Cloned (Comment) | Organism |
---|---|
gene GNS, quantitative real-time PCR enzyme expression analysis | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | silencing the expression of Sulf-1 by RNA interference restores the responses induced by FGF-1, which indicates that TNF-alpha-mediated induction of the sulfatase results in alterations of HS biological properties | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cell membrane | the heterodimeric enzyme is released in extracellular environment and/or retained at the cell membrane, probably by interaction with cell surface heparan sulfate | Homo sapiens | - |
- |
extracellular | the heterodimeric enzyme is released in extracellular environment and/or retained at the cell membrane, probably by interaction with cell surface heparan sulfate | Homo sapiens | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P15586 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | Sulf-1 is synthesized as a about 125-kDa precursor that is cleaved by a furin-type proteinase. This process results in the formation of an active heterodimer, in which 75-kDa and 50-kDa subunits are linked by disulfide bonds | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fibroblast | - |
Homo sapiens | - |
MRC5-SV2 cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methylumbelliferyl sulfate + H2O | fluorogenic substrate 4-MUS | Homo sapiens | 4-methylumbelliferone + sulfate | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | Sulf-1 is synthesized as a about 125-kDa precursor that is cleaved by a furin-type proteinase. This process results in the formation of an active heterodimer, in which 75-kDa and 50-kDa subunits are linked by disulfide bonds. The enzyme is then released in extracellular environment and/or retained at the cell membrane, probably by interaction with cell surface heparan sulfate | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
heparan sulfate 6-O-endosulfatase | - |
Homo sapiens |
heparan sulfate 6-O-endosulfatase1 | - |
Homo sapiens |
HS 6-O-endosulfatase | - |
Homo sapiens |
Sulf-1 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Homo sapiens |
Organism | Comment | Expression |
---|---|---|
Homo sapiens | tumour-necrosis factor-alpha (TNF-alpha) induces heparan sulfate 6-O-endosulfatase1 (Sulf-1) expression in fibroblasts via a mechanism involving NF-kappaB, ERK1/2, and p38 MAPK. Cell stimulation with TNF-alpha is accompanied by the secretion of an active form of Sulf-1. Regulation of Sulf-1 expression by TNF-alpha, overview | up |
General Information | Comment | Organism |
---|---|---|
additional information | TNF-alpha-treated cells show a strong reduction in the amount of the trisulfated UA2S-GlcNS6S disaccharide, suggesting a selective reaction of 6-O-desulfation. Cell stimulation with TNF-alpha is accompanied by the secretion of an active form of Sulf-1 | Homo sapiens |