1.1.1.8: glycerol-3-phosphate dehydrogenase (NAD+)

This is an abbreviated version, for detailed information about glycerol-3-phosphate dehydrogenase (NAD+), go to the full flat file.

Reaction

sn-glycerol 3-phosphate
+
acceptor
=
glycerone phosphate
+
reduced acceptor

Synonyms

(NAD+)-dependent G3pdh, (NAD+)-dependent glycerol-3-phosphate dehydrogenase, alpha glycerophosphate dehydrogenase, alpha-glycerol phosphate dehydrogenase (NAD), alpha-glycerophosphate dehydrogenase (NAD), Cagpd1, Cagpd2, cG3PDH, CgGPD, cGPdH, cytoplasmic NAD-dependent glycerol-3-phosphate dehydrogenase, dehydrogenase, glycerol phosphate, DhGPD1, G-3-P dehydrogenase, G3P dehydrogenase, G3PD, G3PDH, GDM, GDP2, GLPD, glycerol 1-phosphate dehydrogenase, glycerol 3-phosphate dehydrogenase, glycerol 3-phosphate dehydrogenase 1, glycerol phosphate dehydrogenase (NAD), glycerol-3-phosphate dehydrogenase, glycerol-3-phosphate dehydrogenase 1, glycerophosphate dehydrogenase (NAD), GPD, GPD1, GPDH, GPDH-1, GPDH-2, GPDH1, GPDH2, GPDH: NAD+ 2 oxidorreductase, GPDHc1, hydroglycerophosphate dehydrogenase, L-alpha-glycerol phosphate dehydrogenase, L-alpha-glycerophosphate dehydrogenase, L-glycerol 3-phosphate dehydrogenase, L-glycerol phosphate dehydrogenase, L-glycerophosphate dehydrogenase, mGPDH, NAD+-dependent glycerol 3-phosphate dehydrogenase, NAD+-dependent glycerol-3-phosphate dehydrogenase, NAD+-GPDH, NAD-alpha-glycerophosphate dehydrogenase, NAD-dependent cG3PDH, NAD-dependent glycerol phosphate dehydrogenase, NAD-dependent glycerol-3-phosphate dehydrogenase, NAD-L-glycerol-3-phosphate dehydrogenase, NAD-linked glycerol 3-phosphate dehydrogenase, NADH-dihydroxyacetone phosphate reductase

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.8 glycerol-3-phosphate dehydrogenase (NAD+)

General Stability

General Stability on EC 1.1.1.8 - glycerol-3-phosphate dehydrogenase (NAD+)

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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
2-mercaptoethanol does not stabilize
-
2-mercaptoethanol stabilizes during purification
20% polyethylene glycol stabilizes during purification
ammonium sulfate inactivates
-
ammonium sulfate stabilizes
bovine serum albumin stabilizes
-
charcoal inactivates rabbit enzyme, restorable by thiamic acid
-
dithiothreitol does not stabilize
-
dithiothreitol stabilizes
EDTA stabilizes
even cold dilute solutions in 0.2 M ammonium sulfate at pH 5.8 are stable for weeks
-
freezing inactivates completely, ammonium sulfate prevents inactivation
-
lyophilization inactivates
-
NAD+ stabilizes
-
NADH stabilizes
-
NADH stabilizes preparations of low ionic strength
-
phenylmethane-sulfonyl fluoride stabilizes during purification
-
phosphate buffer stabilizes
-
redistilled water prevents denaturation in absence of salts
-
repeated freezing and thawing results in rapid loss of activity, to some extent restorable at room temperature
-
substrates stabilize dilute preparation
-
the enzyme displays rapid induction as well as decay upon dissappearance of a hormonal stimulus (thyroid hormone), indicating a rather short half-life of the enzyme
-