3.1.6.14: N-acetylglucosamine-6-sulfatase

This is an abbreviated version, for detailed information about N-acetylglucosamine-6-sulfatase, go to the full flat file.

Reaction

hydrolysis of the 6-sulfate groups of the N-acetyl-D-glucosamine 6-sulfate units of heparan sulfate and keratan sulfate =

Synonyms

2-acetamido-2-deoxy-D-glucose 6-sulfate sulfatase, 6-O-sulfatase, acetylglucosamine 6-sulfatase, chondroitinsulfatase, G6S, glucosamine 6-O-sulfatase, glucosamine-6-sulfatase, glucosamine-6-sulfate sulfatase, heparin/heparan sulfate 6-O-sulfatase, HSGAG sulfatase, N-acetylglucosamine 6-sulfate sulfatase, N-acetylglucosamine-6-sulfatase, NG6S, O,N-disulfid O-sulfohydrolase, sulfatase, acetylglucosamine 6-

ECTree

     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.6 Sulfuric-ester hydrolases
                3.1.6.14 N-acetylglucosamine-6-sulfatase

Substrates Products

Substrates Products on EC 3.1.6.14 - N-acetylglucosamine-6-sulfatase

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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
4-methylumbelliferyl N-acetyl-beta-D-glucosamine 6-sulfate + H2O
4-methylumbelliferyl N-acetyl-beta-D-glucosamine + sulfate
show the reaction diagram
-
-
-
-
?
4-methylumbelliferyl sulfate + H2O
4-methylumbelliferone + sulfate
show the reaction diagram
-
fluorogenic substrate
-
-
?
alpha-D-glucosamine N2,O6-bissulfate + H2O
?
show the reaction diagram
-
-
-
-
?
beta-D-GlcNAc6S-(1->4)-beta-D-GlcA-(1->4)-2,5-anhydro-D-mannitol + H2O
beta-D-GlcNAc-(1->4)-beta-D-GlcA-(1->4)-2,5-anhydro-D-mannitol + sulfate
show the reaction diagram
-
-
-
-
?
glucose 6-sulfate + H2O
glucose + sulfate
show the reaction diagram
N-acetyl galactosamine 6-sulfate + H2O
N-acetyl galactosamine + sulfate
show the reaction diagram
-
very low activity in cultured skin fibroblasts
-
-
?
N-acetyl-D-glucosamine 6-sulfate + H2O
N-acetyl-D-glucosamine + sulfate
show the reaction diagram
N-acetyl-D-glucosamine 6-sulfate units + H2O
N-acetyl-D-glucosamine units + sulfate
show the reaction diagram
O-(alpha-2-sulfamino glucosamine 6-sulfate)-(1-4)-L-idonic acid + H2O
O-(alpha-2-sulfamino glucosamine)-(1-4)-L-idose + sulfate
show the reaction diagram
O-(alpha-glucosamine 6-sulfate)-(1-3)-L-idonic acid + H2O
O-(alpha-glucosamine)-(1-3)-L-idonic acid + sulfate
show the reaction diagram
O-(alpha-glucosamine 6-sulfate)-(1-4)-L-O-(alpha-iduronic acid 2-sulfate)-(1-4)-D-O-2,5-anhydro mannitol 6-sulfate + H2O
O-(alpha-glucosamine)-(1-4)-L-O-(alpha-iduronic acid 2-sulfate)-(1-4)-D-O-2,5-anhydro mannitol 6-sulfate + sulfate
show the reaction diagram
O-(alpha-N-acetyl glucosamine 6-sulfate)-(1-3)-L-idonic acid + H2O
O-(alpha-N-acetyl glucosamine)-(1-3)-L-idonic acid + sulfate
show the reaction diagram
O-(alpha-N-acetyl glucosamine 6-sulfate)-(1-4)-L-idose-2-sulfate + H2O
O-(alpha-N-acetyl glucosamine)-(1-4)-L-idose-2-sulfate + sulfate
show the reaction diagram
-
same activity in cultured skin fibroblasts compared to monosaccharides
-
-
?
O-(alpha-N-acetyl glucosamine 6-sulfate)-(1-4)-L-iduronic acid-2-sulfate-(1-4)-2,5-anhydro-D-mannitol-6-sulfate + H2O
O-(alpha-N-acetyl glucosamine)-(1-4)-L-iduronic acid-2-sulfate-(1-4)-2,5-anhydro-D-mannitol-6-sulfate + sulfate
show the reaction diagram
-
30 fold higher activity in cultured skin fibroblasts compared to di- and monosaccharides
-
-
?
O-(beta-N-acetyl glucosamine 6-sulfate)-(1-3)-D-galactitol + H2O
O-(alpha-N-acetyl glucosamine)-(1-3)-D-galactitol + sulfate
show the reaction diagram
-
activity 100-fold less
-
-
?
trisaccharide derived from heparansulfate with a N-acetylglucosamine 6-sulfate residue + H2O
trisaccharide with a N-acetylglucosamine + sulfate
show the reaction diagram
additional information
?
-