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3.2.1.135: neopullulanase

This is an abbreviated version!
For detailed information about neopullulanase, go to the full flat file.

Word Map on EC 3.2.1.135

Reaction

pullulan
+
H2O
=
panose

Synonyms

Amo105, amylopullalanase, amylopullulanase, ApuA, ApuADELTA, bsNpl, cyclomaltodextrinase, Env Npu193A, More, neopullulanase-alpha-amylase, neopullulanase-like enzyme, Pul, pullulan 4-D-glucanohydrolase (6-alpha-D-glucosylmaltose), pullulan hydrolase type I, pullulanase, pullulanase II, pullulanase, neo-, Rbamy5, TetApuM955, TetApuR855, type II pullulanase, type III pullulan hydrolase

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.135 neopullulanase

Temperature Range

Temperature Range on EC 3.2.1.135 - neopullulanase

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TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
20 - 60
-
C-terminal truncated enzyme ApuADELTA with only slightly higher activities than full length enzyme, especially at higher than optimal temperatures, about 100% activity at 40-45°C, 60-90% activity at 20-35°C and 50°C, 20-40% activity at 55-60°C, 0.1 M sodium acetate butter, pH 4.5, starch as substrate
20 - 70
-
20°C: about 45% of maximal activity, 70°C: about 65% of maximal activity
30 - 70
-
30°C: about 70% of activity maximum, 70°C: about 50% of activity maximum
30 - 90
substrate pullulan: more than 80% activity from 65-80°C, 70-80% activity from 55-60°C, about 40% activity between 40 and 50°C, about 20-30% activity at 30°C and beyond 80°C
40 - 80
-
40°C: about 50% of activity maximum, 80°C: about 25% of activity maximum