1.1.1.8: glycerol-3-phosphate dehydrogenase (NAD+)

This is an abbreviated version, for detailed information about glycerol-3-phosphate dehydrogenase (NAD+), go to the full flat file.

Reaction

sn-glycerol 3-phosphate
+
acceptor
=
glycerone phosphate
+
reduced acceptor

Synonyms

(NAD+)-dependent G3pdh, (NAD+)-dependent glycerol-3-phosphate dehydrogenase, alpha glycerophosphate dehydrogenase, alpha-glycerol phosphate dehydrogenase (NAD), alpha-glycerophosphate dehydrogenase (NAD), Cagpd1, Cagpd2, cG3PDH, CgGPD, cGPdH, cytoplasmic NAD-dependent glycerol-3-phosphate dehydrogenase, dehydrogenase, glycerol phosphate, DhGPD1, G-3-P dehydrogenase, G3P dehydrogenase, G3PD, G3PDH, GDM, GDP2, GLPD, glycerol 1-phosphate dehydrogenase, glycerol 3-phosphate dehydrogenase, glycerol 3-phosphate dehydrogenase 1, glycerol phosphate dehydrogenase (NAD), glycerol-3-phosphate dehydrogenase, glycerol-3-phosphate dehydrogenase 1, glycerophosphate dehydrogenase (NAD), GPD, GPD1, GPDH, GPDH-1, GPDH-2, GPDH1, GPDH2, GPDH: NAD+ 2 oxidorreductase, GPDHc1, hydroglycerophosphate dehydrogenase, L-alpha-glycerol phosphate dehydrogenase, L-alpha-glycerophosphate dehydrogenase, L-glycerol 3-phosphate dehydrogenase, L-glycerol phosphate dehydrogenase, L-glycerophosphate dehydrogenase, mGPDH, NAD+-dependent glycerol 3-phosphate dehydrogenase, NAD+-dependent glycerol-3-phosphate dehydrogenase, NAD+-GPDH, NAD-alpha-glycerophosphate dehydrogenase, NAD-dependent cG3PDH, NAD-dependent glycerol phosphate dehydrogenase, NAD-dependent glycerol-3-phosphate dehydrogenase, NAD-L-glycerol-3-phosphate dehydrogenase, NAD-linked glycerol 3-phosphate dehydrogenase, NADH-dihydroxyacetone phosphate reductase

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.8 glycerol-3-phosphate dehydrogenase (NAD+)

Storage Stability

Storage Stability on EC 1.1.1.8 - glycerol-3-phosphate dehydrogenase (NAD+)

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20-0°C, stable in 75% ammonium sulfate
-
-20°C, 50% glycerol, retains activity for several months
-
-20°C, in 0.1 M Tris-HCl, 2 mM 2-mercaptoethanol, pH 7.0, 50% polyethylene glycol 2000, 5 mM NADH or dihydroxyacetone phosphate, unstable
-
-20°C, stable as (NH4)2SO4-precipitate
-
-20°C, stable for at least 2 months
-
-80°C, in 0.1 M Tris-HCl, 2 mM 2-mercaptoethanol, pH 7.0, 50% polyethylene glycol 2000, 5 mM NADH or dihydroxyacetone phosphate, unstable
-
0°C, 30 mM Tris-H2SO4, 2.5 mM Na2EDTA, 5 mM dithiothreitol, 0,05% NaN3 (pH 8, 4°C), 0.1 mM phenylmethylsulfonylfluoride, 15-17.5% w/v poly(ethylene glycol) 4000, after six days loss of activity insignificant
-
0°C, crude extract stable for 6 days in presence of polyethylene glycol
-
0°C, t1/2: 50 min
-
2°C, crystals stable at least a month in 0.1 M Tris buffer, pH 7.6 with added EDTA, dithiothreitol and ammonium sulfate
-
4°C, 1 M Tris-HCl, 2 mM 2-mercaptoethanol, pH 7.0, eluted from Sephadex G-100 column, 90% activity remained after several weeks
-
4°C, 20% v/v, glycerol, purified stable
4°C, dilute solution, pH 5.8, stable for weeks
-
4°C, purified and dilute preparation stable for several days in presence of NADH
-
4°C, stable
4°C, stable for several weeks in 1 M Tris/HCl buffer with 2-mercaptoethanol, pH 7.0
-
4°C, t1/2: 6 h in absence of EDTA and polyethyleneglycol
-
5°C, isozyme 1: stable for over a month in 10 mM sodium phosphate buffer, pH 6.5, with added EDTA, dithiothreitol and NADH, isozyme 3: 50% loss of activity under the same conditions
-
crystallized, in ammonium sulfate stable for months
-
crystals, 2°C in 0.1M Tris, 10 mM EDTA, 2 mM dithiothreitol, pH 7.6, (NH4)2SO4 in final concentration of 1.7 M
-