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3.2.1.113: mannosyl-oligosaccharide 1,2-alpha-mannosidase

This is an abbreviated version!
For detailed information about mannosyl-oligosaccharide 1,2-alpha-mannosidase, go to the full flat file.

Word Map on EC 3.2.1.113

Reaction

Man6GlcNAc2-[protein] (isomer 6A1,2,3)
+
H2O
=
Man5GlcNAc2-[protein]
 +
beta-D-mannopyranose

Synonyms

(alpha1,2)-mannosidase-I, 1,2-alpha-mannosidase, alpha-(1->2)-mannosidase I, alpha-1,2 mannosidase I, alpha-1,2-mannosidase, alpha-1,2-mannosidase IC, alpha-1,2-mannosidaseI, alpha-1-2-mannosidase, alpha-mannosidase, alpha1,2-mannosidase, alpha1,2-mannosidase I, alpha1-2-mannosidase I, At1g51590, At3g21160, class I endoplasmic reticulum 1,2-alpha-mannosidase, D2030.1, endoplasmic reticulum mannosidase I, ER alpha-1,2-mannosidase, ER-Man, exo-alpha-1,2-mannanase, FmanIBp, fungal alpha-1,2-mannosidase, glycoprotein processing mannosidase I, Golgi alpha-mannosidase I, Golgi alpha1,2-mannosidase I, Golgi alpha1,2-mannosidase IA, Golgi alpha1,2-mannosidase IB, Golgi alpha1,2-mannosidase IC, Golgi alpha1,2-mannosidase II, GolgiManI, HMIC, Man(9)-alpha-mannosidase, MAN1A1, MAN1A2, MAN1B1, MAN1C1, Man9 processing alpha-mannosidase, Man9-alpha-mannosidase, Man9-mannosidase, Man9GlcNAc2-specific processing alpha-mannosidase, manE, ManI, MANIa, ManIA2, MANIb, ManIC, mannose-9 processing alpha-mannosidase, mannosidase 1A, mannosidase 1B, mannosidase I, mannosidase, exo-1,2-alpha-, Mas1, MIa, MIa,b,c, MIb, MIc, MII, MIIx, MNS1, Mns1p, MNS2, msdC, N-glycan processing class I alpha-1,2-mannosidase, processing alpha-1,2-mannosidase IC

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.113 mannosyl-oligosaccharide 1,2-alpha-mannosidase

Cloned

Cloned on EC 3.2.1.113 - mannosyl-oligosaccharide 1,2-alpha-mannosidase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
DNA of the Penicillium citrinum alpha-1,2 mannosidase gene lacking the signal sequence is cloned downstream of the Aspergillus amylase promoter and of the aspergillopepsin signal sequence. The resulting fungal expression vector pTAPM1 is transfected into Aspergillus oryzae strain MS2
enzyme-protein A-fusion protein is expressed in COS cells
expressed in Aspergillus oryzae strain MS2
expressed in Escherichia coli BL21 Star (DE3) cells
expressed in Escherichia coli JM109 (DE3) cells
expressed in HEK-293 cells
-
expressed in Nicotiana benthamiana leaf epidermal cells
expressed in Pichia pastoris
expressed in Sf9 cells as insoluble forms and in Escherichia coli as soluble forms
-
expressed in TOP10 chemically competent Escherichia coli cells
expression by an Aspergillus host
-
expression in Escherichia coli
-
expression in Pichia pastoris
GFP-fusions are transferred into Agrobacterium tumefaciens by heat shock
MAN1C1 gene over-expression is used to transfect human renal cancer cell lines 786-O and OS-RC-2