1.1.1.8: glycerol-3-phosphate dehydrogenase (NAD+)

This is an abbreviated version, for detailed information about glycerol-3-phosphate dehydrogenase (NAD+), go to the full flat file.

Reaction

sn-glycerol 3-phosphate
+
acceptor
=
glycerone phosphate
+
reduced acceptor

Synonyms

(NAD+)-dependent G3pdh, (NAD+)-dependent glycerol-3-phosphate dehydrogenase, alpha glycerophosphate dehydrogenase, alpha-glycerol phosphate dehydrogenase (NAD), alpha-glycerophosphate dehydrogenase (NAD), Cagpd1, Cagpd2, cG3PDH, CgGPD, cGPdH, cytoplasmic NAD-dependent glycerol-3-phosphate dehydrogenase, dehydrogenase, glycerol phosphate, DhGPD1, G-3-P dehydrogenase, G3P dehydrogenase, G3PD, G3PDH, GDM, GDP2, GLPD, glycerol 1-phosphate dehydrogenase, glycerol 3-phosphate dehydrogenase, glycerol 3-phosphate dehydrogenase 1, glycerol phosphate dehydrogenase (NAD), glycerol-3-phosphate dehydrogenase, glycerol-3-phosphate dehydrogenase 1, glycerophosphate dehydrogenase (NAD), GPD, GPD1, GPDH, GPDH-1, GPDH-2, GPDH1, GPDH2, GPDH: NAD+ 2 oxidorreductase, GPDHc1, hydroglycerophosphate dehydrogenase, L-alpha-glycerol phosphate dehydrogenase, L-alpha-glycerophosphate dehydrogenase, L-glycerol 3-phosphate dehydrogenase, L-glycerol phosphate dehydrogenase, L-glycerophosphate dehydrogenase, mGPDH, NAD+-dependent glycerol 3-phosphate dehydrogenase, NAD+-dependent glycerol-3-phosphate dehydrogenase, NAD+-GPDH, NAD-alpha-glycerophosphate dehydrogenase, NAD-dependent cG3PDH, NAD-dependent glycerol phosphate dehydrogenase, NAD-dependent glycerol-3-phosphate dehydrogenase, NAD-L-glycerol-3-phosphate dehydrogenase, NAD-linked glycerol 3-phosphate dehydrogenase, NADH-dihydroxyacetone phosphate reductase

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.8 glycerol-3-phosphate dehydrogenase (NAD+)

Cloned

Cloned on EC 1.1.1.8 - glycerol-3-phosphate dehydrogenase (NAD+)

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence determination and analysis, expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3)
-
expressed in a gpd1DELTAgpd2DELTA double deletion mutant of Saccharomyces cerevisiae; expressed in a gpd1DELTAgpd2DELTA double deletion mutant of Saccharomyces cerevisiae
expressed in Escherichia coli BL21(DE3) pLysScells; expressed in Escherichia coli BL21(DE3) pLysScells; expressed in Escherichia coli BL21(DE3) pLysS cells; expressed in Escherichia coli BL21(DE3) pLysS cells
expressed in Escherichia coli with the maltose-binding protein fusion system
-
expression in CHO cells
-
expression in Escherichia coli BL-21 DE3 as a fusion protein with glutathione S-transferase
expression in Saccharomyces cerevisiae
-
expression in Saccharomyces cerevisiae. Although the gene is functional in Saccharomyces cerevisiae, its heterologous expression is not efficient, suggesting that the regulatory mechanism may not be shared by Debaryomyces hansenii and Saccharomyces cerevisiae
-
gene YDL022W, phylogenetic analysis, expression in Escherichia coli strain BL21
genes CrGPDH1, CrGPDH2, and CrGPDH3, DNA and amino acid sequence determination and analysis, phylogenetic analysis
-
GPD1, phylogenetic analysis, relative quantitative real-time PCR, expression of GFP-tagged GPD1 in PK-15 cells
recombinant in Escherichia coli