version!
For detailed information about glycosylceramidase, go to the full flat file.

Reaction

Synonyms

cerebrosidase, EGC, EGCII, EGCrP1, Egh1, endo-glycoceramidase II, endoglycoceramidase II, endoglycoceramidase-related protein 1, ergosteryl-beta-glucosidase, Gcase, glucocerebrosidase, glycosyl ceramide glycosylhydrolase, glycosyl-N-acylsphingosine glycohydrolase, glycosylceramidase, KLotho lactase phlorizin hydrolase, Klotho-related protein, lactase phlorizin hydrolase, lactase-phlorizin hydrolase, LCT, LPH, LPH1, phloretin-glucosidase, phloridzin glucosidase, phlorizin beta-glucosidase, phlorizin hydrolase, Yir007w

ECTree

     3 Hydrolases

         3.2 Glycosylases

             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds

                3.2.1.62 glycosylceramidase

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Results	in table
11	Activating Compound
15	AA Sequence
7	Application
1	CAS Registry Number
16	Cloned(Commentary)
3	Crystallization (Commentary)
94	Disease/ Diagnostics
9	General Information
2	General Stability
1	IC50 Value
62	Inhibitors
4	kcat/KM [mM/s]
5	Ki Value [mM]
73	KM Value [mM]
27	Localization
1	Metals/Ions
27	Molecular Weight [Da]
76	Natural Substrates/ Products (Substrates)
2	Organic Solvent Stability
125	Organism
6	Pathway
15	pH Optimum
2	pH Range
2	pH Stability
13	Posttranslational Modification
23	Protein Variants
22	Purification (Commentary)
4	Reaction
9	Reaction Type
56	Reference
46	Source Tissue
26	Specific Activity [micromol/min/mg]
2	Storage Stability
198	Substrates and Products (Substrate)
16	Subunits
77	Synonyms
1	Systematic Name
3	Temperature Optimum [°C]
1	Temperature Range [°C]
8	Temperature Stability [°C]
17	Turnover Number [1/s]

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TURNOVER NUMBER [1/s]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

2.9 - 5.4

2-chloro-4-nitrophenyl beta-cellobioside

3 entries

33.8

4-O-beta-D-galactopyranosyl-D-glucopyranoside

Ovis aries aries

-

-

208743

0.0383

C6-7-nitro-2,1,3-benzoxadiazole-glucosylceramide

Cryptococcus neoformans

H1AE12

at pH 7.3 and 30°C

732073

2.1

daidzein-7-beta-D-glucopyranoside

Ovis aries aries

-

-

208743

2.1

daidzein-7-glucoside

Ovis aries

-

pH 6, 37°C

208743

6.5

genistein-7-beta-D-glucopyranoside

Ovis aries aries

-

-

208743

6.5

genistein-7-glucoside

Ovis aries

-

pH 6, 37°C

208743

33.8

lactose

Ovis aries

-

pH 6, 37°C

208743

0.5

phlorizin

2 entries

1

pyridoxine-5'-beta-D-glucoside

Rattus norvegicus

-

pH 6.0, 37°C

656061

6.3

quercetin 3-O-beta-D-glucopyranoside

Ovis aries aries

-

-

208743

6.3

quercetin-3-glucoside

Ovis aries

-

pH 6, 37°C

208743

7.5

quercetin-4'-glucoside

Ovis aries

-

pH 6, 37°C

208743

7.5

quercetin-4-beta-D-glucopyranoside

Ovis aries aries

-

-

208743

2.9

2-chloro-4-nitrophenyl beta-cellobioside

Rhodococcus sp. M-777

O33853

mutant enzyme D311Y, hydrolysis reaction, at pH 4.0 and 37°C

749451

3.7

2-chloro-4-nitrophenyl beta-cellobioside

Rhodococcus sp. M-777

O33853

wild type enzyme, hydrolysis reaction, at pH 4.0 and 37°C

749451

5.4

2-chloro-4-nitrophenyl beta-cellobioside

Rhodococcus sp. M-777

O33853

mutant enzyme D314Y, hydrolysis reaction, at pH 4.0 and 37°C

749451

0.5

phlorizin

Ovis aries aries

-

-

208743

0.5

phlorizin

Ovis aries

-

pH 6, 37°C

208743