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Literature summary for 3.1.25.1 extracted from

  • Friedberg, E.C.; Ganesan, A.K.; Seawell, P.C.
    Purification and properties of a pyrimidine dimer-specific endonuclease from E. coli infected with bacteriophage T4 (1980), Methods Enzymol., 65, 191-201.
    View publication on PubMed
Search for more literature for 3.1.25.1

Inhibitors

Inhibitors Comment Organism Structure
p-chloromercuribenzoate 70-80% inhibition in presence of 0.0004 M Tequatrovirus T4
p-chloromercuriphenylsulfonic acid 20-25% inhibition in presence of 0.0001 M Tequatrovirus T4

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ slight stimulation Tequatrovirus T4
Mn2+ slight stimulation Tequatrovirus T4

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
18000
-
 PAGE, gel filtration Tequatrovirus T4

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
DNA + H2O Tequatrovirus T4
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Tequatrovirus T4
-
-
-

Purification (Commentary)

Purification (Comment) Organism
100-300fold, extensive purification, attempts to concentrate the enzyme preparation by Aquacide II or Sephadex G50 powder results in loss of activity, UV-DNA-cellulose chromatography, hydroxyapatite chromatography, gel filtration through Sephadex or CM-Sephadex as well as sedimentation in sucrose density gradients results in loss of activity if used after phosphocellulose chromatography, enzyme is more stable in fractions containing large amounts of protein like bovine serum albumin Tequatrovirus T4

Source Tissue

Source Tissue Comment Organism Textmining
infected cell T4 infected Tequatrovirus T4
-

Storage Stability

Storage Stability Organism
-12°C, in 12% glycerol, inactivates the enzyme Tequatrovirus T4
4°C, in 50 mM Tris-HCl, pH 7.5, containing 10% ethylene glycol or 3% polyethylene glycol, storage for several months Tequatrovirus T4
freezing, leads to inactivation Tequatrovirus T4

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ColE1 DNA + H2O UV-irradiated, superhelical Tequatrovirus T4 oligonucleotides
-
 ?
DNA + H2O
-
 Tequatrovirus T4 ?
-
 ?
E. coli DNA + H2O single-stranded DNA Tequatrovirus T4 oligonucleotides
-
 ?
E. coli DNA + H2O UV-irradiated dsDNA Tequatrovirus T4 oligonucleotides
-
 ?
glycosylated DNA + H2O UV-irradiated, cleaves also nonglycosylated DNA Tequatrovirus T4 oligonucleotides
-
 ?
additional information not: 5-thyminyl-5,6-dihydrothymine-containing DNA Tequatrovirus T4 ?
-
 ?
additional information not: DNA exposed to ionizing radiation, methyl methane sulfonate, nitrogen mustard, mitomycin C, 4-nitroquinoline-1-oxide Tequatrovirus T4 ?
-
 ?
PBS1 DNA UV irradiated, contains uracil instead of thymine Tequatrovirus T4 oligonucleotides
-
 ?
SPO1 DNA UV irradiated, contains 5-hydroxymethyluracil instead of thymine Tequatrovirus T4 oligonucleotides
-
 ?
superhelical DNA + H2O UV-irradiated, cleaves also linear DNA Tequatrovirus T4 oligonucleotides
-
 ?