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3.2.1.135: neopullulanase

This is an abbreviated version!
For detailed information about neopullulanase, go to the full flat file.

Word Map on EC 3.2.1.135

Reaction

pullulan
+
H2O
=
panose

Synonyms

Amo105, amylopullalanase, amylopullulanase, ApuA, ApuADELTA, bsNpl, cyclomaltodextrinase, Env Npu193A, More, neopullulanase-alpha-amylase, neopullulanase-like enzyme, Pul, pullulan 4-D-glucanohydrolase (6-alpha-D-glucosylmaltose), pullulan hydrolase type I, pullulanase, pullulanase II, pullulanase, neo-, Rbamy5, TetApuM955, TetApuR855, type II pullulanase, type III pullulan hydrolase

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.135 neopullulanase

Specific Activity

Specific Activity on EC 3.2.1.135 - neopullulanase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.00000431
-
full length enzyme
0.00000596
-
C-terminal truncated enzyme ApuADELTA
0.15
Vmax, substrate starch, 50 mM MOPS buffer, pH 7.0, 2 mM CaCl2, 75°C, 45 min
13.43
-
37°C, pH 7
16.6
-
maltotriose as substrate, wild-type enzyme, comparison with mutant enzymes
23.24
Vmax, substrate pullulan, 50 mM MOPS buffer, pH 7.0, 2 mM CaCl2, 75°C, 45 min
28.6
-
pullulan as substrate, wild-type enzyme, comparison with mutant enzymes
72.5
-
45°C, pH 5.0
additional information