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Literature summary for 3.1.25.1 extracted from

  • Nakatsu, Y.; Nakabeppu, Y.; Sekiguchi, M.
    Action of T4 endonuclease V on polydeoxyribonucleotides with apyrimidinic or apurinic sites (1982), J. Biochem., 91, 2057-2065.
    View publication on PubMed
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Inhibitors

Inhibitors Comment Organism Structure
Mg2+ in presence of 10 mM MgCl2: 10% of the activity observed in presence of 10 mM EDTA Tequatrovirus T4
NaBH4 inhibits degradation of the glycosylase product, reduces aldehyde moiety of the apyrimidinic sites to alcohol which results in resistance to endonuclease Tequatrovirus T4

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
DNA + H2O Tequatrovirus T4
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Tequatrovirus T4
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Tequatrovirus T4

Reaction

Reaction Comment Organism Reaction ID
endonucleolytic cleavage near pyrimidine dimers to products with 5'-phosphate cleavage on the 3'-side of the apyrimidinic site Tequatrovirus T4
a
endonucleolytic cleavage near pyrimidine dimers to products with 5'-phosphate 2-step mechanism, pyrimidine dimer-DNA glycosylase and an apurinic/apyrimidinic endonuclease Tequatrovirus T4
a

Source Tissue

Source Tissue Comment Organism Textmining
infected cell T4 infected Tequatrovirus T4
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA + H2O
-
 Tequatrovirus T4 ?
-
 ?
poly(dA)-poly(dT) + H2O poly(dA)-poly(dT,dU) treated with uracil-DNA glycosylase, endonuclease acts specifically on double-strands Tequatrovirus T4 oligonucleotides
-
 ?
X174 DNA + H2O UV irradiated RFI of phage X174 containing apurinic sites Tequatrovirus T4 RFII RFII, i.e. nicked circular DNA ?