General Stability | Organism |
---|---|
2 mM EDTA and 2 mM phytic acid decreases the stability of the enzyme | Geobacillus stearothermophilus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 5 mM Ca2+ increases the stability of the enzyme | Geobacillus stearothermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
100000 | - |
1 * 100000, SDS-PAGE | Geobacillus stearothermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | - |
- |
- |
Geobacillus stearothermophilus L14 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, DEAE-Sepharose column chromatography, and Sephadex G-100 gel filtration | Geobacillus stearothermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
maltoheptaose + H2O | - |
Geobacillus stearothermophilus | ? | - |
? | |
maltoheptaose + H2O | - |
Geobacillus stearothermophilus L14 | ? | - |
? | |
maltohexaose + H2O | - |
Geobacillus stearothermophilus | ? | - |
? | |
maltohexaose + H2O | - |
Geobacillus stearothermophilus L14 | ? | - |
? | |
maltopentaose + H2O | - |
Geobacillus stearothermophilus | ? | - |
? | |
maltopentaose + H2O | - |
Geobacillus stearothermophilus L14 | ? | - |
? | |
maltotetraose + H2O | - |
Geobacillus stearothermophilus | ? | - |
? | |
maltotetraose + H2O | - |
Geobacillus stearothermophilus L14 | ? | - |
? | |
maltotriose + H2O | - |
Geobacillus stearothermophilus | ? | - |
? | |
additional information | the enzyme is unable to degrade alpha-cyclodextrin and panose | Geobacillus stearothermophilus | ? | - |
? | |
additional information | the enzyme is unable to degrade alpha-cyclodextrin and panose | Geobacillus stearothermophilus L14 | ? | - |
? | |
pullulan + H2O | the enzyme attacks both alpha-D-(1->4) and alpha-D-(1->6) glycosidic linkages | Geobacillus stearothermophilus | D-glucose + maltose + maltotriose | - |
? | |
soluble starch + H2O | - |
Geobacillus stearothermophilus | D-glucose + maltose | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 100000, SDS-PAGE | Geobacillus stearothermophilus |
Synonyms | Comment | Organism |
---|---|---|
amylopullulanase | - |
Geobacillus stearothermophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
- |
Geobacillus stearothermophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | 70 | the irreversible thermoinactivation of the enzyme is recorded in 50 mM sodium acetate, pH 5.5 at 60, 70 and 80°C. Pullulanase retains 85% of its activity after 50 min of incubation at 60°C, its residual activities at 70 and 80°C after 50 min are 70 and 15%, respectively | Geobacillus stearothermophilus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
62000 | - |
pullulan | - |
Geobacillus stearothermophilus | |
111000 | - |
soluble starch | - |
Geobacillus stearothermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
- |
Geobacillus stearothermophilus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 7 | - |
Geobacillus stearothermophilus |