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Literature summary for 3.1.6.14 extracted from

  • Freeman, C.; Hopwood, J.J.
    Sanfilippo D syndrome: estimation of N-acetylglucosamine-6-sulfatase activity with a radiolabeled monosulfated disaccharide substrate (1989), Anal. Biochem., 176, 244-248.
    View publication on PubMed
Search for more literature for 3.1.6.14

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol stimulates only the purified enzyme Homo sapiens

Application

Application Comment Organism
medicine Sanfilippo D Syndrome: N-acetylglucosamine 6-sulfate sulfatase deficiency, mucopolysaccharidosis type IIID Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
bovine serum albumin inhibits only the purified enzyme Homo sapiens
NaCl
-
 Homo sapiens
PO43-
-
 Homo sapiens
SO42-
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0072
-
 O-(alpha-N-acetyl glucosamine 6-sulfate)-(1-3)-L-idonic acid in fibroblasts Homo sapiens
0.0153
-
 O-(alpha-N-acetyl glucosamine 6-sulfate)-(1-3)-L-idonic acid in leucocytes Homo sapiens
0.027
-
 O-(alpha-N-acetyl glucosamine 6-sulfate)-(1-3)-L-idonic acid in urine Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N-acetyl-D-glucosamine 6-sulfate units + H2O Homo sapiens in oligo-and monosaccharides derived from heparan sulfate and keratan sulfate N-acetyl-D-glucosamine units + sulfate
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
amnion amniotic cell culture Homo sapiens
-
chorionic villus in culture Homo sapiens
-
leukocyte
-
 Homo sapiens
-
skin fibroblast in culture Homo sapiens
-
urine
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-acetyl-D-glucosamine 6-sulfate units + H2O
-
 Homo sapiens N-acetyl-D-glucosamine units + sulfate
-
 ?
N-acetyl-D-glucosamine 6-sulfate units + H2O in oligo-and monosaccharides derived from heparan sulfate and keratan sulfate Homo sapiens N-acetyl-D-glucosamine units + sulfate
-
 ?
O-(alpha-N-acetyl glucosamine 6-sulfate)-(1-3)-L-idonic acid + H2O
-
 Homo sapiens O-(alpha-N-acetyl glucosamine)-(1-3)-L-idonic acid + sulfate
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2
-
 in fibroblasts Homo sapiens
4.5
-
 in leukocytes Homo sapiens
5.1
-
 in urine Homo sapiens