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3.2.1.113: mannosyl-oligosaccharide 1,2-alpha-mannosidase

This is an abbreviated version!
For detailed information about mannosyl-oligosaccharide 1,2-alpha-mannosidase, go to the full flat file.

Word Map on EC 3.2.1.113

Reaction

Man6GlcNAc2-[protein] (isomer 6A1,2,3)
+
H2O
=
Man5GlcNAc2-[protein]
 +
beta-D-mannopyranose

Synonyms

(alpha1,2)-mannosidase-I, 1,2-alpha-mannosidase, alpha-(1->2)-mannosidase I, alpha-1,2 mannosidase I, alpha-1,2-mannosidase, alpha-1,2-mannosidase IC, alpha-1,2-mannosidaseI, alpha-1-2-mannosidase, alpha-mannosidase, alpha1,2-mannosidase, alpha1,2-mannosidase I, alpha1-2-mannosidase I, At1g51590, At3g21160, class I endoplasmic reticulum 1,2-alpha-mannosidase, D2030.1, endoplasmic reticulum mannosidase I, ER alpha-1,2-mannosidase, ER-Man, exo-alpha-1,2-mannanase, FmanIBp, fungal alpha-1,2-mannosidase, glycoprotein processing mannosidase I, Golgi alpha-mannosidase I, Golgi alpha1,2-mannosidase I, Golgi alpha1,2-mannosidase IA, Golgi alpha1,2-mannosidase IB, Golgi alpha1,2-mannosidase IC, Golgi alpha1,2-mannosidase II, GolgiManI, HMIC, Man(9)-alpha-mannosidase, MAN1A1, MAN1A2, MAN1B1, MAN1C1, Man9 processing alpha-mannosidase, Man9-alpha-mannosidase, Man9-mannosidase, Man9GlcNAc2-specific processing alpha-mannosidase, manE, ManI, MANIa, ManIA2, MANIb, ManIC, mannose-9 processing alpha-mannosidase, mannosidase 1A, mannosidase 1B, mannosidase I, mannosidase, exo-1,2-alpha-, Mas1, MIa, MIa,b,c, MIb, MIc, MII, MIIx, MNS1, Mns1p, MNS2, msdC, N-glycan processing class I alpha-1,2-mannosidase, processing alpha-1,2-mannosidase IC

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.113 mannosyl-oligosaccharide 1,2-alpha-mannosidase

Substrates Products

Substrates Products on EC 3.2.1.113 - mannosyl-oligosaccharide 1,2-alpha-mannosidase

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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(Glc)1(Man)9(GlcNAc)2 + H2O
? + D-mannose
show the reaction diagram
-
removal of one alpha1,2-linked mannose
-
-
?
(Glc)3(Man)9(GlcNAc)2 + H2O
? + D-mannose
show the reaction diagram
-
removal of one alpha1,2-linked mannose
-
-
?
(Manalpha(1-2))nManalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-3)) Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
Manalpha1-6(Manalpha1-3)Manalpha1-6(Manalpha1-3)Manbeta1-4GlcNAc beta1-4GlcNAc + D-mannose
show the reaction diagram
-
-
-
?
1,2-alpha-D-mannobiose + H2O
D-mannose
show the reaction diagram
-
-
-
-
?
1,2-alpha-mannobiose + H2O
D-mannose
show the reaction diagram
-
-
-
-
?
4-methylumbelliferyl alpha-D-mannopyranoside + H2O
4-methylumbelliferone + alpha-D-mannopyranose
show the reaction diagram
alpha-1,2-linked mannotetraose + H2O
? + D-mannose
show the reaction diagram
alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->6)]-alpha-D-Man-(1->6)]-alpha-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[protein] + 4 H2O
alpha-D-Man-(1->3)-[alpha-D-Man-(1->3)-[alpha-D-Man-(1->6)]-alpha-D-Man-(1->6)]-alpha-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[protein] + 4 beta-D-mannopyranose
show the reaction diagram
alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,6)]-alpha-D-mannopyranosyl-(1,6)]-beta-D-mannopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranose + 4 H2O
alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,6)]-alpha-D-mannopyranosyl-(1,6)]-beta-D-mannopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranose + 4 D-mannose
show the reaction diagram
alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,6)]-alpha-D-mannopyranosyl-(1,6)]-beta-D-mannopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranose + H2O
alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,6)]-alpha-D-mannopyranosyl-(1,6)]-beta-D-mannopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranose + D-mannose
show the reaction diagram
-
-
-
?
baker's yeast mannan + H2O
? + D-mannose
show the reaction diagram
Glc3Man9GlcNAc2 from oligosaccharide-lipid + H2O
?
show the reaction diagram
-
good substrate
-
-
?
GlcNAc-(Man)5GlcNAc + H2O
? + D-mannose
show the reaction diagram
-
poor substrate
-
-
?
lipid-linked Man7GlcNAc2 (isomer 7A1,3B1,3) + H2O
?
show the reaction diagram
-
10% activity compared to Man9GlcNAc2
-
-
?
Man6GlcNAc + H2O
Man5GlcNAc + D-mannose
show the reaction diagram
-
-
-
-
?
Man8GlcNAc + H2O
Man7GlcNAc + D-mannose
show the reaction diagram
-
-
-
-
?
Man8GlcNAc2 + H2O
Man5GlcNAc2 + D-mannose
show the reaction diagram
-
-
-
-
?
Man9GlcNAc2 + 4 H2O
Man5GlcNAc2 + 4 D-mannose
show the reaction diagram
-
-
-
-
?
Man9GlcNAc2 + H2O
Man8GlcNAc2 (isomer 8A1,2,3B1,3) + D-mannose
show the reaction diagram
Man9GlcNAc2-Asn + 4 H2O
Man5GlcNAc2-Asn + 4 beta-D-mannose
show the reaction diagram
Man9GlcNAc2-Asn + H2O
Man8GlcNAc2-Asn (isomer 8A1,2,3B1,3) + D-mannose
show the reaction diagram
Man9GlcNAc2-pyridylamine + H2O
Man8GlcNAc2-pyridylamine (isomer 8A1,2,3B1,3) + D-mannose
show the reaction diagram
Man9GlcNAc2-[thyroglobulin] + H2O
Man8GlcNAc2-[thyroglobulin] + D-mannose
show the reaction diagram
-
good substrate
-
-
?
Manalpha(1,2)Man-O-Me + H2O
?
show the reaction diagram
-
-
-
-
?
Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)[Manalpha(1-2)Manalpha(1-3)[Manalpha(1-2)Manalpha(1-6)]Manalpha(1-6)]Manbeta(1-4)GlcNACbeta(1-4)GlcNAc + H2O
Manalpha(1-3)[Manalpha(1-3)[Manalpha(1-6)]Manalpha(1-6)]Manbeta(1-4)GlcNACbeta(1-4)GlcNAc + 4 D-mannose
show the reaction diagram
-
-
-
-
?
Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)[Manalpha(1-2)Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-6)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
Manalpha(1-3)[Manalpha(1-3)[Manalpha(1-6)]Manalpha(1-6)]Manbeta(1-4)GlcNACbeta(1-4)GlcNAc + 3 D-mannose
show the reaction diagram
Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + H2O
? + D-mannose
show the reaction diagram
-
-
-
-
?
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + 4 H2O
(Man)5GlcNAc + 4 D-mannose
show the reaction diagram
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
(Man)6(GlcNAc)2 + D-mannose
show the reaction diagram
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-hexapeptide + H2O
(Man)6(GlcNAc)2-hexapeptide + D-mannose
show the reaction diagram
-
substrate specificity not influenced by peptide moiety, removes 3 mannose residues
-
?
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-2)Manalpha(1-2))Manalpha(1-3)Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-peptide + H2O
(Man)5(GlcNAc)-peptide + D-mannose
show the reaction diagram
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-2)Manalpha(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAc + H2O
? + D-mannose
show the reaction diagram
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-2)Manalpha(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
? + D-mannose
show the reaction diagram
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manbeta(1-4)GlcNA + H2O
? + D-mannose
show the reaction diagram
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
? + D-mannose
show the reaction diagram
Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAc + H2O
(Man)5(GlcNAc)2 + D-mannose
show the reaction diagram
Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
(Man)5(GlcNAc)2-Asn + D-mannose
show the reaction diagram
-
i.e. GPIV
major product formed, 3.5 mol mannose/mol GP IV per h, i.e. GPI
?
Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-Asn + H2O
? + D-mannose
show the reaction diagram
Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-3))Manbeta(1-4)GlcNAc + H2O
(Man)4GlcNAc + (Man)3GlcNAc + D-mannose
show the reaction diagram
Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
(Man)4(GlcNAc)2 + (Man)3(GlcNAc)2 + D-mannose
show the reaction diagram
Manalpha(1->2)Manalpha(1->2)Manalpha(1->3)[Manalpha(1->2)Manalpha(1->6)[Manalpha(1->2)Manalpha(1->3)]Manalpha(1->6)]Manbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta-2-pyridylamine + H2O
Manalpha(1->2)Manalpha(1->3)[Manalpha(1->2)Manalpha(1->6)[Manalpha(1->2)Manalpha(1->3)]Manalpha(1->6)]Manbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta-2-pyridylamine + D-mannose
show the reaction diagram
-
-
-
?
Manalpha(1->2)Manalpha(1->3)[Manalpha(1->2)Manalpha(1->6)[Manalpha(1->2)Manalpha(1->3)]Manalpha(1->6)]Manbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta-2-pyridylamine + H2O
Manalpha(1->3)[Manalpha(1->2)Manalpha(1->6)[Manalpha(1->2)Manalpha(1->3)]Manalpha(1->6)]Manbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta-2-pyridylamine + D-mannose
show the reaction diagram
-
-
-
?
Manalpha(1->3)[Manalpha(1->2)Manalpha(1->6)[Manalpha(1->2)Manalpha(1->3)]Manalpha(1->6)]Manbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta-2-pyridylamine + H2O
Manalpha(1->3)[Manalpha(1->6)[Manalpha(1->2)Manalpha(1->3)]Manalpha(1->6)]Manbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta-2-pyridylamine + D-mannose
show the reaction diagram
-
-
-
?
Manalpha(1->3)[Manalpha(1->6)[Manalpha(1->2)Manalpha(1->3)]Manalpha(1->6)]Manbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta-2-pyridylamine + H2O
Manalpha(1->3)[Manalpha(1->6)[Manalpha(1->3)]Manalpha(1->6)]Manbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta-2-pyridylamine + D-mannose
show the reaction diagram
-
-
-
?
mannobiose + H2O
2 D-mannose
show the reaction diagram
-
-
-
-
?
methyl 2-O-alpha-D-mannopyranosyl-alpha-D-mannopyranoside
? + D-mannose
show the reaction diagram
-
-
-
?
methyl 2-O-alpha-D-mannopyranosyl-alpha-D-mannopyranoside + H2O
? + D-mannose
show the reaction diagram
used in a spectrophotometric assay
-
-
?
ovalbumin glycopeptide + H2O
? + D-mannose
show the reaction diagram
-
-
-
-
?
p-nitrophenyl-alpha-D-mannoside + H2O
4-nitrophenol + D-mannose
show the reaction diagram
pyridylamino derivative of (Man)9(GlcNAc)2 + H2O
pyridylamino derivative of (Man)5(GlcNAc)2 + D-mannose
show the reaction diagram
-
-
after 120 h incubation, via specific intermediates: overview
?
[alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->6)]-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc]-N-Asn-[protein] + H2O
[alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->3)-[alpha-D-Man-(1->6)]-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc]-N-Asn-[protein] + D-mannose
show the reaction diagram
i.e. isomer 8A1,2,3B1,3
-
-
?
additional information
?
-