3.1.25.1: deoxyribonuclease (pyrimidine dimer)
This is an abbreviated version!
For detailed information about deoxyribonuclease (pyrimidine dimer), go to the full flat file.
Word Map on EC 3.1.25.1
-
3.1.25.1
-
glycosylase
-
strand
-
comet
-
thymine
-
genotoxic
-
formamidopyrimidine
-
cyclobutane
-
formamidopyrimidine-dna
-
incision
-
abasic
-
uv-induced
-
endonucleases
-
uv-irradiated
-
photoproducts
-
duplex
-
xeroderma
-
pigmentosum
-
dimer-specific
-
endoiii
-
8-oxoguanine
-
beta-elimination
-
8-oxog
-
repair-deficient
-
alkali-labile
-
apyrimidinic
-
ultraviolet-irradiated
-
photolyase
-
lesion-specific
-
apurinic
-
uv-damaged
-
helix-hairpin-helix
-
iii-sensitive
-
dna-glycosylase
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7,8-dihydro-8-oxoguanine
-
fpg-sensitive
-
8-hydroxyguanine
-
3-methyladenine-dna
-
uracil-dna
-
5-hydroxycytosine
-
incises
-
cis-syn
-
dipyrimidine
-
medicine
-
molecular biology
-
repair-proficient
-
dimer-containing
-
enzyme-dna
-
8-oxo-7,8-dihydroguanine
-
analysis
-
mitogenome
-
excision-repair
-
photoreactivation
-
5-hydroxyuracil
- 3.1.25.1
- glycosylase
- strand
- comet
- thymine
-
genotoxic
- formamidopyrimidine
-
cyclobutane
-
formamidopyrimidine-dna
-
incision
-
abasic
-
uv-induced
- endonucleases
-
uv-irradiated
- photoproducts
- duplex
- xeroderma
- pigmentosum
-
dimer-specific
-
endoiii
- 8-oxoguanine
-
beta-elimination
-
8-oxog
-
repair-deficient
-
alkali-labile
-
apyrimidinic
-
ultraviolet-irradiated
-
photolyase
-
lesion-specific
-
apurinic
-
uv-damaged
-
helix-hairpin-helix
-
iii-sensitive
-
dna-glycosylase
- 7,8-dihydro-8-oxoguanine
-
fpg-sensitive
- 8-hydroxyguanine
-
3-methyladenine-dna
-
uracil-dna
- 5-hydroxycytosine
-
incises
-
cis-syn
-
dipyrimidine
- medicine
- molecular biology
-
repair-proficient
-
dimer-containing
-
enzyme-dna
- 8-oxo-7,8-dihydroguanine
- analysis
-
mitogenome
-
excision-repair
-
photoreactivation
- 5-hydroxyuracil
Reaction
endonucleolytic cleavage near pyrimidine dimers to products with 5'-phosphate =
Synonyms
ApeI, bacteriophage T4 endodeoxyribonuclease V, bacteriophage T4 endonuclease V, correndonuclease I, correndonuclease II, endodeoxyribonuclease (pyrimidine dimer), endonuclease II, endonuclease III, endonuclease V, Escherichia coli UV endonuclease, excision endonuclease UvrABC, excision nuclease UvrABC, More, nuclease V, bacteriophage T4 endodeoxyribonuclease V, nuclease, Escherichia coli UV-endodeoxyribonuclease, Py-Py-correndonuclease, ribosomal protein S3, rpS3, T4 Endo V, T4 endonuclease V, T4N5, type I AP endonuclease, UvrA, UvrABC nuclease, UvrB, UvrC
ECTree
3.1.25.1 deoxyribonuclease (pyrimidine dimer)Advanced search results
results (
results (Substrates Products
Substrates Products on EC 3.1.25.1 - deoxyribonuclease (pyrimidine dimer)
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
DNA with gamma-radiation-induced cross-linked guanine-thymine tandem lesion G[8,5-Me]T + H2O
?
-
the DNA substrate contains a covalent bond between C8 of the guanine and the 5-methyl carbon of the 3'-thymine, formed by X-radiation
-
-
?
DNA with gamma-radiation-induced cross-linked thymine-thymine lesion T[6,4]T + H2O
?
-
6-4 pyrimidine-pyrimidone photoproduct
-
-
?
dsDNA + H2O
?
Tequatrovirus T4
-
excision of cyclobutane pyrimidine dimers TT and TU, the latter being a cis-syn cyclobutane thymine-uracil dimer, produced within duplex DNA by UV radiation with equal efficiency, U is derived from C which becomes deaminated by UV radiation
-
-
?
glycosylated DNA + H2O
oligonucleotides
Tequatrovirus T4
-
UV-irradiated, cleaves also nonglycosylated DNA
-
?
Haemophilus influenzae DNA + H2O
oligonucleotides
-
UV irradiated
-
?
M-13 DNA + H2O
oligonucleotides
Tequatrovirus T4
-
UV-irradiated, single-stranded DNA
-
?
poly(dI)-poly(dC) + H2O
oligonucleotides
-
UV irradiated, in 50 mM phosphate or 10 mM Tris-HCl supplemented with 100 mM NaCl
-
?
superhelical DNA + H2O
oligonucleotides
Tequatrovirus T4
-
UV-irradiated, cleaves also linear DNA
-
?
damaged DNA containing nucleotide cross-links + H2O
?
-
initiation of nucleotide excision repair of DNA-polypeptide cross-links by the UvrABC nuclease
-
-
?
damaged DNA containing nucleotide cross-links + H2O
?
-
initiation of nucleotide excision repair of DNA-polypeptide cross-links by the UvrABC nuclease, engineering of DNA-peptide or DNA-protein cross-links via linkage at an acrolein-derived gamma-hydroxypropanodeoxyguanosine adduct and an apurinic/apyrimidinic site, the linked peptides are KWKK and KFHEKHHSHRGY, and the protein is the 16 kDa T4 pyrimidine dimer glycosylase/apurinic/apyrimidinic site lyase, incision at the eighth phosphodiester bond 5' of the lesion
-
-
?
damaged DNA containing nucleotide cross-links + H2O
?
-
initiation of nucleotide excision repair of DNA-polypeptide cross-links by the UvrABC nuclease
-
-
?
damaged DNA containing nucleotide cross-links + H2O
?
-
initiation of nucleotide excision repair of DNA-polypeptide cross-links by the UvrABC nuclease, engineering of DNA-peptide or DNA-protein cross-links via linkage at an acrolein-derived gamma-hydroxypropanodeoxyguanosine adduct and an apurinic/apyrimidinic site, the linked peptides are KWKK and KFHEKHHSHRGY, and the protein is the 16 kDa T4 pyrimidine dimer glycosylase/apurinic/apyrimidinic site lyase, incision at the eighth phosphodiester bond 5' of the lesion
-
-
?
DNA + H2O
?
Tequatrovirus T4
-
-
170694, 170695, 170696, 170697, 170698, 170699, 170700, 170701, 170702, 170703, 170704, 170705, 170706, 170712, 170713, 170714, 170715
-
-
?
DNA + H2O
?
Tequatrovirus T4
-
application of the enzyme to humans suffering the disease xeroderma pigmentosum due to DNA damage by UV radiation reverses the defective repair in these cells, the enzyme influences DNA repair, mutagenesis, and cell signaling, enhanced DNA repair of cyclobutane pyrimidine dimers changes the biological response to UV-B radiation, overview
-
-
?
DNA + H2O
?
Tequatrovirus T4
-
first reaction step of the repair pathway to excise pyrimidine-dimers
-
-
?
DNA + H2O
?
Tequatrovirus T4
-
excision of pyrimidine dimers produced within duplex DNA by UV radiation
-
-
?
DNA + H2O
?
Tequatrovirus T4
excision of pyrimidine dimers produced within duplex DNA by UV radiation, specific for thymine-dimers, interaction with wild-type and duplex DNA, overview
-
-
?
DNA + H2O
?
Tequatrovirus T4
-
excision of thymine dimers produced within duplex DNA by UV radiation
-
-
?
DNA containing apurinic/apyrimidinic sites + H2O
?
-
the enzyme is endonucleolytically active on AP sites generated from pyrimidine dimers cleaving phosphodiester bonds of the damaged DNAs with broad specificity
-
-
?
DNA containing apurinic/apyrimidinic sites + H2O
?
-
the enzyme is endonucleolytically active on AP sites generated from pyrimidine dimers cleaving phosphodiester bonds of the damaged DNAs with broad specificity
-
-
?
oligodeoxyribonucleotides
oligonucleotides
-
dimer containing 49mer
-
?
oligodeoxyribonucleotides
oligonucleotides
Tequatrovirus T4
-
UV-irradiated, 14mer and 18mer, single-stranded oligomers are a poorer substrate than the corresponding duplexes
-
?
oligodeoxyribonucleotides
oligonucleotides
Tequatrovirus T4
-
14mer containing a TT-dimer, duplex
-
?
oligonucleotide DNA + H2O
?
-
-
-
-
?
oligonucleotide DNA + H2O
?
-
-
-
-
?
PBS1 DNA
oligonucleotides
Tequatrovirus T4
-
UV irradiated, contains uracil instead of thymine
-
?
PBS1 DNA
oligonucleotides
Tequatrovirus T4
-
UV irradiated, contains uracil instead of thymine
-
?
poly(dA)-poly(dT) + H2O
oligonucleotides
Tequatrovirus T4
-
poly(dA)-poly(dT,dU) treated with uracil-DNA glycosylase, endonuclease acts specifically on double-strands
-
?
poly(dA)-poly(dT) + H2O
oligonucleotides
Tequatrovirus T4
-
UV-irradiated
length is less than 15 nucleotides
?
SPO1 DNA
oligonucleotides
Tequatrovirus T4
-
UV irradiated, contains 5-hydroxymethyluracil instead of thymine
-
?
SPO1 DNA
oligonucleotides
Tequatrovirus T4
-
UV irradiated, contains 5-hydroxymethyluracil instead of thymine
-
?
UV-damaged DNA containing cyclobutane pyrimidine dimers
?
Tequatrovirus T4
-
the T4 enzyme, in so called T4N5 liposomes, repairs UV radiation-induced cyclobutane pyrimidine dimers in DNA of epidermal dendritic cells of human skin which reduces the infiltration of macrophages into irradiated human epidermis and leads to memory immunosuppression in humans, overview
-
-
?
UV-damaged DNA containing cyclobutane pyrimidine dimers
?
Tequatrovirus T4
-
the T4 enzyme, in so called T4N5 liposomes, repairs UV radiation-induced cyclobutane pyrimidine dimers in DNA of epidermal dendritic cells of human skin
-
-
?
UV-radiation damaged DNA + H2O
?
-
the enzyme endonucleolytically nicks UV-irradiated DNA and cleaves phosphodiester bonds of the damaged DNAs with broad substrate specificity, higher activity with supercoiled, damaged DNA compared to relaxed, damaged DNA
-
-
?
UV-radiation damaged DNA + H2O
?
-
the enzyme is endonucleolytically nicks UV-irradiated DNA and cleaving phosphodiester bonds of the damaged DNAs with broad substrate specificity, higher activity with supercoiled, damaged DNA compared to relaxed, damaged DNA
-
-
?
UV-radiation damaged PM2 phage DNA + H2O
?
-
the DNA contains thymine glycol
-
-
?
UV-radiation damaged PM2 phage DNA + H2O
?
-
the DNA contains thymine glycol
-
-
?
X174 DNA + H2O
RFII
-
UV-irradiated, denatured DNA: substrate for Py-Py correndonuclease I only
-
?
X174 DNA + H2O
RFII
-
UV irradiated RFI of phage X174 containing apurinic sites
-
?
X174 DNA + H2O
RFII
-
UV irradiated RFI of phage X174 containing apurinic sites
-
?
X174 DNA + H2O
RFII
-
UV irradiated RFI of phage X174 containing apurinic sites
-
?
X174 DNA + H2O
RFII
Tequatrovirus T4
-
UV irradiated RFI of phage X174 containing apurinic sites
RFII, i.e. nicked circular DNA
?
additional information
?
-
-
the enzyme is involved in nucleotide excision repair of damaged DNA removing various types of bulky and distorting DNA adducts induced by UV radiation, process initiation is performed by UvrA, nicking by UvrB, which also recruits UvrC that catalyzes both the 3' and 5' incisions
-
-
?
additional information
?
-
-
the enzyme is involved in nucleotide excision repair of damaged DNA removing various types of bulky and distorting DNA adducts induced by UV radiation, process initiation is performed by UvrA, nicking by UvrB, which also recruits UvrC that catalyzes both the 3' and 5' incisions, substrate recognition and reaction mechanism, overview
-
-
?
additional information
?
-
-
enzyme detects apurinic/apyrimidinic site base paired with adenine
-
-
?
additional information
?
-
-
enzyme detects cyclobutane pyrimidine dimers (DNA lesions) caused by UVA irradiation
-
-
?
additional information
?
-
-
UVA light directly generates a significant number of cyclobutane pyrimidine dimers as detected by a combined action of endonuclease IV and T4 endonuclease V
-
-
?
additional information
?
-
-
not: x-irradiated, psoralen-crosslinked or alkylated DNA
-
-
?
additional information
?
-
-
not: x-irradiated, psoralen-crosslinked or alkylated DNA
-
-
?
additional information
?
-
-
not: substrates containing a trans-syn-I, (6-4), or Dewar thymine dimer, a 5,6-dihydrouracil lesion or an A:G or A:C mismatch
-
-
?
additional information
?
-
-
the enzyme initiates repair of deaminated bases in damaged DNA by making a nucleolytic incision one nucleotide away from the 3' side of the lesion
-
-
?
additional information
?
-
-
substrate specificity, the enzyme performs single-turnover on cleavage of double-stranded or single-stranded deoxynucleotide-containing DNA, the enzyme cleaves deoxyinosine- or deoxyxanthosine-containing DNA with similar nicking activity for the substrates, tight binding to nicking products, deoxyuridine-containing DNA is only slightly acepted as substrate when it is in excess, the active site of the enzyme can accomodate pyrimidine-containing mismatches, the plastic nature of the active site allows the enzyme to enfold both pyrimidine and purine deaminated lesions or base pair mismatches, overview
-
-
?
additional information
?
-
-
the enzyme initiates repair of deaminated bases in damaged DNA by making a nucleolytic incision one nucleotide away from the 3' side of the lesion
-
-
?
additional information
?
-
-
substrate specificity, the enzyme performs single-turnover on cleavage of double-stranded or single-stranded deoxynucleotide-containing DNA, the enzyme cleaves deoxyinosine- or deoxyxanthosine-containing DNA with similar nicking activity for the substrates, tight binding to nicking products, deoxyuridine-containing DNA is only slightly acepted as substrate when it is in excess, the active site of the enzyme can accomodate pyrimidine-containing mismatches, the plastic nature of the active site allows the enzyme to enfold both pyrimidine and purine deaminated lesions or base pair mismatches, overview
-
-
?
additional information
?
-
Tequatrovirus T4
-
not: 5-thyminyl-5,6-dihydrothymine-containing DNA
-
-
?
additional information
?
-
Tequatrovirus T4
-
not: 5-thyminyl-5,6-dihydrothymine-containing DNA
-
-
?
additional information
?
-
Tequatrovirus T4
-
not: DNA exposed to ionizing radiation, methyl methane sulfonate, nitrogen mustard, mitomycin C, 4-nitroquinoline-1-oxide
-
-
?
additional information
?
-
Tequatrovirus T4
-
not: DNA damaged by 4-nitroquinoline-N-oxide or methyl methanesulfonate
-
-
?
additional information
?
-
Tequatrovirus T4
-
psoralen-mediated interstrand cross-links
-
-
?
additional information
?
-
Tequatrovirus T4
enzyme binds to wild-type and damaged DNA
-
-
?
additional information
?
-
Tequatrovirus T4
-
synthesis of cis-syn cyclobutane thymine-uracil dimer, determination of cyclobutane dimer structure by NMR spectroscopy
-
-
?
