3.2.1.135: neopullulanase
This is an abbreviated version!
For detailed information about neopullulanase, go to the full flat file.
Word Map on EC 3.2.1.135
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3.2.1.135
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starch
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stearothermophilus
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amylolytic
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alpha-amylases
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cyclomaltodextrinase
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maltogenic
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amylopectin
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transglycosylation
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amylose
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alpha-1,4
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amylopullulanase
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debranching
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oligo-1,6-glucosidase
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glucanotransferase
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thermoactinomyces
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alpha-1,6-glucosidic
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isopanose
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cdase
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saccharifying
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pullulanases
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cyclodextrinase
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maltooligosaccharides
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isoamylase
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isopullulanase
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gamma-cyclodextrins
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biotechnology
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industry
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synthesis
- 3.2.1.135
- starch
- stearothermophilus
-
amylolytic
- alpha-amylases
- cyclomaltodextrinase
-
maltogenic
- amylopectin
-
transglycosylation
- amylose
-
alpha-1,4
- amylopullulanase
-
debranching
- oligo-1,6-glucosidase
-
glucanotransferase
-
thermoactinomyces
-
alpha-1,6-glucosidic
- isopanose
-
cdase
-
saccharifying
- pullulanases
- cyclodextrinase
- maltooligosaccharides
- isoamylase
- isopullulanase
- gamma-cyclodextrins
- biotechnology
- industry
- synthesis
Reaction
Synonyms
Amo105, amylopullalanase, amylopullulanase, ApuA, ApuADELTA, bsNpl, cyclomaltodextrinase, Env Npu193A, More, neopullulanase-alpha-amylase, neopullulanase-like enzyme, Pul, pullulan 4-D-glucanohydrolase (6-alpha-D-glucosylmaltose), pullulan hydrolase type I, pullulanase, pullulanase II, pullulanase, neo-, Rbamy5, TetApuM955, TetApuR855, type II pullulanase, type III pullulan hydrolase
ECTree
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Cloned
Cloned on EC 3.2.1.135 - neopullulanase
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all pul genes expressed in Escherichia coli K-12 strains, all pull genes except pulL expressed from plasmid pCHAP1217, pulL expressed from plasmid pCHAP710. Overexpression of gfp-pulL or gfp-pulM in Escherichia coli
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Bifidobacterium breve cultured in reinforced clostridial medium, anaeorbic, 37°C. Escherichia coli cultured in Luria Bertani broth, agitated, 37°C. Lactococcus lactis cultured in M17 broth containing 0.5% glucose, 30°C for amplification of vectro pNZ8048 containing only alpha-amylase or pullulanase encoding domains, respectively. Selection of recombinant Escherichia coli with pORI19-apuB on agar containing erythromycin and 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside and isopropyl-beta-D-galactopyranoside
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development of an an hydrotetracycline-inducible expression system for expression of a neopullulanase in Bacillus subtilis
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Escherichia coli DH5alphaF' grown in Luria-Bertani broth, 37°C with plasmid pTZ57R/T, expression in yeast Pichia pastoris with recombinant plasmid pPICZ-BK193 in Yeast Extract Peptone Dextrose
Escherichia coli NovaBlue and Rosetta (DE3) pLysS, cloning and expression host, respectively, with plasmid pET-20b(+), in LB broth, 37°C
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expression in Escherichia coli
expression in Escherichia coli. A poly-His-tag placed at N-terminal region can induce over-expression of recombinant protein via subcellular uneven distribution in vivo in the cytoplasm of Escherichia coli