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3.1.11.1: exodeoxyribonuclease I

This is an abbreviated version!
For detailed information about exodeoxyribonuclease I, go to the full flat file.

Word Map on EC 3.1.11.1

Reaction

Exonucleolytic cleavage in the 3'- to 5'- direction to yield nucleoside 5'-phosphates =

Synonyms

3'->5'exonuclease, 3'-to-5' exonuclease, 3'5' exonuclease, 30–50 ssExo, DNA deoxyribophosphodiesterase, DNA polymerase D, DNA polymerase I, dRPase, E. coli exonuclease I, EC 3.1.4.25, Escherichia coli exonuclease I, Exo I, Exo1, EXO1b, ExoA, ExoI, exonuclease 1, exonuclease 1b, exonuclease I, exonuxlease 1, hEXO1, More, OsEXO1, PF2046, PfuExo I, PhoExo I, phosphodiesterase, Pol, Pol BI, POlB1, polD, SbcB15, single-strand DNA 3'-5' exonuclease, single-strand-specific 3'-5' exonuclease, single-stranded DNA-specific 3'-5' exonuclease I, Sso polB1, SSO0552, tthb178, WRN

ECTree

     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.11 Exodeoxyribonucleases producing 5′-phosphomonoesters
                3.1.11.1 exodeoxyribonuclease I

KM Value

KM Value on EC 3.1.11.1 - exodeoxyribonuclease I

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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.1
oligonucleotide (polydA)4
-
inverse dependence with polymer size
-
0.04
oligonucleotide (polydT)(polydA)(polydT)(polydA)
-
-
-
0.016
single-stranded DNA
-
at 37°C, in 20 mM Tris-HCl buffer, pH 8.0, 100 mM NaCl, 3 mM MgCl2, 1 mM 2-mercaptoethanol, 0.1 g/l bovine serum albumin, 10% (v/v) glycerol, 5% (v/v) dimethyl sulfoxide
0.28 - 0.48
ssDNA 10 mer
-
0.35 - 0.44
ssDNA 21 mer
-
0.26 - 0.29
ssDNA 28 mer
-
0.24 - 0.32
ssDNA 40 mer
-
0.13 - 0.16
ssDNA 50 mer
-
additional information
additional information
-
kinetics of the enzyme located in streptavidin-coated microspheres with biotinylated DNA substrate and Mg2+ cofactor
-