Cloned (Comment) | Organism |
---|---|
- |
Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | inhibits the enzyme activity with DNA containing apurinic/apyrimidinic sites, but not with UV-radiation damaged DNA | Homo sapiens | |
Mg2+ | inhibits the enzyme activity with DNA containing apurinic/apyrimidinic sites, but not with UV-radiation damaged DNA | Mus musculus | |
additional information | the enzyme is sensitive to high salt concentrations | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
26700 | - |
x * 26700, SDS-PAGE | Mus musculus |
26700 | - |
x * 26700, SDS-PAGE | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Mus musculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme | Mus musculus |
recombinant enzyme | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
HeLa cell | - |
Homo sapiens | - |
MPC-11 cell | plasmacytoma cell line | Mus musculus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
fmol nicks per min | Mus musculus |
additional information | - |
fmol nicks per min | Homo sapiens |
Storage Stability | Organism |
---|---|
-70°C, purified enzyme, 20 mM Tris-HCl, pH 8.0, 0.1 mM EDTA, 0.01% Triton X-100, 50 mM KCl, 6 months, loss of 55% activity | Mus musculus |
-70°C, purified enzyme, 20 mM Tris-HCl, pH 8.0, 0.1 mM EDTA, 0.01% Triton X-100, 50 mM KCl, 6 months, loss of 55% activity | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA containing apurinic/apyrimidinic sites + H2O | the enzyme is endonucleolytically active on AP sites generated from pyrimidine dimers cleaving phosphodiester bonds of the damaged DNAs with broad specificity | Mus musculus | ? | - |
? | |
DNA containing apurinic/apyrimidinic sites + H2O | the enzyme is endonucleolytically active on AP sites generated from pyrimidine dimers cleaving phosphodiester bonds of the damaged DNAs with broad specificity | Homo sapiens | ? | - |
? | |
UV-radiation damaged DNA + H2O | the enzyme endonucleolytically nicks UV-irradiated DNA and cleaves phosphodiester bonds of the damaged DNAs with broad substrate specificity, higher activity with supercoiled, damaged DNA compared to relaxed, damaged DNA | Homo sapiens | ? | - |
? | |
UV-radiation damaged DNA + H2O | the enzyme is endonucleolytically nicks UV-irradiated DNA and cleaving phosphodiester bonds of the damaged DNAs with broad substrate specificity, higher activity with supercoiled, damaged DNA compared to relaxed, damaged DNA | Mus musculus | ? | - |
? | |
UV-radiation damaged PM2 phage DNA + H2O | the DNA contains thymine glycol | Mus musculus | ? | - |
? | |
UV-radiation damaged PM2 phage DNA + H2O | the DNA contains thymine glycol | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 26700, SDS-PAGE | Mus musculus |
? | x * 26700, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
ApeI | - |
Homo sapiens |
endonuclease II | - |
Homo sapiens |
endonuclease III | - |
Mus musculus |
ribosomal protein S3 | - |
Mus musculus |
ribosomal protein S3 | - |
Homo sapiens |
rpS3 | - |
Mus musculus |
rpS3 | - |
Homo sapiens |
type I AP endonuclease | - |
Mus musculus |
type I AP endonuclease | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Mus musculus |
37 | - |
assay at | Homo sapiens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
5 min, inactivation | Mus musculus |
70 | - |
5 min, inactivation | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 9 | - |
Mus musculus |
8 | 9 | - |
Homo sapiens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8 | 9 | sharp decrease below pH 8.0 | Mus musculus |
8 | 9 | sharp decrease below pH 8.0 | Homo sapiens |