1.1.1.8: glycerol-3-phosphate dehydrogenase (NAD+)

This is an abbreviated version, for detailed information about glycerol-3-phosphate dehydrogenase (NAD+), go to the full flat file.

Reaction

sn-glycerol 3-phosphate
+
acceptor
=
glycerone phosphate
+
reduced acceptor

Synonyms

(NAD+)-dependent G3pdh, (NAD+)-dependent glycerol-3-phosphate dehydrogenase, alpha glycerophosphate dehydrogenase, alpha-glycerol phosphate dehydrogenase (NAD), alpha-glycerophosphate dehydrogenase (NAD), Cagpd1, Cagpd2, cG3PDH, CgGPD, cGPdH, cytoplasmic NAD-dependent glycerol-3-phosphate dehydrogenase, dehydrogenase, glycerol phosphate, DhGPD1, G-3-P dehydrogenase, G3P dehydrogenase, G3PD, G3PDH, GDM, GDP2, GLPD, glycerol 1-phosphate dehydrogenase, glycerol 3-phosphate dehydrogenase, glycerol 3-phosphate dehydrogenase 1, glycerol phosphate dehydrogenase (NAD), glycerol-3-phosphate dehydrogenase, glycerol-3-phosphate dehydrogenase 1, glycerophosphate dehydrogenase (NAD), GPD, GPD1, GPDH, GPDH-1, GPDH-2, GPDH1, GPDH2, GPDH: NAD+ 2 oxidorreductase, GPDHc1, hydroglycerophosphate dehydrogenase, L-alpha-glycerol phosphate dehydrogenase, L-alpha-glycerophosphate dehydrogenase, L-glycerol 3-phosphate dehydrogenase, L-glycerol phosphate dehydrogenase, L-glycerophosphate dehydrogenase, mGPDH, NAD+-dependent glycerol 3-phosphate dehydrogenase, NAD+-dependent glycerol-3-phosphate dehydrogenase, NAD+-GPDH, NAD-alpha-glycerophosphate dehydrogenase, NAD-dependent cG3PDH, NAD-dependent glycerol phosphate dehydrogenase, NAD-dependent glycerol-3-phosphate dehydrogenase, NAD-L-glycerol-3-phosphate dehydrogenase, NAD-linked glycerol 3-phosphate dehydrogenase, NADH-dihydroxyacetone phosphate reductase

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.8 glycerol-3-phosphate dehydrogenase (NAD+)

Purification

Purification on EC 1.1.1.8 - glycerol-3-phosphate dehydrogenase (NAD+)

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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
2 allelic forms of the enzyme; affinity chromatography
-
2 isozymes
2 isozymes with distinct isoelectric points in brain
-
3 allelic forms distinguishable by electrophoresis
-
3 isozymes in adults and 1 in larvae, distinct in isoelectric focusing; affinity chromatography
-
3 isozymes, product of the same gene mapped to left arm of chromosome II, isozyme 1: flight muscle, isozyme 3: larval and adult fat body, isozyme 2: heterodimer of 1 and 3; affinity chromatography
-
4 isozymes in testes and 5 isozymes in liver, distinct in isoelectric focusing
-
6 to 8 isozymes of bumblebee flight muscle enzyme
-
affinity chromatography
dye-affinity chromatography
-
GSTrap column chromatography; GSTrap column chromatography
ion-exchange chromatography combined with affinity elution, 2 isozymes that differ in charge by analytical PAGE
-
native enzyme 50fold and 176fold from liver and muscle, respectively, by anion exchange chromatography, gel filtration, and affinity chromatography
-
preparative isoelectric focusing
-
recombinant enzyme from Escherichia coli strain BL21 by anion exchange chromatography to over 95% purity
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
-
sequential affinity chromatography; several isozymes, distinct in isoelectric points
-
several isozymes, distinct in isoelectric points
-