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3.2.1.135: neopullulanase

This is an abbreviated version!
For detailed information about neopullulanase, go to the full flat file.

Word Map on EC 3.2.1.135

Reaction

pullulan
+
H2O
=
panose

Synonyms

Amo105, amylopullalanase, amylopullulanase, ApuA, ApuADELTA, bsNpl, cyclomaltodextrinase, Env Npu193A, More, neopullulanase-alpha-amylase, neopullulanase-like enzyme, Pul, pullulan 4-D-glucanohydrolase (6-alpha-D-glucosylmaltose), pullulan hydrolase type I, pullulanase, pullulanase II, pullulanase, neo-, Rbamy5, TetApuM955, TetApuR855, type II pullulanase, type III pullulan hydrolase

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.135 neopullulanase

Molecular Weight

Molecular Weight on EC 3.2.1.135 - neopullulanase

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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
100000
-
1 * 100000, SDS-PAGE
107800
-
calculated mass of matured full length enzmye TetAbuM955, corroborated by SDS-PAGE, 13 N-terminal amino acids (signal peptide) cleaved off proteolytically, DNA fragment 2882 bp
107818
-
x * 107818, SDS-PAGE
175000
-
matured enzyme linked to peptidoglycan at carboxy terminus, premature 182360 Da product of 5127 bp apuB gene with 1708 amino acids, signal sequence of 34 amino acids with cleavage site located between Ala34 and Asp35, 4 highly conserved regions, calculated from sequence
53000
-
SDS-PAGE
58000
62000
65000
66000
70000
70156
enzyme likely to be present in monomer-dimer equilibrium with a molecular ratio of 1:9 in 50 mM sodium acetate buffer, pH 6.0, x * 65000, SDS-PAGE, x * 70156, MALDI-TOF MS analyzis
85000
-
theoretically calculated mass of truncated TetApuM855, corroborated by SDS-PAGE