Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

3.2.1.113: mannosyl-oligosaccharide 1,2-alpha-mannosidase

This is an abbreviated version!
For detailed information about mannosyl-oligosaccharide 1,2-alpha-mannosidase, go to the full flat file.

Word Map on EC 3.2.1.113

Reaction

Man6GlcNAc2-[protein] (isomer 6A1,2,3)
+
H2O
=
Man5GlcNAc2-[protein]
 +
beta-D-mannopyranose

Synonyms

(alpha1,2)-mannosidase-I, 1,2-alpha-mannosidase, alpha-(1->2)-mannosidase I, alpha-1,2 mannosidase I, alpha-1,2-mannosidase, alpha-1,2-mannosidase IC, alpha-1,2-mannosidaseI, alpha-1-2-mannosidase, alpha-mannosidase, alpha1,2-mannosidase, alpha1,2-mannosidase I, alpha1-2-mannosidase I, At1g51590, At3g21160, class I endoplasmic reticulum 1,2-alpha-mannosidase, D2030.1, endoplasmic reticulum mannosidase I, ER alpha-1,2-mannosidase, ER-Man, exo-alpha-1,2-mannanase, FmanIBp, fungal alpha-1,2-mannosidase, glycoprotein processing mannosidase I, Golgi alpha-mannosidase I, Golgi alpha1,2-mannosidase I, Golgi alpha1,2-mannosidase IA, Golgi alpha1,2-mannosidase IB, Golgi alpha1,2-mannosidase IC, Golgi alpha1,2-mannosidase II, GolgiManI, HMIC, Man(9)-alpha-mannosidase, MAN1A1, MAN1A2, MAN1B1, MAN1C1, Man9 processing alpha-mannosidase, Man9-alpha-mannosidase, Man9-mannosidase, Man9GlcNAc2-specific processing alpha-mannosidase, manE, ManI, MANIa, ManIA2, MANIb, ManIC, mannose-9 processing alpha-mannosidase, mannosidase 1A, mannosidase 1B, mannosidase I, mannosidase, exo-1,2-alpha-, Mas1, MIa, MIa,b,c, MIb, MIc, MII, MIIx, MNS1, Mns1p, MNS2, msdC, N-glycan processing class I alpha-1,2-mannosidase, processing alpha-1,2-mannosidase IC

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.113 mannosyl-oligosaccharide 1,2-alpha-mannosidase

Temperature Stability

Temperature Stability on EC 3.2.1.113 - mannosyl-oligosaccharide 1,2-alpha-mannosidase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
20 - 30
isoform ManIA2 shows more than 75% activity at 30°C in the presence of Ca2+, and more than 75% activity at 20°C in the absence of Ca2+
25
-
isozymes MANIa and MANIb are relatively stable at lower temperature and still retain 50% of their original activities after incubation at 25°C for 1 h and show a significant decrease of activities at higher temperatures with 10% activity at 30°C
37
-
24 h, in 0.1 M sodium acetate buffer, pH 5.8, 30% loss of activity
40
isoform ManIC shows more than 75% activity at 40°C in the absence and presence of Ca2+
52
-
Tm-value of mutant enzyme C443A
53
-
Tm-value of mutant enzyme C443T
56
-
Tm-value of wild-type enzyme
additional information
-
mannosidase IA and B differ in thermostability