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Literature summary for 3.1.25.1 extracted from

  • Riazuddin, S.
    Purification and properties of pyrimidine dimer specific endonucleases from Micrococcus luteus (1980), Methods Enzymol., 65, 185-191.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
Tris-HCl optimum for Py-Py correndonuclease I: 10 mM supplemented with 50 mM NaCl or potassium phosphate Micrococcus luteus
Tris-HCl optimum for Py-Py correndonuclease II: 10 mM Micrococcus luteus

Inhibitors

Inhibitors Comment Organism Structure
KCl Py-Py correndonuclease II loses 75% of activity in presence of 100 mM Micrococcus luteus
Mg2+ Py-Py correndonuclease II loses 75% of activity in presence of 15 mM Micrococcus luteus
NaCl Py-Py correndonuclease II loses 75% of activity in presence of 100 mM Micrococcus luteus

Metals/Ions

Metals/Ions Comment Organism Structure
potassium phosphate activating, optimum for Py-Py correndonuclease I: 50 mM supplemented with 10 mM Tris-HCl Micrococcus luteus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
DNA + H2O Micrococcus luteus
-
?
-
?

Organism

Organism UniProt Comment Textmining
Micrococcus luteus
-
-
-

Reaction

Reaction Comment Organism Reaction ID
endonucleolytic cleavage near pyrimidine dimers to products with 5'-phosphate minimum chain length: longer than 10 nucleotides Micrococcus luteus
endonucleolytic cleavage near pyrimidine dimers to products with 5'-phosphate Py-Py correndonuclease I creates a terminus which is more single-stranded in character than that produced by Py-Py correndonuclease II Micrococcus luteus
endonucleolytic cleavage near pyrimidine dimers to products with 5'-phosphate dsDNA is an effective substrate vor Py-Py correndonucleases I and II, only correndonuclease I can degrade ssDNA Micrococcus luteus
endonucleolytic cleavage near pyrimidine dimers to products with 5'-phosphate for DNA repair the combined action of Py-Py correndonuclease I and II is necessary Micrococcus luteus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Micrococcus luteus

Storage Stability

Storage Stability Organism
-20°C, in 50% glycerol, complete inactivation in 3-4 weeks Micrococcus luteus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA + H2O
-
Micrococcus luteus ?
-
?
additional information not: OsO4-treated DNA Micrococcus luteus ?
-
?
additional information not: x-irradiated, psoralen-crosslinked or alkylated DNA Micrococcus luteus ?
-
?
X174 DNA + H2O UV irradiated RFI of phage X174 containing apurinic sites Micrococcus luteus RFII
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
Py-Py correndonuclease I loses 20% of activity after 5 min, Py-Py correndonuclease II loses more than 75% of activity after 5 min Micrococcus luteus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 7.4 Py-Py correndonucleases I and II Micrococcus luteus