Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.113 extracted from

  • Kimura, Y.; Yamaguchi, O.; Suehisa, H.; Tagaki, S.
    In vitro hydrolysis of oligomannose-type sugar chains by an alpha-1,2-mannosidase from microsomes of developing castor bean cotyledons (1991), Biochim. Biophys. Acta, 1075, 6-11.
    View publication on PubMed
Search for more literature for 3.2.1.113

Inhibitors

Inhibitors Comment Organism Structure
1-deoxymannojirimycin
-
 Ricinus communis
Cd2+ strong Ricinus communis
Cu2+ strong Ricinus communis
EDTA
-
 Ricinus communis
Fe3+ strong Ricinus communis
Hg2+ strong Ricinus communis
additional information
-
 Ricinus communis
Sn2+ strong Ricinus communis
Ti4+ strong Ricinus communis
Tris
-
 Ricinus communis
Zn2+
-
 Ricinus communis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.26
-
 Man6GlcNAc 40°C, pH 5.5 Ricinus communis

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ slight activation Ricinus communis
Ca2+ activation Ricinus communis

Organism

Organism UniProt Comment Textmining
Ricinus communis
-
 castor bean
-

Purification (Commentary)

Purification (Comment) Organism
partial Ricinus communis

Source Tissue

Source Tissue Comment Organism Textmining
cotyledon
-
 Ricinus communis
-
seedling
-
 Ricinus communis
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0000036
-
 40°C, pH 5.5 Ricinus communis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Man6GlcNAc + H2O
-
 Ricinus communis Man5GlcNAc + D-mannose
-
 ?
Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAc + H2O pyridylamino derivative of Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-3)ManbetaGlcNAc2 Ricinus communis (Man)5(GlcNAc)2 + D-mannose i.e. pyridylamino derivative of (Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc ?
additional information no substrate: (Man)4-(GlcNAc)2 Ricinus communis ?
-
 ?
pyridylamino derivative of (Man)9(GlcNAc)2 + H2O
-
 Ricinus communis pyridylamino derivative of (Man)5(GlcNAc)2 + D-mannose after 120 h incubation, via specific intermediates: overview ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
 assay at Ricinus communis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5 6
-
 Ricinus communis