3.2.1.135: neopullulanase

This is an abbreviated version!
For detailed information about neopullulanase, go to the full flat file.

Reaction

Synonyms

Amo105, amylopullalanase, amylopullulanase, ApuA, ApuADELTA, bsNpl, cyclomaltodextrinase, Env Npu193A, More, neopullulanase-alpha-amylase, neopullulanase-like enzyme, Pul, pullulan 4-D-glucanohydrolase (6-alpha-D-glucosylmaltose), pullulan hydrolase type I, pullulanase, pullulanase II, pullulanase, neo-, Rbamy5, TetApuM955, TetApuR855, type II pullulanase, type III pullulan hydrolase

ECTree

     3 Hydrolases

         3.2 Glycosylases

             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds

                3.2.1.135 neopullulanase

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Results	in table
1314	AA Sequence
5	Application
1	CAS Registry Number
14	Cloned(Commentary)
2	Crystallization (Commentary)
3	General Information
1	General Stability
19	Inhibitors
1	kcat/KM [mM/s]
12	KM Value [mM]
14	Localization
15	Metals/Ions
23	Molecular Weight [Da]
41	Natural Substrates/ Products (Substrates)
86	Organism
43	PDB ID
16	pH Optimum
6	pH Range
5	pH Stability
41	Protein Variants
11	Purification (Commentary)
5	Reaction
8	Reaction Type
65	Reference
2	Source Tissue
18	Specific Activity [micromol/min/mg]
1	Storage Stability
169	Substrates and Products (Substrate)
23	Subunits
60	Synonyms
1	Systematic Name
17	Temperature Optimum [°C]
5	Temperature Range [°C]
15	Temperature Stability [°C]
7	Turnover Number [1/s]

pH Range

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pH Range on EC 3.2.1.135 - neopullulanase

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pH RANGE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

2 - 7

Lactiplantibacillus plantarum

-

40°C, 0.1 M buffers pH 2.5-4: glycine-HCl, pH 3.5-6: acetate, pH 6-8: phosphate, substrate: soluble starch

699051

4 - 11

uncultured microorganism

A7LI67

substrate pullulan: at least 50% activity retained between pH 5 and 9, about 20% activity at pH 4 and beyond pH 10. buffers of 100 mM pH 4: sodium citrate, pH 5-6: acetic acid, pH 6.5-7: MOPS, pH 7-9: Tris-HCl, pH 9-11: glycine

696810

5 - 7

2 entries

5 - 8

Paenibacillus polymyxa

-

pH 5 and 8: more than 50% activity

646822

6 - 9

Bacillus cereus

-

pH 6.0: about 60% of maximal activity, pH 9.0: about 50% of maximal activity

749679

5 - 7

Geobacillus stearothermophilus

-

-

703502

5 - 7

Geobacillus stearothermophilus

-

pH 5: about 25% of activity maximum, pH 7: about 40% of activity maximum

646810