Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Tris-HCl | optimum for Py-Py correndonuclease I: 10 mM supplemented with 50 mM NaCl or potassium phosphate | Micrococcus luteus | |
Tris-HCl | optimum for Py-Py correndonuclease II: 10 mM | Micrococcus luteus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
KCl | Py-Py correndonuclease II loses 75% of activity in presence of 100 mM | Micrococcus luteus | |
Mg2+ | Py-Py correndonuclease II loses 75% of activity in presence of 15 mM | Micrococcus luteus | |
NaCl | Py-Py correndonuclease II loses 75% of activity in presence of 100 mM | Micrococcus luteus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
potassium phosphate | activating, optimum for Py-Py correndonuclease I: 50 mM supplemented with 10 mM Tris-HCl | Micrococcus luteus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA + H2O | Micrococcus luteus | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Micrococcus luteus | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
endonucleolytic cleavage near pyrimidine dimers to products with 5'-phosphate | minimum chain length: longer than 10 nucleotides | Micrococcus luteus | |
endonucleolytic cleavage near pyrimidine dimers to products with 5'-phosphate | Py-Py correndonuclease I creates a terminus which is more single-stranded in character than that produced by Py-Py correndonuclease II | Micrococcus luteus | |
endonucleolytic cleavage near pyrimidine dimers to products with 5'-phosphate | dsDNA is an effective substrate vor Py-Py correndonucleases I and II, only correndonuclease I can degrade ssDNA | Micrococcus luteus | |
endonucleolytic cleavage near pyrimidine dimers to products with 5'-phosphate | for DNA repair the combined action of Py-Py correndonuclease I and II is necessary | Micrococcus luteus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Micrococcus luteus |
Storage Stability | Organism |
---|---|
-20°C, in 50% glycerol, complete inactivation in 3-4 weeks | Micrococcus luteus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA + H2O | - |
Micrococcus luteus | ? | - |
? | |
additional information | not: OsO4-treated DNA | Micrococcus luteus | ? | - |
? | |
additional information | not: x-irradiated, psoralen-crosslinked or alkylated DNA | Micrococcus luteus | ? | - |
? | |
X174 DNA + H2O | UV irradiated RFI of phage X174 containing apurinic sites | Micrococcus luteus | RFII | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
Py-Py correndonuclease I loses 20% of activity after 5 min, Py-Py correndonuclease II loses more than 75% of activity after 5 min | Micrococcus luteus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 7.4 | Py-Py correndonucleases I and II | Micrococcus luteus |