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Literature summary for 3.1.25.1 extracted from

  • Piersen, C.E.; Prince, M.A.; Augustine, M.L.; Dodson, M.L.; Lloyd, R.S.
    Purification and cloning of Micrococcus luteus ultraviolet endonuclease, an N-glycosylase/abasic lyase that proceeds via an imino enzyme-DNA intermediate (1995), J. Biol. Chem., 270, 23475-23484.
    View publication on PubMed
Search for more literature for 3.1.25.1

Cloned(Commentary)

Cloned (Comment) Organism
-
 Micrococcus luteus

Inhibitors

Inhibitors Comment Organism Structure
NaBH4 complexes are formed at a concentration of more than 10 mM NaBH4; leads to a covalently linked enzyme-substrate product Micrococcus luteus
NaCN 50% inhibition occurs in presence of around 6 mM NaCN; reacts with imino intermediate Micrococcus luteus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29310 30340 cloning identified an open reading frame with two stop codons encoding for two enzymes with these calculated masses Micrococcus luteus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
DNA + H2O Micrococcus luteus
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Micrococcus luteus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Micrococcus luteus

Reaction

Reaction Comment Organism Reaction ID
endonucleolytic cleavage near pyrimidine dimers to products with 5'-phosphate specific for cis-syn cyclobutane pyrimidine dimers Micrococcus luteus
a
endonucleolytic cleavage near pyrimidine dimers to products with 5'-phosphate processive Micrococcus luteus
a
endonucleolytic cleavage near pyrimidine dimers to products with 5'-phosphate beta-elimination mechanism, imino intermediate Micrococcus luteus
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA + H2O
-
 Micrococcus luteus ?
-
 ?
additional information not: substrates containing a trans-syn-I, (6-4), or Dewar thymine dimer, a 5,6-dihydrouracil lesion or an A:G or A:C mismatch Micrococcus luteus ?
-
 ?
oligodeoxyribonucleotides dimer containing 49mer Micrococcus luteus oligonucleotides
-
 ?