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Literature summary for 3.1.25.1 extracted from

  • Kim Sang, H.; Lee Jae, Y.; Kim, J.
    Characterization of a wide range base-damage-endonuclease activity of mammalian rpS3 (2005), Biochem. Biophys. Res. Commun., 328, 962-967.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
Mg2+ inhibits the enzyme activity with DNA containing apurinic/apyrimidinic sites, but not with UV-radiation damaged DNA Homo sapiens
Mg2+ inhibits the enzyme activity with DNA containing apurinic/apyrimidinic sites, but not with UV-radiation damaged DNA Mus musculus
additional information the enzyme is sensitive to high salt concentrations Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26700
-
x * 26700, SDS-PAGE Mus musculus
26700
-
x * 26700, SDS-PAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Mus musculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme Mus musculus
recombinant enzyme Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
HeLa cell
-
Homo sapiens
-
MPC-11 cell plasmacytoma cell line Mus musculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
fmol nicks per min Mus musculus
additional information
-
fmol nicks per min Homo sapiens

Storage Stability

Storage Stability Organism
-70°C, purified enzyme, 20 mM Tris-HCl, pH 8.0, 0.1 mM EDTA, 0.01% Triton X-100, 50 mM KCl, 6 months, loss of 55% activity Mus musculus
-70°C, purified enzyme, 20 mM Tris-HCl, pH 8.0, 0.1 mM EDTA, 0.01% Triton X-100, 50 mM KCl, 6 months, loss of 55% activity Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA containing apurinic/apyrimidinic sites + H2O the enzyme is endonucleolytically active on AP sites generated from pyrimidine dimers cleaving phosphodiester bonds of the damaged DNAs with broad specificity Mus musculus ?
-
?
DNA containing apurinic/apyrimidinic sites + H2O the enzyme is endonucleolytically active on AP sites generated from pyrimidine dimers cleaving phosphodiester bonds of the damaged DNAs with broad specificity Homo sapiens ?
-
?
UV-radiation damaged DNA + H2O the enzyme endonucleolytically nicks UV-irradiated DNA and cleaves phosphodiester bonds of the damaged DNAs with broad substrate specificity, higher activity with supercoiled, damaged DNA compared to relaxed, damaged DNA Homo sapiens ?
-
?
UV-radiation damaged DNA + H2O the enzyme is endonucleolytically nicks UV-irradiated DNA and cleaving phosphodiester bonds of the damaged DNAs with broad substrate specificity, higher activity with supercoiled, damaged DNA compared to relaxed, damaged DNA Mus musculus ?
-
?
UV-radiation damaged PM2 phage DNA + H2O the DNA contains thymine glycol Mus musculus ?
-
?
UV-radiation damaged PM2 phage DNA + H2O the DNA contains thymine glycol Homo sapiens ?
-
?

Subunits

Subunits Comment Organism
? x * 26700, SDS-PAGE Mus musculus
? x * 26700, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
ApeI
-
Homo sapiens
endonuclease II
-
Homo sapiens
endonuclease III
-
Mus musculus
ribosomal protein S3
-
Mus musculus
ribosomal protein S3
-
Homo sapiens
rpS3
-
Mus musculus
rpS3
-
Homo sapiens
type I AP endonuclease
-
Mus musculus
type I AP endonuclease
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Mus musculus
37
-
assay at Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
5 min, inactivation Mus musculus
70
-
5 min, inactivation Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 9
-
Mus musculus
8 9
-
Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
8 9 sharp decrease below pH 8.0 Mus musculus
8 9 sharp decrease below pH 8.0 Homo sapiens