3.2.1.113: mannosyl-oligosaccharide 1,2-alpha-mannosidase

This is an abbreviated version!
For detailed information about mannosyl-oligosaccharide 1,2-alpha-mannosidase, go to the full flat file.

Reaction

Synonyms

(alpha1,2)-mannosidase-I, 1,2-alpha-mannosidase, alpha-(1->2)-mannosidase I, alpha-1,2 mannosidase I, alpha-1,2-mannosidase, alpha-1,2-mannosidase IC, alpha-1,2-mannosidaseI, alpha-1-2-mannosidase, alpha-mannosidase, alpha1,2-mannosidase, alpha1,2-mannosidase I, alpha1-2-mannosidase I, At1g51590, At3g21160, class I endoplasmic reticulum 1,2-alpha-mannosidase, D2030.1, endoplasmic reticulum mannosidase I, ER alpha-1,2-mannosidase, ER-Man, exo-alpha-1,2-mannanase, FmanIBp, fungal alpha-1,2-mannosidase, glycoprotein processing mannosidase I, Golgi alpha-mannosidase I, Golgi alpha1,2-mannosidase I, Golgi alpha1,2-mannosidase IA, Golgi alpha1,2-mannosidase IB, Golgi alpha1,2-mannosidase IC, Golgi alpha1,2-mannosidase II, GolgiManI, HMIC, Man(9)-alpha-mannosidase, MAN1A1, MAN1A2, MAN1B1, MAN1C1, Man9 processing alpha-mannosidase, Man9-alpha-mannosidase, Man9-mannosidase, Man9GlcNAc2-specific processing alpha-mannosidase, manE, ManI, MANIa, ManIA2, MANIb, ManIC, mannose-9 processing alpha-mannosidase, mannosidase 1A, mannosidase 1B, mannosidase I, mannosidase, exo-1,2-alpha-, Mas1, MIa, MIa,b,c, MIb, MIc, MII, MIIx, MNS1, Mns1p, MNS2, msdC, N-glycan processing class I alpha-1,2-mannosidase, processing alpha-1,2-mannosidase IC

ECTree

     3 Hydrolases

         3.2 Glycosylases

             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds

                3.2.1.113 mannosyl-oligosaccharide 1,2-alpha-mannosidase

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Results	in table
11	Activating Compound
209	AA Sequence
4	Application
1	CAS Registry Number
18	Cloned(Commentary)
7	Crystallization (Commentary)
34653	Disease/ Diagnostics
3	Expression
20	General Information
4	General Stability
11	IC50 Value
124	Inhibitors
4	Ki Value [mM]
13	KM Value [mM]
72	Localization
43	Metals/Ions
18	Molecular Weight [Da]
21	Natural Substrates/ Products (Substrates)
425	Organism
4	Pathway
30	PDB ID
23	pH Optimum
11	pH Range
4	pH Stability
3	pI Value
6	Posttranslational Modification
28	Protein Variants
27	Purification (Commentary)
10	Reaction
2	Reaction Type
76	Reference
65	Source Tissue
12	Specific Activity [micromol/min/mg]
9	Storage Stability
146	Substrates and Products (Substrate)
22	Subunits
111	Synonyms
1	Systematic Name
16	Temperature Optimum [°C]
1	Temperature Range [°C]
11	Temperature Stability [°C]
4	Turnover Number [1/s]

Inhibitors

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of the Enzyme Summary Page.

Inhibitors on EC 3.2.1.113 - mannosyl-oligosaccharide 1,2-alpha-mannosidase

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INHIBITOR

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

1,4-Dideoxy-1,4-imino-D-mannitol

4 entries

1-Cyclohexyl-3-(2-morpholinyl-4-ethyl)carbodiimide/glycine methylester

Aspergillus phoenicis

-

-

135991

1-deoxymannojirimycin

19 entries

Ag+

Aspergillus phoenicis

-

not

135991

alpha1,2-Linked oligosaccharides

Vigna radiata var. radiata

-

-

135992, 646582

alpha1,3-Linked oligosaccharides

Vigna radiata var. radiata

-

-

135992, 646582

Basic sugar analogs of mannose

Sus scrofa

-

strong

646583

-

Cd2+

Ricinus communis

-

strong

646587

Co2+

3 entries

Cu2+

5 entries

D-mannono-delta-lactam

Trypanosoma cruzi

-

-

646608

D-mannono-gamma-lactone

Trypanosoma cruzi

-

-

646608

D-mannonolactam amidrazone

2 entries

deoxymannojirimycin

2 entries

deoxymannojirimycin hydrochloride

Coccidioides posadasii

Q3HYC1

inhibitor at a final concentration of 0.5 mM, reduces the activity by 80%

677573

EDTA

13 entries

EGTA

2 entries

Fe2+

2 entries

Fe3+

Ricinus communis

-

strong

646587

Hg2+

Ricinus communis

-

strong

646587

iodoacetic acid

Aspergillus phoenicis

-

monoiodoacetic acid, 1 mM, weak

135991

kifunensine

11 entries

mannosamine

2 entries

methyl-alpha-D-lyxopyranosyl-(1',2)-alpha-D-mannopyranoside

Penicillium citrinum

P31723

analogue of the minimal disaccharide substrate mannobiose. Cocrystallization and mass spectrometry data suggest that when methyl-alpha-D-lyxopyranosyl-(1',2)-alpha-D-mannopyranoside is incubated with the mannosidase for long periods of time it is cleaved and that methyl-alpha-D-lyxopyranosyl-(1',2)-alpha-D-mannopyranoside behaves as a substrate

677330

methyl-alpha-mannoside

Aspergillus phoenicis

-

-

135991

Mn2+

Aspergillus phoenicis

-

-

135991

N-5-Carboxypentyldeoxymannojirimycin

2 entries

N-Methyl-deoxymannojirimycin

3 entries

Ni2+

Bos taurus

-

-

646579

p-chloromercuriphenylsulfonic acid

Rattus norvegicus

-

-

646585

PCMB

Aspergillus phoenicis

-

not

135991

Sn2+

Ricinus communis

-

strong

646587

swainsonine

4 entries

Ti4+

Ricinus communis

-

strong

646587

Tris

2 entries

Tris/maleate

Rattus norvegicus

-

-

646570

Zn2+

Ricinus communis

-

-

646587

additional information

23 entries

-

1,4-Dideoxy-1,4-imino-D-mannitol

Embryophyta

-

not

646588

1,4-Dideoxy-1,4-imino-D-mannitol

Rattus norvegicus

-

in vitro and in vivo

646576

1,4-Dideoxy-1,4-imino-D-mannitol

Rattus norvegicus

-

not

646588

1,4-Dideoxy-1,4-imino-D-mannitol

Vigna radiata var. radiata

-

not

135992, 646582

1-deoxymannojirimycin

Arabidopsis thaliana

-

isozyme MANIa shows 20% residual activity at 0.1 mM 1-deoxymannojirimycin, isozyme MANIb shows 45% residual activity at 0.1 mM 1-deoxymannojirimycin

703909

1-deoxymannojirimycin

Arabidopsis thaliana

Q8H116, Q9C512

-

751841

1-deoxymannojirimycin

Aspergillus oryzae

-

0.2 mM, 50% inhibition

664385

1-deoxymannojirimycin

Bacillus sp. (in: Bacteria)

-

not

646592

1-deoxymannojirimycin

Bos taurus

-

-

646579

1-deoxymannojirimycin

Candida albicans

-

-

753587

1-deoxymannojirimycin

Candida albicans

Q8J0Q0

-

754617

1-deoxymannojirimycin

Homo sapiens

-

-

135958

1-deoxymannojirimycin

Homo sapiens

-

mannose analogue, specific inhibitor of alpha1,2-mannosidase. Focus on the effect of blocking alpha-mannosidases I in human hepatocarcinoma 7721 cells concerning with endoplasmic reticulum stress. Observed that 1-deoxymannojirimycin can also be a radical stimulus in inducing endoplasmic reticulum stress

677921

1-deoxymannojirimycin

Homo sapiens

O60476, Q9NR34

no activity at 0.01 mM; no activity at 0.01 mM

715684

1-deoxymannojirimycin

Penicillium citrinum

P31723

modeling of binding to enzyme

754067

1-deoxymannojirimycin

Rattus norvegicus

-

reversible

646585

1-deoxymannojirimycin

Rattus norvegicus

-

mannose analog, in vivo and in vitro; rat liver enzyme

646588

1-deoxymannojirimycin

Ricinus communis

-

-

646587

1-deoxymannojirimycin

Spodoptera frugiperda

-

-

646591

1-deoxymannojirimycin

Sus scrofa

-

-

646583

1-deoxymannojirimycin

Sus scrofa

-

strong

646589

1-deoxymannojirimycin

Trypanosoma cruzi

-

not significantly

646608

1-deoxymannojirimycin

Vigna radiata var. radiata

-

-

135992, 646582

Co2+

Aspergillus phoenicis

-

-

135991

Co2+

Rattus norvegicus

-

weak

646570

Co2+

Rattus norvegicus

-

-

646585

Cu2+

Aspergillus phoenicis

-

-

135991

Cu2+

Bos taurus

-

-

646579

Cu2+

Rattus norvegicus

-

-

646570, 646585

Cu2+

Ricinus communis

-

strong

646587

Cu2+

Sus scrofa

-

-

646583

D-mannonolactam amidrazone

Oryctolagus cuniculus

-

inhibitor is a mimic of the mannopyranosyl cation and acts as a potent broad spectrum mannosidase inhibitor. In the presence of mannonoamidrazone, most of the oligosaccharides are not processed beyond the high mannose structure

754043

D-mannonolactam amidrazone

Vigna radiata var. radiata

-

inhibitor is a mimic of the mannopyranosyl cation and acts as a potent broad spectrum mannosidase inhibitor. It is inactive against processing glucosidases I and II

754043

deoxymannojirimycin

Homo sapiens

Q9UKM7

-

754053

deoxymannojirimycin

Mus musculus

-

-

706455

EDTA

Arabidopsis thaliana

-

isozyme MANIa shows 20% residual activity at 1 mM EDTA, isozyme MANIb shows 35% residual activity at 1 mM EDTA, isozyme MANIb activity in the presence of 1 mM EDTA is restored by the addition of Ca2+

703909

EDTA

Arabidopsis thaliana

Q8H116, Q9C512

-

751841

EDTA

Aspergillus oryzae

-

enzyme loses 96% activity after dialysis in 50 mM PIPES buffer, pH 6.0, containing 2 mM EDTA. Addition of 10 mM Ca2+ recovers activity to 100%, addition of 10 mM Mg2+ recovers activity to 43%

664385

EDTA

Aspergillus oryzae

-

complete inhibition at 10 mM

753758

EDTA

Aspergillus phoenicis

-

weak

135991

EDTA

Bacillus sp. (in: Bacteria)

-

-

646592

EDTA

Bos taurus

-

Ca2+ restores

646579

EDTA

Homo sapiens

Q9UKM7

the enzyme inhibition by EDTA can be reversed by the addition of Ca2+ and to a lesser extent by Fe2+ and Mn2+

754053

EDTA

Rattus norvegicus

-

weak

646570

EDTA

Rattus norvegicus

-

-

646585

EDTA

Ricinus communis

-

-

646587

EDTA

Trypanosoma cruzi

-

strong

646608

EDTA

Vigna radiata var. radiata

-

Ca2+ restores

135992, 646582

EGTA

Homo sapiens

Q9UKM7

the enzyme inhibition by EGTA can be reversed by the addition of Ca2+ and to a lesser extent by Fe2+ and Mn2+

754053

EGTA

Saccharomyces cerevisiae

-

complete inhibition at 5 mM

753754

Fe2+

Aspergillus phoenicis

-

-

135991

Fe2+

Trypanosoma cruzi

-

strong

646608

kifunensine

Arabidopsis thaliana

Q8H116, Q9C512

-

751841

kifunensine

Aspergillus oryzae

-

0.0015 mM, 50% inhibition

664385

kifunensine

Aspergillus oryzae

-

-

753758

kifunensine

Embryophyta

-

plant and animal Golgi mannosidase I

646588

kifunensine

Homo sapiens

O60476, Q9NR34

no activity at 0.01 mM; no activity at 0.01 mM

715684

kifunensine

Homo sapiens

Q9UKM7

-

754053

kifunensine

Homo sapiens

-

-

754069

kifunensine

Mus musculus

-

complete inhibition at 0.04 mM

706455

kifunensine

Penicillium citrinum

P31723

modeling of binding to enzyme

754067

kifunensine

Rattus norvegicus

-

plant and animal Golgi mannosidase I

646588

kifunensine

Trypanosoma cruzi

-

-

646608

mannosamine

Aspergillus phoenicis

-

not

135991

mannosamine

Vigna radiata var. radiata

-

-

135992, 646582

N-5-Carboxypentyldeoxymannojirimycin

Bos taurus

-

-

646579

N-5-Carboxypentyldeoxymannojirimycin

Sus scrofa

-

strong

646589

N-Methyl-deoxymannojirimycin

Bos taurus

-

-

646579

N-Methyl-deoxymannojirimycin

Rattus norvegicus

-

-

646588

N-Methyl-deoxymannojirimycin

Sus scrofa

-

-

646583

swainsonine

Candida albicans

-

-

753587

swainsonine

Candida albicans

Q8J0Q0

-

754617

swainsonine

Homo sapiens

O60476, Q9NR34

isoform ManIC shows 86% activity at 0.5 mM swainsonine

715684

swainsonine

Trypanosoma cruzi

-

-

646608

Tris

Rattus norvegicus

-

-

646585

Tris

Ricinus communis

-

-

646587

additional information

Arabidopsis thaliana

-

not inhibited by swainsonine

703909

-

additional information

Arabidopsis thaliana

Q8H116

not inhibitory: swainsonine; not inhibitory: swainsonine

751841

-

additional information

Arabidopsis thaliana

Q9C512

not inhibitory: swainsonine; not inhibitory: swainsonine

751841

-

additional information

Aspergillus phoenicis

-

no inhibition by 4-nitrophenyl-alpha-mannoside, mannitol, L-mannono-1,4-lactone, PMSF

135991

-

additional information

Embryophyta

-

-

646588

-

additional information

Homo sapiens

-

no inhibition by swainsonine

135958

-

additional information

Homo sapiens

O60476

isoform ManIA2 is not influenced by swainsonine

715684

-

additional information

Homo sapiens

Q9NR34

isoform ManIA2 is not influenced by swainsonine

715684

-

additional information

Homo sapiens

Q9UKM7

not inhibited by swainsonine

754053

-

additional information

Homo sapiens

-

not inhibited by swainsonine

754053

-

additional information

Nicotiana tabacum

-

cycloheximide, protein synthesis inhibitor. After a 2 h treatment with the protein synthesis inhibitor cycloheximide, ER and Golgi labeling observed with ManI-GFP fusion remains unchanged, showing that the steady state localization of ManI-GFP is the Golgi and the ER

682320

-

additional information

Penicillium citrinum

-

comparison with 1-deoxymannojirimycin and kifunensine

677330

-

additional information

Penicillium citrinum

P31723

comparison with 1-deoxymannojirimycin and kifunensine

677330

-

additional information

Rattus norvegicus

-

mannosidase IA and B are quite similar in response to inhibitors

135998

-

additional information

Rattus norvegicus

-

no inhibition by Ca2+; no inhibition by Mg2+

646585

-

additional information

Rattus norvegicus

-

no inhibition by mannostatin A

646588

-

additional information

Ricinus communis

-

-

646587

-

additional information

Spodoptera frugiperda

-

-

646591

-

additional information

Sus scrofa

-

no inhibition by swainsonine; no or little inhibition by N,N-dimethyl-1-deoxymannojirimycin

646583

-

additional information

Sus scrofa

-

-

646589

-

additional information

Vigna radiata var. radiata

-

no inhibition by mannose, alpha1,6-linked mannose oligosaccharide, castanospermine, deoxynojirimycin, 2,5-dihydroxymethyl-3,4-dihydroxypyrrolidine

135992

-

additional information

Vigna radiata var. radiata

-

no inhibition by mannose, alpha1,6-linked mannose oligosaccharide, castanospermine, deoxynojirimycin, 2,5-dihydroxymethyl-3,4-dihydroxypyrrolidine

646582

-

additional information

Vigna radiata var. radiata

-

not inhibitory up to 0.25 mM: mannostatin A

754043

-