Information on EC 3.2.1.113 - mannosyl-oligosaccharide 1,2-alpha-mannosidase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
3.2.1.113
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RECOMMENDED NAME
GeneOntology No.
mannosyl-oligosaccharide 1,2-alpha-mannosidase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
Hydrolysis of the terminal (1->2)-linked alpha-D-mannose residues in the oligo-mannose oligosaccharide Man9(GlcNAc)2
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of O-glycosyl bond
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Metabolic pathways
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N-Glycan biosynthesis
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Various types of N-glycan biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
2-alpha-mannosyl-oligosaccharide alpha-D-mannohydrolase
Involved in the synthesis of glycoproteins.
CAS REGISTRY NUMBER
COMMENTARY hide
9068-25-1
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
M-90
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Manually annotated by BRENDA team
M-90
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Manually annotated by BRENDA team
calf
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Manually annotated by BRENDA team
wild-type, N2 strain
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Manually annotated by BRENDA team
wild-type, N2 strain
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Manually annotated by BRENDA team
Candida albicans ATCC26555
strain ATCC26555, CAI4, SC5314, NGY152, HMY5, HMY6
Uniprot
Manually annotated by BRENDA team
very similar (> 60%) to other fungal 1,2-alpha-mannosidases; silveira strain, formerly known as the non-California Coccidioides immitis
UniProt
Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Uniprot
Manually annotated by BRENDA team
strain Guy11
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Manually annotated by BRENDA team
strain Guy11
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
Rattus norvegicus Sprague-Dawley
Sprague-Dawley
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Manually annotated by BRENDA team
Wistar
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Manually annotated by BRENDA team
castor bean
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Manually annotated by BRENDA team
IPLB-SF-21AE, baculovirus-infected
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
intracellular protozoan, causes Chagas disease in humans, epimastigote stage
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Manually annotated by BRENDA team
two isoforms
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
metabolism
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part of N-glycan pathway
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(Glc)1(Man)9(GlcNAc)2 + H2O
? + D-mannose
show the reaction diagram
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removal of one alpha1,2-linked mannose
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(Glc)3(Man)9(GlcNAc)2 + H2O
? + D-mannose
show the reaction diagram
-
removal of one alpha1,2-linked mannose
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(Manalpha(1-2))nManalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-3)) Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
Manalpha1-6(Manalpha1-3)Manalpha1-6(Manalpha1-3)Manbeta1-4GlcNAc beta1-4GlcNAc + D-mannose
show the reaction diagram
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-
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?
1,2-alpha-D-mannobiose + H2O
D-mannose
show the reaction diagram
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-
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?
1,2-alpha-mannobiose + H2O
D-mannose
show the reaction diagram
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-
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?
2-O-alpha-D-mannopyranosyl-alpha-D-mannopyranose + H2O
2 alpha-D-mannose
show the reaction diagram
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?
2-O-alpha-D-mannopyranosyl-D-mannose + H2O
D-mannose
show the reaction diagram
4-methylumbelliferyl alpha-D-mannopyranoside + H2O
4-methylumbelliferone + alpha-D-mannopyranoside
show the reaction diagram
4-methylumbelliferyl-alpha-D-mannopyranoside
4-methylumbelliferone + D-mannose
show the reaction diagram
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MUaMan, fluorogenic substrate
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?
4-methylumbelliferyl-alpha-D-mannopyranoside + H2O
4-methylumbelliferone + alpha-D-mannose
show the reaction diagram
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-
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?
4-methylumbelliferyl-alpha-D-mannopyranoside + H2O
4-methylumbelliferone + D-mannopyranose
show the reaction diagram
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-
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?
4-methylumbelliferyl-alpha-D-mannopyranoside + H2O
4-methylumbelliferone + D-mannose
show the reaction diagram
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MUaMan fluorogenic substrate to measure enzyme activity
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?
4-nitrophenyl alpha-D-mannopyranoside + H2O
4-nitrophenol + alpha-D-mannopyranoside
show the reaction diagram
alpha-1,2-linked mannotetraose + H2O
? + D-mannose
show the reaction diagram
alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,6)]-alpha-D-mannopyranosyl-(1,6)]-beta-D-mannopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranose + H2O
? + D-mannose
show the reaction diagram
-
to measure enzyme activity
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?
alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,6)]-alpha-D-mannopyranosyl-(1,6)]-beta-D-mannopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranose + H2O
alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,6)]-alpha-D-mannopyranosyl-(1,6)]-beta-D-mannopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranose + D-mannose
show the reaction diagram
alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,6)]-alpha-D-mannopyranosyl-(1,6)]-beta-D-mannopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranose + H2O
alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,6)]-alpha-D-mannopyranosyl-(1,6)]-beta-D-mannopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranose + D-mannose
show the reaction diagram
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-
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?
alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,6)]-alpha-D-mannopyranosyl-(1,6)]-beta-D-mannopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranose + H2O
alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,6)]-alpha-D-mannopyranosyl-(1,6)]-beta-D-mannopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranose + D-mannose
show the reaction diagram
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?
alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,6)]-alpha-D-mannopyranosyl-(1,6)]-beta-D-mannopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranose + H2O
alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,6)]-alpha-D-mannopyranosyl-(1,6)]-beta-D-mannopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranose + D-mannose
show the reaction diagram
alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,6)]-alpha-D-mannopyranosyl-(1,6)]-beta-D-mannopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranose + H2O
alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,6)]-alpha-D-mannopyranosyl-(1,6)]-beta-D-mannopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranose + D-mannose
show the reaction diagram
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-
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?
alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,6)]-alpha-D-mannopyranosyl-(1,6)]-beta-D-mannopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranose + H2O
? + D-mannose
show the reaction diagram
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to measure enzyme activity
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?
alpha-D-Manp-(1-2)-D-Manp + H2O
D-mannose + ?
show the reaction diagram
alpha1-antitrypsin null (Hong Kong) + H2O
? + D-mannose
show the reaction diagram
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-
-
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?
alpha1-antitrypsin null + H2O
? + D-mannose
show the reaction diagram
alpha1-antitrypsin null + H2O
alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,6)]-alpha-D-mannopyranosyl-(1,6)]-beta-D-mannopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranose + D-mannose
show the reaction diagram
alpha1-antitrypsin null + H2O
alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,6)]-alpha-D-mannopyranosyl-(1,6)]-beta-D-mannopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranose + D-mannose
show the reaction diagram
alpha1-antitrypsin null + H2O
alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,6)]-alpha-D-mannopyranosyl-(1,6)]-beta-D-mannopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranose + D-mannose
show the reaction diagram
alpha1-antitrypsin null + H2O
alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,6)]-alpha-D-mannopyranosyl-(1,6)]-beta-D-mannopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranose + D-mannose
show the reaction diagram
alpha1-antitrypsin null + H2O
alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,6)]-alpha-D-mannopyranosyl-(1,6)]-beta-D-mannopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranose + D-mannose
show the reaction diagram
baker's yeast mannan + H2O
? + D-mannose
show the reaction diagram
Glcalpha(1-3)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)[Manalpha(1-2)Manalpha(1-3)[Manalpha(1-2)Manalpha(1-6)]Manalpha(1-6)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
Glcalpha(1-3)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)[Manalpha(1-2)Manalpha(1-3)[Manalpha(1-6)]Manalpha(1-6)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + D-mannose
show the reaction diagram
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-
-
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GlcNAc-(Man)5GlcNAc + H2O
? + D-mannose
show the reaction diagram
-
poor substrate
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-
?
high mannose chains of thyroglobulin and phytohemagglutinin-P + H2O
? + D-mannose
show the reaction diagram
-
thyroglobulin: 70% of alpha1,2-mannose residues accessible
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-
Man-alpha-1,2-Man-alpha-O-CH3 + H2O
methyl alpha-D-mannopyranoside + D-mannose
show the reaction diagram
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-
-
-
-
Man8GlcNAc2 + H2O
Man5GlcNAc2 + D-mannose
show the reaction diagram
-
-
-
-
?
Man8GlcNAc2 + H2O
Man7GlcNAc2 + D-mannose
show the reaction diagram
-
-
-
-
?
Man9GlcNAc2 + H2O
Man5GlcNAc2 + D-mannose
show the reaction diagram
-
-
-
-
?
Manalpha(1,2)Man-O-Me + H2O
?
show the reaction diagram
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-
-
-
?
Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)[Manalpha(1-2)Manalpha(1-3)[Manalpha(1-2)Manalpha(1-6)]Manalpha(1-6)]Manbeta(1-4)GlcNACbeta(1-4)GlcNAc + H2O
Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)[Manalpha(1-2)Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-6)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + D-mannose
show the reaction diagram
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the reaction is performed by ER-type alpha-mannosidase I protein MNS3
-
-
?
Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)[Manalpha(1-2)Manalpha(1-3)[Manalpha(1-2)Manalpha(1-6)]Manalpha(1-6)]Manbeta(1-4)GlcNACbeta(1-4)GlcNAc + H2O
Manalpha(1-3)[Manalpha(1-3)[Manalpha(1-6)]Manalpha(1-6)]Manbeta(1-4)GlcNACbeta(1-4)GlcNAc + 4 D-mannose
show the reaction diagram
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-
-
-
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Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)[Manalpha(1-2)Manalpha(1-6)[Manalpha(1-2)Manalpha(1-3)]Manalpha(1-6)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)[Manalpha(1-2)Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-6)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + D-mannose
show the reaction diagram
-
-
-
?
Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)[Manalpha(1-2)Manalpha(1-6)[Manalpha(1-2)Manalpha(1-3)]Manalpha(1-6)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)[Manalpha(1-6)[Manalpha(1-2)Manalpha(1-3)]Manalpha(1-6)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + alpha-D-mannose
show the reaction diagram
-
-
-
-
?
Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)[Manalpha(1-2)Manalpha(1-6)[Manalpha(1-2)Manalpha(1-3)]Manalpha(1-6)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)[Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-6)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + 2 alpha-D-mannose
show the reaction diagram
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-
-
-
?
Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)[Manalpha(1-2)Manalpha(1-6)[Manalpha(1-2)Manalpha(1-3)]Manalpha(1-6)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
Manalpha(1-2)Manalpha(1-3)[Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-6)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + 3 alpha-D-mannose
show the reaction diagram
-
-
-
-
?
Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)[Manalpha(1-2)Manalpha(1-6)[Manalpha(1-2)Manalpha(1-3)]Manalpha(1-6)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
Manalpha(1-3)[Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-6)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + 4 alpha-D-mannose
show the reaction diagram
-
-
-
-
?
Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)[Manalpha(1-2)Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-6)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)[Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-6)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + D-mannose
show the reaction diagram
cytosolic isoform E-I
-
-
?
Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)[Manalpha(1-2)Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-6)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
Manalpha(1-2)Manalpha(1-3)[Manalpha(1-2)Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-6)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + D-mannose
show the reaction diagram
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-
-
-
-
Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)[Manalpha(1-2)Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-6)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
Manalpha(1-3)[Manalpha(1-3)[Manalpha(1-6)]Manalpha(1-6)]Manbeta(1-4)GlcNACbeta(1-4)GlcNAc + 3 D-mannose
show the reaction diagram
Manalpha(1-2)Manalpha(1-3)Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + 2 H2O
(Man)5GlcNAc + 2 D-mannose
show the reaction diagram
-
-
-
-
?
Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + H2O
? + D-mannose
show the reaction diagram
-
-
-
-
?
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + 4 H2O
(Man)5GlcNAc + 4 D-mannose
show the reaction diagram
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
(Man)6(GlcNAc)2 + D-mannose
show the reaction diagram
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-hexapeptide + H2O
(Man)6(GlcNAc)2-hexapeptide + D-mannose
show the reaction diagram
-
substrate specificity not influenced by peptide moiety, removes 3 mannose residues
-
?
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-2)Manalpha(1-2))Manalpha(1-3)Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-peptide + H2O
(Man)5(GlcNAc)-peptide + D-mannose
show the reaction diagram
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-2)Manalpha(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAc + H2O
? + D-mannose
show the reaction diagram
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-2)Manalpha(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
? + D-mannose
show the reaction diagram
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manbeta(1-4)GlcNA + H2O
? + D-mannose
show the reaction diagram
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
? + D-mannose
show the reaction diagram
Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAc + H2O
(Man)5(GlcNAc)2 + D-mannose
show the reaction diagram
Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
(Man)5(GlcNAc)2-Asn + D-mannose
show the reaction diagram
Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-Asn + H2O
? + D-mannose
show the reaction diagram
Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-3))Manbeta(1-4)GlcNAc + H2O
(Man)4GlcNAc + (Man)3GlcNAc + D-mannose
show the reaction diagram
Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
(Man)4(GlcNAc)2 + (Man)3(GlcNAc)2 + D-mannose
show the reaction diagram
Manalpha(1->2)Manalpha(1->2)Manalpha(1->3)[Manalpha(1->2)Manalpha(1->6)[Manalpha(1->2)Manalpha(1->3)]Manalpha(1->6)]Manbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta-2-pyridylamine + H2O
Manalpha(1->2)Manalpha(1->3)[Manalpha(1->2)Manalpha(1->6)[Manalpha(1->2)Manalpha(1->3)]Manalpha(1->6)]Manbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta-2-pyridylamine + D-mannose
show the reaction diagram
Manalpha(1->2)Manalpha(1->3)[Manalpha(1->2)Manalpha(1->6)[Manalpha(1->2)Manalpha(1->3)]Manalpha(1->6)]Manbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta-2-pyridylamine + H2O
Manalpha(1->3)[Manalpha(1->2)Manalpha(1->6)[Manalpha(1->2)Manalpha(1->3)]Manalpha(1->6)]Manbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta-2-pyridylamine + D-mannose
show the reaction diagram
-
-
-
?
Manalpha(1->3)[Manalpha(1->2)Manalpha(1->6)[Manalpha(1->2)Manalpha(1->3)]Manalpha(1->6)]Manbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta-2-pyridylamine + H2O
Manalpha(1->3)[Manalpha(1->6)[Manalpha(1->2)Manalpha(1->3)]Manalpha(1->6)]Manbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta-2-pyridylamine + D-mannose
show the reaction diagram
-
-
-
?
Manalpha(1->3)[Manalpha(1->6)[Manalpha(1->2)Manalpha(1->3)]Manalpha(1->6)]Manbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta-2-pyridylamine + H2O
Manalpha(1->3)[Manalpha(1->6)[Manalpha(1->3)]Manalpha(1->6)]Manbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta-2-pyridylamine + D-mannose
show the reaction diagram
-
-
-
?
Manalpha(1->3)[Manalpha(1->6)[Manalpha(1->2)Manalpha(1->3)]Manalpha(1->6)]Manbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta-Asn + H2O
Manalpha(1->3)[Manalpha(1->6)[Manalpha(1->3)]Manalpha(1->6)]Manbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta + D-mannose
show the reaction diagram
methyl 2-O-alpha-D-mannopyranosyl-alpha-D-mannopyranoside + H2O
? + D-mannose
show the reaction diagram
used in a spectrophotometric assay
-
-
?
methyl-2-O-alpha-D-mannopyranosyl-alpha-D-mannopyranoside + H2O
? + D-mannose
show the reaction diagram
-
-
-
-
?
ovalbumin glycopeptide + H2O
? + D-mannose
show the reaction diagram
p-nitrophenyl-alpha-D-mannoside + H2O
4-nitrophenol + D-mannose
show the reaction diagram
pyridylamino derivative of (Man)9(GlcNAc)2 + H2O
pyridylamino derivative of (Man)5(GlcNAc)2 + D-mannose
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,6)]-alpha-D-mannopyranosyl-(1,6)]-beta-D-mannopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranose + H2O
alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,6)]-alpha-D-mannopyranosyl-(1,6)]-beta-D-mannopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranose + D-mannose
show the reaction diagram
alpha1-antitrypsin null + H2O
? + D-mannose
show the reaction diagram
Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)[Manalpha(1-2)Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-6)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)[Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-6)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + D-mannose
show the reaction diagram
Q8J0Q0
cytosolic isoform E-I
-
-
?
Manalpha(1-2)Manalpha(1-3)Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + 2 H2O
(Man)5GlcNAc + 2 D-mannose
show the reaction diagram
-
-
-
-
?
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + 4 H2O
(Man)5GlcNAc + 4 D-mannose
show the reaction diagram
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Cd2+
-
activation, 1 mM, inhibits at 10 mM
Cu2+
-
slight activation
Divalent cations
Fe2+
-
slight activation
Ni2+
-
activation
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,4-Dideoxy-1,4-imino-D-mannitol
1-Cyclohexyl-3-(2-morpholinyl-4-ethyl)carbodiimide/glycine methylester
-
-
1-deoxymannojirimycin
2-O-alpha-D-mannopyranosyl-alpha-D-mannopyranoside
-
in decreasing order of efficiency: alpha1,2, alpha 1,3 and alpha1,6-mannosylmannose
3-O-alpha-D-mannopyranosyl-alpha-D-mannopyranoside
-
in decreasing order of efficiency: alpha1,2, alpha 1,3 and alpha1,6-mannosylmannose
-
6-O-alpha-D-mannopyranosyl-alpha-D-mannopyranoside
-
in decreasing order of efficiency: alpha1,2, alpha 1,3 and alpha1,6-mannosylmannose
alpha1,2-Linked oligosaccharides
-
-
alpha1,3-Linked oligosaccharides
-
-
Ba2+
-
10 mM
BAPTA
-
i.e. 1,2-bis(2-aminophenoxy) ethane N,N,N',N'-tetraacetic acid
Basic sugar analogs of mannose
-
strong
-
brefeldin A
-
known inhibitor of vesicle transport, ablated intracellular turnover of the radiolabeled molecules. Plus digestion with potato acid phosphatase implies that phosphorylation of ER mannosidase I is responsible for the altered electrophoretic mobility
Chloroquine
cycloheximide
-
inhibitory effect like kifunensine
D-mannono-delta-lactam
-
-
D-mannono-gamma-lactone
D-Mannonolactam amidrazone
-
mannosidase inhibitor, MDCK-cells: in vivo and in vitro
deoxymannojirimycin
deoxymannojirimycin hydrochloride
inhibitor at a final concentration of 0.5 mM, reduces the activity by 80%
dithiothreitol
-
loss of activity with first-order kinetics
iodoacetic acid
kifunensine
lactacystin
Leupeptin
mannosamine
Mannosyl-mannose disaccharide
-
in decreasing order of efficiency: alpha1,2, alpha 1,3 and alpha1,6-mannosylmannose
-
methyl-alpha-D-lyxopyranosyl-(1',2)-alpha-D-mannopyranoside
analogue of the minimal disaccharide substrate mannobiose. Cocrystallization and mass spectrometry data suggest that when methyl-alpha-D-lyxopyranosyl-(1',2)-alpha-D-mannopyranoside is incubated with the mannosidase for long periods of time it is cleaved and that methyl-alpha-D-lyxopyranosyl-(1',2)-alpha-D-mannopyranoside behaves as a substrate
methyl-alpha-mannoside
N-5-Carboxypentyldeoxymannojirimycin
N-carbobenzoxyl-leucinyl-leucinyl-leucinal
-
degradation is inhibited by the proteasomal inhibitor
N-Methyl-deoxymannojirimycin
Na+
-
1 mM, weak, not at 10 mM
Ni2+
-
-
p-Chloromercuriphenylsulfonic acid
-
-
pepstatin A
-
in the presence of mixed membrane fraction from strain ATTC 26555, the 52 kDa polypeptide is converted into a 43 kDa active product. Protease inhibitors, such as phenylmethylsulphonylfluoride, trans-epoxysuccinyl-L-leucylamido(4-guanidino)butane and 1,10-phenantroline, fail to prevent proteolysis. Pepstatin A, an aspartyl protease inhibitor, fully blocked enzyme processing
phosphatidylinositol
-
CaCl2 or NaCl restores
Sn2+
-
strong
swainsonine
Ti4+
-
strong
Tris/maleate
-
-
additional information
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
phosphatidylcholine
-
activation, with acyl-chains of different lengths, above C-4
phosphatidylethanolamine
-
activation, in the presence of 3.5 mM Triton X-100
Triton X-100
Zwitterionic phospholipids
-
activation, required for solubilized and purified enzyme, independent of acyl chain length or degree of saturation, an ordered lipid structure of either micelles or bilayers, mixed micelles together with Triton X-100
-
additional information
-
no activation by negatively charged phospholipids, dibutyryl-phosphocholine
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.26
(Man)6GlcNAc
-
40C, pH 5.5
0.1
(Man)8GlcNAc
-
37C, pH 6.5
0.3
(Man)9GlcNAc
0.57
2-O-alpha-D-Mannopyranosyl-D-mannose
-
37C, pH 5.3
0.04
4-methylumbelliferyl alpha-D-mannopyranoside
-
at pH 7.0 in 50 mM MES-Tris buffer and at 37C
0.07 - 0.11
4-methylumbelliferyl-alpha-D-mannopyranoside
0.55
glycoprotein IV from ovalbumin
-
pH 6.0
-
0.296 - 0.78
Manalpha(1,2)Man-O-Me
0.015 - 0.51
Manalpha(1-2)Manalpha(1-3)Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc
1.25
Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc
-
-
0.068 - 0.26
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc
0.67
mannobiose
-
pH 5.0
0.6
methyl-mannopyranosyl-mannopyranoside
-
37C, pH 6.0
-
0.087
p-nitrophenyl-alpha-D-mannoside
-
37C, pH 6.1
additional information
additional information
-
kinetic constants of various substrate derivatives
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
3.87 - 9.48
Manalpha(1,2)Man-O-Me
0.03 - 3.7
Manalpha(1-2)Manalpha(1-3)Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc
0.000026 - 3.7
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.002
D-mannono-delta-lactam
-
pH 6.1, 37C
0.006
kifunensine
-
pH 6.1, 37C
0.6
methyl-alpha-D-lyxopyranosyl-(1',2)-alpha-D-mannopyranoside
-
0.0001
swainsonine
-
pH 6.1, 37C
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.019 - 0.23
1-deoxymannojirimycin
0.2
1-deoxymmanojirimycin
Candida albicans
-
inhibits hydrolysis of 4-methylumbelliferyl-alpha-D-mannopyranoside
-
0.0003 - 0.00047
kifunensine
0.47 - 0.62
swainsonine
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0000036
-
40C, pH 5.5
0.00003
-
purification of soluble alpha-mannosidase from CAI-4 strain, High-speed supernatant used to measure alpha-mannosidase activity
0.00008
-
purification of soluble alpha-mannosidase from CAI-4 strain, Sepharose CL6B
0.0002
-
mixed membrane fraction (MMF) of ATCC 26555 supernatant, expressed as nanomol of methylumbelliferone min-1mg-1 of protein
0.0005
-
mixed membrane fraction (MMF) of CAI-4 and ATCC 26555 plus 1 mM pepstatin supernatant, expressed as nanomol of methylumbelliferone min-1mg-1 of protein; mixed membrane fraction (MMF) of CAI-4 supernatant, expressed as nanomol of methylumbelliferone min-1mg-1 of protein
0.0012
-
mixed membrane fraction (MMF) of CAI-4 and ATCC 26555 pellet, expressed as nanomol of methylumbelliferone min-1mg-1 of protein
0.0019
-
mixed membrane fraction (MMF) of ATCC 26555 pellet, expressed as nanomol of methylumbelliferone min-1mg-1 of protein
0.0021
-
purification of soluble alpha-mannosidase from CAI-4 strain, DEAE Bio-Gel A
0.0022
-
mixed membrane fraction (MMF) of CAI-4 and ATCC 26555 plus 1 mM pepstatin pellet, expressed as nanomol of methylumbelliferone min-1mg-1 of protein
0.0026
-
mixed membrane fraction (MMF) of CAI-4 pellet, expressed as nanomol of methylumbelliferone min-1mg-1 of protein
0.003
-
purification of soluble alpha-mannosidase from CAI-4 strain, Sephadex G-25
0.0047
-
mixed membrane fraction (MMF) of CAI-4 and ATCC 26555 supernatant, expressed as nanomol of methylumbelliferone min-1mg-1 of protein
0.0057
-
purification of soluble alpha-mannosidase from CAI-4 strain, DEAE Bio-Gel A
0.0101
-
purification of soluble alpha-mannosidase from CAI-4 strain, Con A-Sepharose
0.0108
-
purification of soluble alpha-mannosidase from CAI-4 strain, Sephadex G-25
0.0908
Candida albicans kex-2-delta-mutant
0.0946
Candida albicans strain CAI4
34.6
-
37C, pH 5.0
4000
-
37C, pH 7.0
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.9 - 5.3
-
2-O-alpha-D-mannopyranosyl-D-mannose as substrate
5
-
Manalpha(1-2)Man as substrate
5.3
-
mutant enzyme E330Q
5.5
-
hydrolysis of 1,2-alpha-mannobiose
5.5 - 6.5
-
in the presence of egg yolk lyso-phosphatidylcholine
5.5 - 6
5.9 - 6.5
-
-
6
-
isozyme MANIb
6 - 6.2
-
-
6 - 6.5
6.5 - 7
isoform ManIA2
6.6
-
mutant enzyme E330Q/E599Q and D463N/E599Q
6.7
-
mutant enzyme E599Q
6.9
-
mutant enzyme H524A
7.1
-
wild-type enzyme
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4 - 5.8
-
about half-maximal activity at pH 4 and 5.8, 0.1 M acetate buffer
4.8 - 6.8
-
about half-maximal activity at pH 4.8 and 6.8
5.2 - 7.3
-
about half-maximal activity at pH 5.2 and 7.3, in the presence of egg yolk lyso-phosphatidylcholine
5.2 - 6.7
-
about half-maximal activity at pH 5.2 and 6.7
5.3 - 6.6
-
about half-maximal activity at pH 5.3 and 6.6
5.5 - 7
-
about half-maximal activity at pH 5.5 and 7
5.5 - 7.5
-
about half-maximal activity at pH 5.5 and 7.5, in the presence of soybean phosphatidylinositol
5.5 - 7
-
about half-maximal activity at pH 5.5 and 7; (Man)8(GlcNAc)2-peptide as substrate
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25
-
isozymes MANIa and MANIb
35
isoform ManIC
45
-
hydrolysis of 1,2-alpha-mannobiose
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
45 - 60
-
45C: optimum, 60C: 60% of maximal activity, 65C: 14% of maximal activity, hydrolysis of 1,2-alpha-mannobiose
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3.6
-
isoelectric focussing
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
In situ hybridization shows high levels of alpha1,2-mannosidase IB
Manually annotated by BRENDA team
levels of Golgi alpha1,2-mannosidase IC are similar in most tissues, with the exception of placenta and liver
Manually annotated by BRENDA team
-
Taka-diastase, enzyme-product from Sankyo Co.
Manually annotated by BRENDA team
-
commercially available under the name of Morushin
Manually annotated by BRENDA team
In situ hybridization shows high levels of alpha1,2-mannosidase IA; In situ hybridization shows high levels of alpha1,2-mannosidase IB
Manually annotated by BRENDA team
Golgi alpha1,2-mannosidase IA
Manually annotated by BRENDA team
Golgi alpha1,2-mannosidase I displays distinct developmental and tissue-specific expression. Expression of Golgi alpha 1,2-mannosidases (IA, IB, IC) investigated by Nothern blot analysis and in situ hybridization; Golgi alpha1,2-mannosidase I displays distinct developmental and tissue-specific expression. Expression of Golgi alpha 1,2-mannosidases (IA, IB, IC) investigated by Nothern blot analysis and in situ hybridization. In situ hybridization shows high levels of alpha1,2-mannosidase IB; Golgi alpha1,2-mannosidase I displays distinct developmental and tissue-specific expression. Expression of Golgi alpha 1,2-mannosidases (IA, IB, IC) investigated by Nothern blot analysis and in situ hydridization
Manually annotated by BRENDA team
-
commercially available under the name of Morushin
Manually annotated by BRENDA team
Golgi alpha1,2-mannosidase IA; Golgi alpha1,2-mannosidase IB
Manually annotated by BRENDA team
Golgi alpha1,2-mannosidase IA; In situ hybridization shows high levels of alpha1,2-mannosidase IB
Manually annotated by BRENDA team
Golgi alpha1,2-mannosidase IA
Manually annotated by BRENDA team
Golgi alpha1,2-mannosidase IB
Manually annotated by BRENDA team
Golgi alpha1,2-mannosidase IA; In situ hybridization shows high levels of alpha1,2-mannosidase IA; In situ hybridization shows high levels of alpha1,2-mannosidase IB, Golgi alpha1,2-mannosidase IB
Manually annotated by BRENDA team
Golgi alpha1,2-mannosidase IA; Golgi alpha1,2-mannosidase IB
Manually annotated by BRENDA team
additional information
detected in the coccidioidal T27K vaccine
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
with strain 22A1, the ratio of intracellular to extracellular 1,2-alpha-mannosidase activity is about 4:1
-
Manually annotated by BRENDA team
; presence of a 65000 Da membrane-bound isoform that is cleaved by protease Kex2 to soluble 52000 Da isoform E-I
-
Manually annotated by BRENDA team
-
with strain 22A1, the ratio of intracellular to extracellular 1,2-alpha-mannosidase activity is about 4:1
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
23000
-
analytical electrophoresis of the purified enzyme reveals two polypeptides of 52 and 23 kDa, 52000 Da polypeptide being responsible for enzyme activity as revealed by zymogram analysis
27000
-
analytical electrophoresis of the purified preparation reveals two polypeptides
39000
-
cytosolic enzyme, gel filtration
40000
-
gel filtration
49000
-
gel filtration
49200
-
sedimentation analysis
50000
-
gel filtration
56920
theoretical molecular weight. SDS-PAGE, Western blot analysis. A highly purified fraction is obtained after FPLC
57000
-
calculated from amino acid sequence