Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | at physiological concentration, 10 mM 90% inhibition | Saccharomyces cerevisiae | |
ATP | at physiological concentration, 10 mM 95% inhibition | Saccharomyces cerevisiae | |
fructose-1,6-bisphosphate | at physiological concentration, non-competitive | Saccharomyces cerevisiae | |
additional information | no inhibition by NADPH, acetaldehyde, glycerol, ethanol | Saccharomyces cerevisiae | |
NAD+ | competitive inhibitor to NADH at physiological concentration | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.023 | - |
NADH | - |
Saccharomyces cerevisiae | |
0.54 | - |
dihydroxyacetone phosphate | - |
Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
2 * 42000, SDS-PAGE | Saccharomyces cerevisiae |
68000 | - |
gel filtration | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
sn-glycerol 3-phosphate + NAD+ | Saccharomyces cerevisiae | oxidation at 3% the reaction rate of dihydroxyacetone phosphate reduction at pH 7.0 | dihydroxyacetone phosphate + NADH + H+ | - |
r | |
sn-glycerol 3-phosphate + NAD+ | Saccharomyces cerevisiae H44-3D | oxidation at 3% the reaction rate of dihydroxyacetone phosphate reduction at pH 7.0 | dihydroxyacetone phosphate + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Saccharomyces cerevisiae H44-3D | - |
- |
- |
Purification (Comment) | Organism |
---|---|
affinity chromatography | Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
41.1 | - |
- |
Saccharomyces cerevisiae |
Storage Stability | Organism |
---|---|
-20°C, in 0.1 M Tris-HCl, 2 mM 2-mercaptoethanol, pH 7.0, 50% polyethylene glycol 2000, 5 mM NADH or dihydroxyacetone phosphate, unstable | Saccharomyces cerevisiae |
-80°C, in 0.1 M Tris-HCl, 2 mM 2-mercaptoethanol, pH 7.0, 50% polyethylene glycol 2000, 5 mM NADH or dihydroxyacetone phosphate, unstable | Saccharomyces cerevisiae |
4°C, 1 M Tris-HCl, 2 mM 2-mercaptoethanol, pH 7.0, eluted from Sephadex G-100 column, 90% activity remained after several weeks | Saccharomyces cerevisiae |
4°C, stable for several weeks in 1 M Tris/HCl buffer with 2-mercaptoethanol, pH 7.0 | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | glycerol | Saccharomyces cerevisiae | ? | - |
? | |
additional information | no substrates: dihydroxyacetone | Saccharomyces cerevisiae | ? | - |
? | |
additional information | glycerol | Saccharomyces cerevisiae H44-3D | ? | - |
? | |
additional information | no substrates: dihydroxyacetone | Saccharomyces cerevisiae H44-3D | ? | - |
? | |
sn-glycerol 3-phosphate + NAD+ | - |
Saccharomyces cerevisiae | dihydroxyacetone phosphate + NADH + H+ | - |
r | |
sn-glycerol 3-phosphate + NAD+ | oxidation at 3% the reaction rate of dihydroxyacetone phosphate reduction at pH 7.0 | Saccharomyces cerevisiae | dihydroxyacetone phosphate + NADH + H+ | - |
r | |
sn-glycerol 3-phosphate + NAD+ | - |
Saccharomyces cerevisiae H44-3D | dihydroxyacetone phosphate + NADH + H+ | - |
r | |
sn-glycerol 3-phosphate + NAD+ | oxidation at 3% the reaction rate of dihydroxyacetone phosphate reduction at pH 7.0 | Saccharomyces cerevisiae H44-3D | dihydroxyacetone phosphate + NADH + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 42000, SDS-PAGE | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
maximum rate of dihydroxyacetone phosphate reduction | Saccharomyces cerevisiae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | dihydroxyacetone reduction, activity decreases rapidly below pH 7.0 and above pH 8.0, more rapidly at acidic values | Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | no activity with NADPH | Saccharomyces cerevisiae | |
NAD+ | - |
Saccharomyces cerevisiae | |
NADH | - |
Saccharomyces cerevisiae |