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3.1.25.1: deoxyribonuclease (pyrimidine dimer)

This is an abbreviated version!
For detailed information about deoxyribonuclease (pyrimidine dimer), go to the full flat file.

Word Map on EC 3.1.25.1

Reaction

endonucleolytic cleavage near pyrimidine dimers to products with 5'-phosphate =

Synonyms

ApeI, bacteriophage T4 endodeoxyribonuclease V, bacteriophage T4 endonuclease V, correndonuclease I, correndonuclease II, endodeoxyribonuclease (pyrimidine dimer), endonuclease II, endonuclease III, endonuclease V, Escherichia coli UV endonuclease, excision endonuclease UvrABC, excision nuclease UvrABC, More, nuclease V, bacteriophage T4 endodeoxyribonuclease V, nuclease, Escherichia coli UV-endodeoxyribonuclease, Py-Py-correndonuclease, ribosomal protein S3, rpS3, T4 Endo V, T4 endonuclease V, T4N5, type I AP endonuclease, UvrA, UvrABC nuclease, UvrB, UvrC

ECTree

     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.25 Site-specific endodeoxyribonucleases that are specific for altered bases
                3.1.25.1 deoxyribonuclease (pyrimidine dimer)

Purification

Purification on EC 3.1.25.1 - deoxyribonuclease (pyrimidine dimer)

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
100-300fold, extensive purification, attempts to concentrate the enzyme preparation by Aquacide II or Sephadex G50 powder results in loss of activity, UV-DNA-cellulose chromatography, hydroxyapatite chromatography, gel filtration through Sephadex or CM-Sephadex as well as sedimentation in sucrose density gradients results in loss of activity if used after phosphocellulose chromatography, enzyme is more stable in fractions containing large amounts of protein like bovine serum albumin
Tequatrovirus T4
-
3500fold
Tequatrovirus T4
-
4100fold, to homogeneity
Tequatrovirus T4
-
native enzyme
-
recombinant enzyme
-
recombinant from Escherichia coli
Tequatrovirus T4
-
recombinant UvrA, UvrB, and UvrC
-
recombinant wild-type and mutant C-terminally His-tagged enzymes from Escherichia coli strain BL21(DE3) by nickel chelating affinity chromatography to homogeneity
-
T127M
Tequatrovirus T4
-
to homogeneity
Tequatrovirus T4
-
WT-enzyme and W128S
Tequatrovirus T4
-
WT-enzyme: 300fold, enzyme from mutant strain F794: partially purified
Tequatrovirus T4
-