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3.1.11.1: exodeoxyribonuclease I

This is an abbreviated version!
For detailed information about exodeoxyribonuclease I, go to the full flat file.

Word Map on EC 3.1.11.1

Reaction

Exonucleolytic cleavage in the 3'- to 5'- direction to yield nucleoside 5'-phosphates =

Synonyms

3'->5'exonuclease, 3'-to-5' exonuclease, 3'5' exonuclease, 30–50 ssExo, DNA deoxyribophosphodiesterase, DNA polymerase D, DNA polymerase I, dRPase, E. coli exonuclease I, EC 3.1.4.25, Escherichia coli exonuclease I, Exo I, Exo1, EXO1b, ExoA, ExoI, exonuclease 1, exonuclease 1b, exonuclease I, exonuxlease 1, hEXO1, More, OsEXO1, PF2046, PfuExo I, PhoExo I, phosphodiesterase, Pol, Pol BI, POlB1, polD, SbcB15, single-strand DNA 3'-5' exonuclease, single-strand-specific 3'-5' exonuclease, single-stranded DNA-specific 3'-5' exonuclease I, Sso polB1, SSO0552, tthb178, WRN

ECTree

     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.11 Exodeoxyribonucleases producing 5′-phosphomonoesters
                3.1.11.1 exodeoxyribonuclease I

Purification

Purification on EC 3.1.11.1 - exodeoxyribonuclease I

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
near homogeneity by chromatography steps
-
Ni-NTA agarose resin column chromatography
overexpression in Escherichia coli, chromatography techniques, near homogeneity
-
Q-Sepharose column chromatography and HiTrap-heparin column chromatography
recombinant His-tagged enzyme from Escherichia coli strain BL21(AI) by nickel affinity chromatography and ultrafiltration
Talon column chromatography and heparin column chromatography
-
to homogeneitiy by affinity chromatography
-
to homogeneity by chromatography steps
-