3.2.1.135: neopullulanase

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For detailed information about neopullulanase, go to the full flat file.

Word Map on EC 3.2.1.135

3.2.1.135

starch

stearothermophilus

amylolytic

alpha-amylases

cyclomaltodextrinase

maltogenic

amylopectin

transglycosylation

amylose

alpha-1,4

amylopullulanase

debranching

oligo-1,6-glucosidase

glucanotransferase

thermoactinomyces

alpha-1,6-glucosidic

isopanose

cdase

saccharifying

pullulanases

cyclodextrinase

maltooligosaccharides

isoamylase

isopullulanase

gamma-cyclodextrins

biotechnology

industry

synthesis

Reaction

Synonyms

Amo105, amylopullalanase, amylopullulanase, ApuA, ApuADELTA, bsNpl, cyclomaltodextrinase, Env Npu193A, More, neopullulanase-alpha-amylase, neopullulanase-like enzyme, Pul, pullulan 4-D-glucanohydrolase (6-alpha-D-glucosylmaltose), pullulan hydrolase type I, pullulanase, pullulanase II, pullulanase, neo-, Rbamy5, TetApuM955, TetApuR855, type II pullulanase, type III pullulan hydrolase

ECTree

3 Hydrolases

3.2 Glycosylases

3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds

3.2.1.135 neopullulanase

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Results	in table
1314	AA Sequence
5	Application
1	CAS Registry Number
14	Cloned(Commentary)
2	Crystallization (Commentary)
3	General Information
1	General Stability
19	Inhibitors
1	kcat/KM [mM/s]
12	KM Value [mM]
14	Localization
15	Metals/Ions
23	Molecular Weight [Da]
41	Natural Substrates/ Products (Substrates)
86	Organism
43	PDB ID
16	pH Optimum
6	pH Range
5	pH Stability
41	Protein Variants
11	Purification (Commentary)
5	Reaction
8	Reaction Type
65	Reference
2	Source Tissue
18	Specific Activity [micromol/min/mg]
1	Storage Stability
169	Substrates and Products (Substrate)
23	Subunits
60	Synonyms
1	Systematic Name
17	Temperature Optimum [°C]
5	Temperature Range [°C]
15	Temperature Stability [°C]
7	Turnover Number [1/s]

Purification

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Purification on EC 3.2.1.135 - neopullulanase

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5 entries

ammonium sulfate precipitation, DEAE-Sepharose column chromatography, and Sephadex G-100 gel filtration

Geobacillus stearothermophilus

703502

BD TALON spin column for 6x His-tag protein affinity purification, simultaneous SDS-PAGE zymogram with 1% soluble starch

Thermoanaerobacter ethanolicus

697834

gene purification by agarose gel electrophoresis, enzyme purification by size exclusion chromatography, elution via Superdex 200 10/300 GL column with 50 mM MOPS, pH7, 50 mM NaCl

uncultured microorganism

A7LI67

696810

Lactococcus lactis cytoplasmic fraction containing the alpha-amylase and pullulanase domain proteins, respectively, is purified by using Ni-nitrilotriacetic acid matrices, elution fractions analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis

Bifidobacterium breve

695738

recombinant enzyme

Geobacillus stearothermophilus

Q9AIV2

646820

supernatant ultrafiltrated, DEAE Sepharose CLl-4B, Superose 6

Lactiplantibacillus plantarum

699051