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Literature summary for 3.1.11.1 extracted from

  • Lu, D.; Windsor, M.A.; Gellman, S.H.; Keck, J.L.
    Peptide inhibitors identify roles for SSB C-terminal residues in SSB/exonuclease I complex formation (2009), Biochemistry, 48, 6764-6771.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.11.1

Activating Compound

Activating Compound Comment Organism Structure
additional information ExoI enzymatic activity is stimulated by single-stranded DNA-binding protein, whereby the hydrophobic C-terminal residues play critical roles in ExoI binding Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
single-stranded DNA-binding protein deleting the N-terminal most Met from the C-terminus of single-stranded DNA-binding protein sequence has a negligible effect on apparent binding to ExoI. The C-terminus of single-stranded DNA-binding proteins abrogate single-stranded DNA-binding protein stimulation of ExoI activity through a competitive inhibition mechanism, the peptides can disrupt ExoI/single-stranded DNA-binding protein/single-stranded DNA ternary complexes. C-terminus of single-stranded DNA-binding protein inhibition is dose-dependent, requiring ca. 0.001 mM peptide to achieve 50% inhibition and ca. 0.01 mM to reduce ExoI activity to C-terminus of single-stranded DNA-binding protein-free levels. Addition of up to 0.1 mM of C-terminus of single-stranded DNA-binding protein does not inhibit ExoI activity to levels below that of ExoI with free single-stranded DNA, and addition of the peptide to single-stranded DNA-binding-protein free reactions has no measurable effect on ExoI nuclease activity Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.016
-
 single-stranded DNA at 37°C, in 20 mM Tris-HCl buffer, pH 8.0, 100 mM NaCl, 3 mM MgCl2, 1 mM 2-mercaptoethanol, 0.1 g/l bovine serum albumin, 10% (v/v) glycerol, 5% (v/v) dimethyl sulfoxide Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
single-stranded DNA + H2O
-
 Escherichia coli ?
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
115.5
-
 single-stranded DNA at 37°C, in 20 mM Tris-HCl buffer, pH 8.0, 100 mM NaCl, 3 mM MgCl2, 1 mM 2-mercaptoethanol, 0.1 g/l bovine serum albumin, 10% (v/v) glycerol, 5% (v/v) dimethyl sulfoxide Escherichia coli

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0043
-
 peptide inhibitor with N-terminal truncation WDFDDDIPF, at 22°C in 20 mM Tris-HCl buffer, pH 8.0, 100 mM NaCl, 1 mM MgCl2, 1 mM 2-mercaptoethanol, 0.1 g/l bovine serum albumin, 4% (v/v) glycerol, 1% (v/v) dimethyl sulfoxide Escherichia coli single-stranded DNA-binding protein
0.0052
-
 wild type peptide inhibitor, at 22°C in 20 mM Tris-HCl buffer, pH 8.0, 100 mM NaCl, 1 mM MgCl2, 1 mM 2-mercaptoethanol, 0.1 g/l bovine serum albumin, 4% (v/v) glycerol, 1% (v/v) dimethyl sulfoxide Escherichia coli single-stranded DNA-binding protein
0.0067
-
 peptide inhibitor with neutralizing modification WMDFDADIPF, at 22°C in 20 mM Tris-HCl buffer, pH 8.0, 100 mM NaCl, 1 mM MgCl2, 1 mM 2-mercaptoethanol, 0.1 g/l bovine serum albumin, 4% (v/v) glycerol, 1% (v/v) dimethyl sulfoxide Escherichia coli single-stranded DNA-binding protein
0.0085
-
 peptide inhibitor with neutralizing modification WMDFADDIPF, at 22°C in 20 mM Tris-HCl buffer, pH 8.0, 100 mM NaCl, 1 mM MgCl2, 1 mM 2-mercaptoethanol, 0.1 g/l bovine serum albumin, 4% (v/v) glycerol, 1% (v/v) dimethyl sulfoxide Escherichia coli single-stranded DNA-binding protein
0.0093
-
 peptide inhibitor with neutralizing modification WMDFDDAIPF, at 22°C in 20 mM Tris-HCl buffer, pH 8.0, 100 mM NaCl, 1 mM MgCl2, 1 mM 2-mercaptoethanol, 0.1 g/l bovine serum albumin, 4% (v/v) glycerol, 1% (v/v) dimethyl sulfoxide Escherichia coli single-stranded DNA-binding protein
0.0116
-
 peptide inhibitor with neutralizing modification WMAFDDDIPF, at 22°C in 20 mM Tris-HCl buffer, pH 8.0, 100 mM NaCl, 1 mM MgCl2, 1 mM 2-mercaptoethanol, 0.1 g/l bovine serum albumin, 4% (v/v) glycerol, 1% (v/v) dimethyl sulfoxide Escherichia coli single-stranded DNA-binding protein
0.0121
-
 peptide inhibitor with N-terminal truncation WFDDDIPF, at 22°C in 20 mM Tris-HCl buffer, pH 8.0, 100 mM NaCl, 1 mM MgCl2, 1 mM 2-mercaptoethanol, 0.1 g/l bovine serum albumin, 4% (v/v) glycerol, 1% (v/v) dimethyl sulfoxide Escherichia coli single-stranded DNA-binding protein
0.0253
-
 peptide inhibitor with N-terminal truncation WDDDIPF, at 22°C in 20 mM Tris-HCl buffer, pH 8.0, 100 mM NaCl, 1 mM MgCl2, 1 mM 2-mercaptoethanol, 0.1 g/l bovine serum albumin, 4% (v/v) glycerol, 1% (v/v) dimethyl sulfoxide Escherichia coli single-stranded DNA-binding protein
0.0335
-
 peptide inhibitor with N-terminal truncation WDDIPF, at 22°C in 20 mM Tris-HCl buffer, pH 8.0, 100 mM NaCl, 1 mM MgCl2, 1 mM 2-mercaptoethanol, 0.1 g/l bovine serum albumin, 4% (v/v) glycerol, 1% (v/v) dimethyl sulfoxide Escherichia coli single-stranded DNA-binding protein
0.0529
-
 peptide inhibitor with C-terminal modification/truncation WMDFDDDISF, at 22°C in 20 mM Tris-HCl buffer, pH 8.0, 100 mM NaCl, 1 mM MgCl2, 1 mM 2-mercaptoethanol, 0.1 g/l bovine serum albumin, 4% (v/v) glycerol, 1% (v/v) dimethyl sulfoxide Escherichia coli single-stranded DNA-binding protein
0.0791
-
 peptide inhibitor with N-terminal truncation WDIPF, at 22°C in 20 mM Tris-HCl buffer, pH 8.0, 100 mM NaCl, 1 mM MgCl2, 1 mM 2-mercaptoethanol, 0.1 g/l bovine serum albumin, 4% (v/v) glycerol, 1% (v/v) dimethyl sulfoxide Escherichia coli single-stranded DNA-binding protein
0.169
-
 peptide inhibitor with neutralizing modification WMAFAAAIPF, at 22°C in 20 mM Tris-HCl buffer, pH 8.0, 100 mM NaCl, 1 mM MgCl2, 1 mM 2-mercaptoethanol, 0.1 g/l bovine serum albumin, 4% (v/v) glycerol, 1% (v/v) dimethyl sulfoxide Escherichia coli single-stranded DNA-binding protein
0.252
-
 peptide inhibitor with N-terminal truncation WIPF, at 22°C in 20 mM Tris-HCl buffer, pH 8.0, 100 mM NaCl, 1 mM MgCl2, 1 mM 2-mercaptoethanol, 0.1 g/l bovine serum albumin, 4% (v/v) glycerol, 1% (v/v) dimethyl sulfoxide Escherichia coli single-stranded DNA-binding protein
0.382
-
 peptide inhibitor with C-terminal modification/truncation WMDFDDDIPY, at 22°C in 20 mM Tris-HCl buffer, pH 8.0, 100 mM NaCl, 1 mM MgCl2, 1 mM 2-mercaptoethanol, 0.1 g/l bovine serum albumin, 4% (v/v) glycerol, 1% (v/v) dimethyl sulfoxide Escherichia coli single-stranded DNA-binding protein
1
-
 peptide inhibitor with C-terminal modification/truncation WMDFDDD, at 22°C in 20 mM Tris-HCl buffer, pH 8.0, 100 mM NaCl, 1 mM MgCl2, 1 mM 2-mercaptoethanol, 0.1 g/l bovine serum albumin, 4% (v/v) glycerol, 1% (v/v) dimethyl sulfoxide Escherichia coli single-stranded DNA-binding protein
1
-
 peptide inhibitor with C-terminal modification/truncation WMDFDDDI, at 22°C in 20 mM Tris-HCl buffer, pH 8.0, 100 mM NaCl, 1 mM MgCl2, 1 mM 2-mercaptoethanol, 0.1 g/l bovine serum albumin, 4% (v/v) glycerol, 1% (v/v) dimethyl sulfoxide Escherichia coli single-stranded DNA-binding protein
1
-
 peptide inhibitor with C-terminal modification/truncation WMDFDDDIP, at 22°C in 20 mM Tris-HCl buffer, pH 8.0, 100 mM NaCl, 1 mM MgCl2, 1 mM 2-mercaptoethanol, 0.1 g/l bovine serum albumin, 4% (v/v) glycerol, 1% (v/v) dimethyl sulfoxide Escherichia coli single-stranded DNA-binding protein