Literature summary for 3.1.6.14 extracted from

Myette, J.R.; Soundararajan, V.; Shriver, Z.; Raman, R.; Sasisekharan, R.
Heparin/heparan sulfate 6-O-sulfatase from Flavobacterium heparinum: integrated structural and biochemical investigation of enzyme active site and substrate specificity (2009), J. Biol. Chem., 284, 35177-35188.

Search for more literature for 3.1.6.14

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Pedobacter heparinus

Protein Variants

Protein Variants	Comment	Organism
C80A	inactive	Pedobacter heparinus
D186A	53% relative activity after 0.5 h and 92% relative activity after 5 h compared to the wild type enzyme	Pedobacter heparinus
D374A	inactive	Pedobacter heparinus

General Stability

General Stability	Organism
the enzyme shows higher activity in acetate buffer when compared with sulfonate buffers such as MES and MOPS	Pedobacter heparinus

Inhibitors

Inhibitors	Comment	Organism	Structure
EDTA	less than 20% activity in the presence of 1 mM EDTA	Pedobacter heparinus
phosphate	-	Pedobacter heparinus
sulfate	-	Pedobacter heparinus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	stimulated 2-3fold by 5 mM Ca2+	Pedobacter heparinus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
61570	-	calculated from amino acid sequence	Pedobacter heparinus

Organism

Organism	UniProt	Comment	Textmining
Pedobacter heparinus	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-methylumbelliferyl N-acetyl-beta-D-glucosamine 6-sulfate + H2O	-	Pedobacter heparinus	4-methylumbelliferyl N-acetyl-beta-D-glucosamine + sulfate	-	?
alpha-D-glucosamine N2,O6-bissulfate + H2O	-	Pedobacter heparinus	?	-	?
additional information	heparin/heparan sulfate 6-O-sulfatase is a predominantly exolytic enzyme that specifically acts on N-sulfated or N-acetylated 6-O-sulfated glucosamines present at the non-reducing end of heparin and heparan sulfate glycosaminoglycan oligosaccharide substrates	Pedobacter heparinus	?	-	?
N-acetyl-D-glucosamine 6-sulfate + H2O	-	Pedobacter heparinus	N-acetyl-D-glucosamine + sulfate	-	?

Synonyms

Synonyms	Comment	Organism
6-O-sulfatase	-	Pedobacter heparinus
glucosamine 6-O-sulfatase	-	Pedobacter heparinus
heparin/heparan sulfate 6-O-sulfatase	-	Pedobacter heparinus
HSGAG sulfatase	-	Pedobacter heparinus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
5.5	6.5	-	Pedobacter heparinus

pI Value

Organism	Comment	pI Value Maximum	pI Value
Pedobacter heparinus	calculated from amino acid sequence	-	8.6

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
2	-	-	Pedobacter heparinus	phosphate
20	-	-	Pedobacter heparinus	sulfate

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Release 2023.1 (January 2023)