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3.1.11.1: exodeoxyribonuclease I

This is an abbreviated version!
For detailed information about exodeoxyribonuclease I, go to the full flat file.

Word Map on EC 3.1.11.1

Reaction

Exonucleolytic cleavage in the 3'- to 5'- direction to yield nucleoside 5'-phosphates =

Synonyms

3'->5'exonuclease, 3'-to-5' exonuclease, 3'5' exonuclease, 30–50 ssExo, DNA deoxyribophosphodiesterase, DNA polymerase D, DNA polymerase I, dRPase, E. coli exonuclease I, EC 3.1.4.25, Escherichia coli exonuclease I, Exo I, Exo1, EXO1b, ExoA, ExoI, exonuclease 1, exonuclease 1b, exonuclease I, exonuxlease 1, hEXO1, More, OsEXO1, PF2046, PfuExo I, PhoExo I, phosphodiesterase, Pol, Pol BI, POlB1, polD, SbcB15, single-strand DNA 3'-5' exonuclease, single-strand-specific 3'-5' exonuclease, single-stranded DNA-specific 3'-5' exonuclease I, Sso polB1, SSO0552, tthb178, WRN

ECTree

     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.11 Exodeoxyribonucleases producing 5′-phosphomonoesters
                3.1.11.1 exodeoxyribonuclease I

Cloned

Cloned on EC 3.1.11.1 - exodeoxyribonuclease I

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
construction of a fusion gene encoding OsEXO1-sGFP fusion protein whose expression is regulated by the cauliflower mosaic virus 35S promoter. The fusion gene is transferred into onion epidermal cells by particle bombardment. When it is transiently expressed in onion cells, the green fluorescence is localized exclusively to the nucleus
deletion mutants of sbcB, xthA and recBrecC genes
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expressed in Escherichia coli BH110(DE3) cells
expressed in insect Sf9 cells
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expression in Escherichia coli
expression of hEXO1 in Spodoptera frugiperda Sf9 insect cells using the baculovirus vector system
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expression of proteins from large subunit DP2(1-100), large subunit DP2(792-1163), and small subunit DP1(1-200)
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into plasmid pcDNA3.1A(-), expressing a COOH-terminal His-Myc-tagged EXO1 protein
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murine NIH3T3 cells are transiently cotransfected with plasmids expressing CFP-PCNA and YFP-hEXO1, expression of wild-type and mutant N-terminal YFP-tagged hEXO1 fusion proteins in Escherichia coli strain BL21(DE3), in vitro transcription translation of hEXO1, plasmid pcDNA3.1A/myc-His(-)A-hEXO1 expressing COOH-terminal his6 and myc epitope tagged hEXO1
mutation of the xon gene leads to a temperature sensitive enzyme
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overexpression in Escherichia coli
overexpression of Escherichia coli exonuclease I in Deinococcus inhibits DNA double-strand break repair
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produced as inclusion bodies in Escherichia coli
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recombinant expression of the His-tagged enzyme in Escherichia coli strain BL21(AI)
stable expression of GFP-tagged EXO1 in U2OS cells
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transgenic Deinococcus cells expressing exonuclease I functions of Escherichia coli show significant reduction in gamma radiation radioresistance, while the resistance to far-UV and hydrogen peroxide remains unaffected. The overexpression of Escherichia coli exonuclease I in Deinococcus inhibits DNA double-strand break repair
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