Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

3.2.1.62: glycosylceramidase

This is an abbreviated version!
For detailed information about glycosylceramidase, go to the full flat file.

Word Map on EC 3.2.1.62

Reaction

a beta-D-galactosyl-N-acylsphingosine
+
H2O
=
a ceramide
+
beta-D-galactose

Synonyms

cerebrosidase, EGC, EGCII, EGCrP1, Egh1, endo-glycoceramidase II, endoglycoceramidase II, endoglycoceramidase-related protein 1, ergosteryl-beta-glucosidase, Gcase, glucocerebrosidase, glycosyl ceramide glycosylhydrolase, glycosyl-N-acylsphingosine glycohydrolase, glycosylceramidase, KLotho lactase phlorizin hydrolase, Klotho-related protein, lactase phlorizin hydrolase, lactase-phlorizin hydrolase, LCT, LPH, LPH1, phloretin-glucosidase, phloridzin glucosidase, phlorizin beta-glucosidase, phlorizin hydrolase, Yir007w

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.62 glycosylceramidase

Purification

Purification on EC 3.2.1.62 - glycosylceramidase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
antibody affinity chromatography
-
by magnesium precipitation method
-
GSTrap column chromatography
Helix pomatia lectin chromatography
-
highly purified
-
nickel affinity column chromatography
nickel-conjugated Sepharose 6 column chromatography and Superdex 200 gel filtration
-
of the recombinant protein by a hi-trap chelating HP column and superdex 200 column chromatography
-
of the recombinant protein by by Ni(II) affinity chromatography
-
TALON metal affinity resin column chromatography
to homogeneity
Ovis aries aries
-
to homogeneity, chromatography steps
-
to homogeneity, chromatography techniques
to homogeneity, immunoadsorbent chromatography
-
to homogeneity, immunoadsorbent chromatography, amphiphilic form
-
to homogeneity, immunoaffinity chromatography
-
two isoenzymes from proximal and distal intestine, to homogeneity, chromatography techniques
-