Any feedback?
Literature summary for 3.2.1.113 extracted from
- Glycoprotein processing enzymes of plants (1989), Methods Enzymol., 179, 452-475.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Triton X-100 | activation | Vigna radiata var. radiata |  |
| Triton X-100 | 0.1% | Vigna radiata var. radiata | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,4-Dideoxy-1,4-imino-D-mannitol | not | Vigna radiata var. radiata | |
| 1-deoxymannojirimycin | - |
Vigna radiata var. radiata | |
| alpha1,2-Linked oligosaccharides | - |
Vigna radiata var. radiata | |
| alpha1,3-Linked oligosaccharides | - |
Vigna radiata var. radiata | |
| EDTA | Ca2+ restores | Vigna radiata var. radiata | |
| mannosamine | - |
Vigna radiata var. radiata | |
| additional information | no inhibition by mannose, alpha1,6-linked mannose oligosaccharide, castanospermine, deoxynojirimycin, 2,5-dihydroxymethyl-3,4-dihydroxypyrrolidine | Vigna radiata var. radiata |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Vigna radiata var. radiata | 16020 | - |
| microsome | - |
Vigna radiata var. radiata | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | activation | Vigna radiata var. radiata | |
| Ca2+ | 5 mM | Vigna radiata var. radiata | |
| Mg2+ | activation | Vigna radiata var. radiata | |
| Mg2+ | slight | Vigna radiata var. radiata | |
| Mn2+ | - |
Vigna radiata var. radiata | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + 4 H2O | Vigna radiata var. radiata | - |
(Man)5GlcNAc + 4 D-mannose | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Vigna radiata var. radiata | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Vigna radiata var. radiata |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Vigna radiata var. radiata |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, in buffer with 10% glycerol, a few days, at early stage of purification at least 2 weeks | Vigna radiata var. radiata |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + 4 H2O | - |
Vigna radiata var. radiata | (Man)5GlcNAc + 4 D-mannose | - |
? | |
| Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + 4 H2O | removes all 4 alpha1,2-linked mannosyl residues | Vigna radiata var. radiata | (Man)5GlcNAc + 4 D-mannose | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Vigna radiata var. radiata |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 6 | - |
Vigna radiata var. radiata |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
