3.2.1.135: neopullulanase

This is an abbreviated version!
For detailed information about neopullulanase, go to the full flat file.

Reaction

Synonyms

Amo105, amylopullalanase, amylopullulanase, ApuA, ApuADELTA, bsNpl, cyclomaltodextrinase, Env Npu193A, More, neopullulanase-alpha-amylase, neopullulanase-like enzyme, Pul, pullulan 4-D-glucanohydrolase (6-alpha-D-glucosylmaltose), pullulan hydrolase type I, pullulanase, pullulanase II, pullulanase, neo-, Rbamy5, TetApuM955, TetApuR855, type II pullulanase, type III pullulan hydrolase

ECTree

     3 Hydrolases

         3.2 Glycosylases

             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds

                3.2.1.135 neopullulanase

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Results	in table
1314	AA Sequence
5	Application
1	CAS Registry Number
14	Cloned(Commentary)
2	Crystallization (Commentary)
3	General Information
1	General Stability
19	Inhibitors
1	kcat/KM [mM/s]
12	KM Value [mM]
14	Localization
15	Metals/Ions
23	Molecular Weight [Da]
41	Natural Substrates/ Products (Substrates)
86	Organism
43	PDB ID
16	pH Optimum
6	pH Range
5	pH Stability
41	Protein Variants
11	Purification (Commentary)
5	Reaction
8	Reaction Type
65	Reference
2	Source Tissue
18	Specific Activity [micromol/min/mg]
1	Storage Stability
169	Substrates and Products (Substrate)
23	Subunits
60	Synonyms
1	Systematic Name
17	Temperature Optimum [°C]
5	Temperature Range [°C]
15	Temperature Stability [°C]
7	Turnover Number [1/s]

General Information

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General Information on EC 3.2.1.135 - neopullulanase

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GENERAL INFORMATION

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

evolution

Geobacillus stearothermophilus

P38940

analysis and verification of neopullulanases belonging to the alpha-amylase family GH13, subfamily GH13_20, but also subfamily GH13_36, an intermediary group, which is defined using the sequence of the fifth conserved sequence region (CSR) as a selection marker. Evolutionary relationships and tree, and conserved sequence regions of GH13 subfamilies, detailed overview. The GH13_20 neopullulanase from Bacillus stearothermophilus in complex with maltotetraose is a representative of the neopullulanase subfamily. One of the most clear sequence signatures of true members of the neopullulanase subfamily subfamily GH13_20 is the stretch VANE succeeding the catalytic nucleophile in the CSR II, although not every GH13_20 member has to possess this segment in its entirety. GH13_36 enzymes contain a histidine at the end of the CSR II (strand beta4) and a tryptophan (or at least an aromatic residue) two residues succeeding the catalytic proton donor in the CSR III (strand beta5)

731584

physiological function

2 entries

physiological function

Ruminococcus bromii

-

the enzyme may have a role to provide branched maltooligosaccharides to stimulate the growth of beneficial microorganisms in the human intestine

750815

physiological function

Ruminococcus bromii ATCC 27255

-

the enzyme may have a role to provide branched maltooligosaccharides to stimulate the growth of beneficial microorganisms in the human intestine

-