Inhibitors | Comment | Organism | Structure |
---|---|---|---|
p-chloromercuribenzoate | 70-80% inhibition in presence of 0.0004 M | Tequatrovirus T4 | |
p-chloromercuriphenylsulfonic acid | 20-25% inhibition in presence of 0.0001 M | Tequatrovirus T4 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | slight stimulation | Tequatrovirus T4 | |
Mn2+ | slight stimulation | Tequatrovirus T4 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
18000 | - |
PAGE, gel filtration | Tequatrovirus T4 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA + H2O | Tequatrovirus T4 | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Tequatrovirus T4 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
100-300fold, extensive purification, attempts to concentrate the enzyme preparation by Aquacide II or Sephadex G50 powder results in loss of activity, UV-DNA-cellulose chromatography, hydroxyapatite chromatography, gel filtration through Sephadex or CM-Sephadex as well as sedimentation in sucrose density gradients results in loss of activity if used after phosphocellulose chromatography, enzyme is more stable in fractions containing large amounts of protein like bovine serum albumin | Tequatrovirus T4 |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
infected cell | T4 infected | Tequatrovirus T4 | - |
Storage Stability | Organism |
---|---|
-12°C, in 12% glycerol, inactivates the enzyme | Tequatrovirus T4 |
4°C, in 50 mM Tris-HCl, pH 7.5, containing 10% ethylene glycol or 3% polyethylene glycol, storage for several months | Tequatrovirus T4 |
freezing, leads to inactivation | Tequatrovirus T4 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ColE1 DNA + H2O | UV-irradiated, superhelical | Tequatrovirus T4 | oligonucleotides | - |
? | |
DNA + H2O | - |
Tequatrovirus T4 | ? | - |
? | |
E. coli DNA + H2O | single-stranded DNA | Tequatrovirus T4 | oligonucleotides | - |
? | |
E. coli DNA + H2O | UV-irradiated dsDNA | Tequatrovirus T4 | oligonucleotides | - |
? | |
glycosylated DNA + H2O | UV-irradiated, cleaves also nonglycosylated DNA | Tequatrovirus T4 | oligonucleotides | - |
? | |
additional information | not: 5-thyminyl-5,6-dihydrothymine-containing DNA | Tequatrovirus T4 | ? | - |
? | |
additional information | not: DNA exposed to ionizing radiation, methyl methane sulfonate, nitrogen mustard, mitomycin C, 4-nitroquinoline-1-oxide | Tequatrovirus T4 | ? | - |
? | |
PBS1 DNA | UV irradiated, contains uracil instead of thymine | Tequatrovirus T4 | oligonucleotides | - |
? | |
SPO1 DNA | UV irradiated, contains 5-hydroxymethyluracil instead of thymine | Tequatrovirus T4 | oligonucleotides | - |
? | |
superhelical DNA + H2O | UV-irradiated, cleaves also linear DNA | Tequatrovirus T4 | oligonucleotides | - |
? |