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3.2.1.135: neopullulanase

This is an abbreviated version!
For detailed information about neopullulanase, go to the full flat file.

Word Map on EC 3.2.1.135

Reaction

pullulan
+
H2O
=
panose

Synonyms

Amo105, amylopullalanase, amylopullulanase, ApuA, ApuADELTA, bsNpl, cyclomaltodextrinase, Env Npu193A, More, neopullulanase-alpha-amylase, neopullulanase-like enzyme, Pul, pullulan 4-D-glucanohydrolase (6-alpha-D-glucosylmaltose), pullulan hydrolase type I, pullulanase, pullulanase II, pullulanase, neo-, Rbamy5, TetApuM955, TetApuR855, type II pullulanase, type III pullulan hydrolase

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.135 neopullulanase

Cloned

Cloned on EC 3.2.1.135 - neopullulanase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
all pul genes expressed in Escherichia coli K-12 strains, all pull genes except pulL expressed from plasmid pCHAP1217, pulL expressed from plasmid pCHAP710. Overexpression of gfp-pulL or gfp-pulM in Escherichia coli
-
Bifidobacterium breve cultured in reinforced clostridial medium, anaeorbic, 37°C. Escherichia coli cultured in Luria Bertani broth, agitated, 37°C. Lactococcus lactis cultured in M17 broth containing 0.5% glucose, 30°C for amplification of vectro pNZ8048 containing only alpha-amylase or pullulanase encoding domains, respectively. Selection of recombinant Escherichia coli with pORI19-apuB on agar containing erythromycin and 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside and isopropyl-beta-D-galactopyranoside
-
development of an an hydrotetracycline-inducible expression system for expression of a neopullulanase in Bacillus subtilis
-
Escherichia coli DH5alphaF' grown in Luria-Bertani broth, 37°C with plasmid pTZ57R/T, expression in yeast Pichia pastoris with recombinant plasmid pPICZ-BK193 in Yeast Extract Peptone Dextrose
Escherichia coli NovaBlue and Rosetta (DE3) pLysS, cloning and expression host, respectively, with plasmid pET-20b(+), in LB broth, 37°C
-
expression in Bacillus subtilis
-
expression in bacteria
-
expression in Escherichia coli
expression in Escherichia coli BL21 (DE3)
-
expression in Escherichia coli CodonPlus BL21 (DE3)
expression in Escherichia coli. A poly-His-tag placed at N-terminal region can induce over-expression of recombinant protein via subcellular uneven distribution in vivo in the cytoplasm of Escherichia coli
expression in Saccharomyces cerevisiae
NCL21 with plasmid pRN-PulSa, pRN-Puldelta3
-