3.2.1.135: neopullulanase
This is an abbreviated version!
For detailed information about neopullulanase, go to the full flat file.
Word Map on EC 3.2.1.135
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3.2.1.135
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starch
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stearothermophilus
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amylolytic
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alpha-amylases
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cyclomaltodextrinase
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maltogenic
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amylopectin
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transglycosylation
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amylose
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alpha-1,4
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amylopullulanase
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debranching
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oligo-1,6-glucosidase
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glucanotransferase
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thermoactinomyces
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alpha-1,6-glucosidic
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isopanose
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cdase
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saccharifying
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pullulanases
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cyclodextrinase
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maltooligosaccharides
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isoamylase
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isopullulanase
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gamma-cyclodextrins
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biotechnology
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industry
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synthesis
- 3.2.1.135
- starch
- stearothermophilus
-
amylolytic
- alpha-amylases
- cyclomaltodextrinase
-
maltogenic
- amylopectin
-
transglycosylation
- amylose
-
alpha-1,4
- amylopullulanase
-
debranching
- oligo-1,6-glucosidase
-
glucanotransferase
-
thermoactinomyces
-
alpha-1,6-glucosidic
- isopanose
-
cdase
-
saccharifying
- pullulanases
- cyclodextrinase
- maltooligosaccharides
- isoamylase
- isopullulanase
- gamma-cyclodextrins
- biotechnology
- industry
- synthesis
Reaction
Synonyms
Amo105, amylopullalanase, amylopullulanase, ApuA, ApuADELTA, bsNpl, cyclomaltodextrinase, Env Npu193A, More, neopullulanase-alpha-amylase, neopullulanase-like enzyme, Pul, pullulan 4-D-glucanohydrolase (6-alpha-D-glucosylmaltose), pullulan hydrolase type I, pullulanase, pullulanase II, pullulanase, neo-, Rbamy5, TetApuM955, TetApuR855, type II pullulanase, type III pullulan hydrolase
ECTree
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Temperature Range
Temperature Range on EC 3.2.1.135 - neopullulanase
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20 - 60
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C-terminal truncated enzyme ApuADELTA with only slightly higher activities than full length enzyme, especially at higher than optimal temperatures, about 100% activity at 40-45°C, 60-90% activity at 20-35°C and 50°C, 20-40% activity at 55-60°C, 0.1 M sodium acetate butter, pH 4.5, starch as substrate
20 - 70
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20°C: about 45% of maximal activity, 70°C: about 65% of maximal activity
30 - 70
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30°C: about 70% of activity maximum, 70°C: about 50% of activity maximum
30 - 90
substrate pullulan: more than 80% activity from 65-80°C, 70-80% activity from 55-60°C, about 40% activity between 40 and 50°C, about 20-30% activity at 30°C and beyond 80°C
40 - 80
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40°C: about 50% of activity maximum, 80°C: about 25% of activity maximum