3.2.1.135: neopullulanase

This is an abbreviated version!
For detailed information about neopullulanase, go to the full flat file.

Reaction

Synonyms

Amo105, amylopullalanase, amylopullulanase, ApuA, ApuADELTA, bsNpl, cyclomaltodextrinase, Env Npu193A, More, neopullulanase-alpha-amylase, neopullulanase-like enzyme, Pul, pullulan 4-D-glucanohydrolase (6-alpha-D-glucosylmaltose), pullulan hydrolase type I, pullulanase, pullulanase II, pullulanase, neo-, Rbamy5, TetApuM955, TetApuR855, type II pullulanase, type III pullulan hydrolase

ECTree

     3 Hydrolases

         3.2 Glycosylases

             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds

                3.2.1.135 neopullulanase

Advanced search results

Do not include text mining results

Include results (more...)

Include results (more...)

Results	in table
1314	AA Sequence
5	Application
1	CAS Registry Number
14	Cloned(Commentary)
2	Crystallization (Commentary)
3	General Information
1	General Stability
19	Inhibitors
1	kcat/KM [mM/s]
12	KM Value [mM]
14	Localization
15	Metals/Ions
23	Molecular Weight [Da]
41	Natural Substrates/ Products (Substrates)
86	Organism
43	PDB ID
16	pH Optimum
6	pH Range
5	pH Stability
41	Protein Variants
11	Purification (Commentary)
5	Reaction
8	Reaction Type
65	Reference
2	Source Tissue
18	Specific Activity [micromol/min/mg]
1	Storage Stability
169	Substrates and Products (Substrate)
23	Subunits
60	Synonyms
1	Systematic Name
17	Temperature Optimum [°C]
5	Temperature Range [°C]
15	Temperature Stability [°C]
7	Turnover Number [1/s]

pH Stability

print visible entries

print all entries

Go back to the abbreviated version
of the Enzyme Summary Page.

pH Stability on EC 3.2.1.135 - neopullulanase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.

top print hide show all columns Go to pH Stability Search

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

pH STABILITY

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

2 - 8

2 entries

3 - 9

uncultured microorganism

A7LI67

substrate pullulan, 25°C, 4 h pre-incubation at pH 3: 50 mM sodium citrate, pH 5: 50 mM sodium acetate, pH 7: 50 mM Tris-HCl, pH 9: 50 mM glycin, more than 50% activity retained at pH 3, more than 80% at pH 5, about 100% at pH 7 and 9

696810

6

Paenibacillus polymyxa

-

30 min, 50°C, stable

646822

additional information

Nesterenkonia halobia

-

-

646821

2 - 8

Lactiplantibacillus plantarum

-

C-terminal truncated enzyme ApuADELTA, pre-incubation for 1 h, 40°C, 0.1 M buffers pH 2.5-8, 90-100% activity from pH 2.5-6.6, about 80% activity at pH 7, about 60% at pH 7.5, about 30% activity at pH 8

699051

2 - 8

Lactiplantibacillus plantarum

-

full length enzyme slightly less stable than C-terminal truncated enzyme ApuADELTA, pre-incubation for 1 h, 40°C, 0.1 M buffers pH 2.5-8, 90-100% activity from pH 2.5-6, about 80% activity at pH 6.5, about 50% at pH 7, between 20-30% activity at pH 7.5 and 8

699051