1.1.1.8: glycerol-3-phosphate dehydrogenase (NAD+)

This is an abbreviated version, for detailed information about glycerol-3-phosphate dehydrogenase (NAD+), go to the full flat file.

Reaction

sn-glycerol 3-phosphate
+
acceptor
=
glycerone phosphate
+
reduced acceptor

Synonyms

(NAD+)-dependent G3pdh, (NAD+)-dependent glycerol-3-phosphate dehydrogenase, alpha glycerophosphate dehydrogenase, alpha-glycerol phosphate dehydrogenase (NAD), alpha-glycerophosphate dehydrogenase (NAD), Cagpd1, Cagpd2, cG3PDH, CgGPD, cGPdH, cytoplasmic NAD-dependent glycerol-3-phosphate dehydrogenase, dehydrogenase, glycerol phosphate, DhGPD1, G-3-P dehydrogenase, G3P dehydrogenase, G3PD, G3PDH, GDM, GDP2, GLPD, glycerol 1-phosphate dehydrogenase, glycerol 3-phosphate dehydrogenase, glycerol 3-phosphate dehydrogenase 1, glycerol phosphate dehydrogenase (NAD), glycerol-3-phosphate dehydrogenase, glycerol-3-phosphate dehydrogenase 1, glycerophosphate dehydrogenase (NAD), GPD, GPD1, GPDH, GPDH-1, GPDH-2, GPDH1, GPDH2, GPDH: NAD+ 2 oxidorreductase, GPDHc1, hydroglycerophosphate dehydrogenase, L-alpha-glycerol phosphate dehydrogenase, L-alpha-glycerophosphate dehydrogenase, L-glycerol 3-phosphate dehydrogenase, L-glycerol phosphate dehydrogenase, L-glycerophosphate dehydrogenase, mGPDH, NAD+-dependent glycerol 3-phosphate dehydrogenase, NAD+-dependent glycerol-3-phosphate dehydrogenase, NAD+-GPDH, NAD-alpha-glycerophosphate dehydrogenase, NAD-dependent cG3PDH, NAD-dependent glycerol phosphate dehydrogenase, NAD-dependent glycerol-3-phosphate dehydrogenase, NAD-L-glycerol-3-phosphate dehydrogenase, NAD-linked glycerol 3-phosphate dehydrogenase, NADH-dihydroxyacetone phosphate reductase

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.8 glycerol-3-phosphate dehydrogenase (NAD+)

Activating Compound

Activating Compound on EC 1.1.1.8 - glycerol-3-phosphate dehydrogenase (NAD+)

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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
ATP
-
slight activation
bovine serum albumin
-
activity increased
-
Dihydrolipoic acid
-
stimulation of chloroplastic form, 147% of enzyme activity
dipotassium malate
-
slight activation at low ionic strength
dithiothreitol
-
stimulation of chloroplastic form, 150% of enzyme activity
EDTA
-
without EDTA the rates are about 60% of the maximal rate
fructose 2,6-bisphosphate
-
0.025 mM maximum stimulation of 2.3fold of cytosolic form, not chloroplastic form, no stimulation by fructose-1,6-bisphosphate
glycerol
-
competitive activator with respect to dihydroxyacetone phosphate above 30 mM
Glycine buffer
-
0.9 M, slight activation
-
phosphate
-
around 5 mM, activation, chloroplastic form
phosphite dianion
-
allosteric activation. Separate binding of the second substrate piece phosphite dianion strongly activates GPDH for catalysis of the reduction of glycolaldehyde by NADH
phosphogluconate
-
0.005 mM slightly stimulates chloroplastic form
potassium glutamate
-
up to 0.1 M, activation, high activity also at high ionic strength
additional information
-