1.1.1.8: glycerol-3-phosphate dehydrogenase (NAD+)

This is an abbreviated version, for detailed information about glycerol-3-phosphate dehydrogenase (NAD+), go to the full flat file.

Reaction

sn-glycerol 3-phosphate
+
acceptor
=
glycerone phosphate
+
reduced acceptor

Synonyms

(NAD+)-dependent G3pdh, (NAD+)-dependent glycerol-3-phosphate dehydrogenase, alpha glycerophosphate dehydrogenase, alpha-glycerol phosphate dehydrogenase (NAD), alpha-glycerophosphate dehydrogenase (NAD), Cagpd1, Cagpd1p, Cagpd2, Cagpd2p, cG3PDH, CgGPD, cGPdH, cytoplasmic NAD-dependent glycerol-3-phosphate dehydrogenase, cytosolic GPDH, dehydrogenase, glycerol phosphate, DhGPD1, G-3-P dehydrogenase, G3P dehydrogenase, G3PD, G3PD1, G3PD2, G3PD3, G3PDH, GDM, GDP2, GLPD, GLY1, glycerol 1-phosphate dehydrogenase, glycerol 3-phosphate dehydrogenase, glycerol 3-phosphate dehydrogenase 1, glycerol phosphate dehydrogenase (NAD), glycerol-3-phosphate dehydrogenase, glycerol-3-phosphate dehydrogenase 1, glycerophosphate dehydrogenase (NAD), GPD, GPD1, Gpd1p, gpd2p, GPDH, GPDH-1, GPDH-2, GPDH1, GPDH2, GPDH3, GPDH: NAD+ 2 oxidoreductase, GPDH: NAD+ 2 oxidorreductase, GPDHc1, hydroglycerophosphate dehydrogenase, L-alpha-glycerol phosphate dehydrogenase, L-alpha-glycerophosphate dehydrogenase, L-glycerol 3-phosphate dehydrogenase, L-glycerol phosphate dehydrogenase, L-glycerophosphate dehydrogenase, mGPDH, NAD+-dependent G3P dehydrogenase, NAD+-dependent glycerol 3-phosphate dehydrogenase, NAD+-dependent glycerol-3-phosphate dehydrogenase, NAD+-GPDH, NAD-alpha-glycerophosphate dehydrogenase, NAD-dependent cG3PDH, NAD-dependent glycerol phosphate dehydrogenase, NAD-dependent glycerol-3-phosphate dehydrogenase, NAD-L-glycerol-3-phosphate dehydrogenase, NAD-linked glycerol 3-phosphate dehydrogenase, NADH-dihydroxyacetone phosphate reductase, NADP+-dependent glycerol 3-phosphate dehydrogenase, SFD1, SFD1/GLY1

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.8 glycerol-3-phosphate dehydrogenase (NAD+)

Cloned

Cloned on EC 1.1.1.8 - glycerol-3-phosphate dehydrogenase (NAD+)

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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence determination and analysis, expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3)
-
expressed in a gpd1DELTAgpd2DELTA double deletion mutant of Saccharomyces cerevisiae; expressed in a gpd1DELTAgpd2DELTA double deletion mutant of Saccharomyces cerevisiae; expressed in Saccharomyces cerevisiae strain BY4741; expressed in Saccharomyces cerevisiae strain BY4741; gene Cagpd1, DNA and amino acid sequence determination and analysis, phylogenetic analysis, the intronless genes Cagpd1 and Cagpd2, encoding the two isozymes of the organism, are both predicted to encode a 378 amino acid polypeptide, and the deduced amino acid sequences mutually show 76% identity. Genes Cagpd1 and Cagpd2 are located tandemly in a locus of genomic DNA within a 262 bp interval. Recombinant expression in the Sacchhyromyces cerevisiae strain BY4741 gpd1DELTAgpd2DELTA double mutant; gene Cagpd2, DNA and amino acid sequence determination and analysis, phylogenetic analysis, the intronless genes Cagpd1 and Cagpd2, encoding the two isozymes of the organism, are both predicted to encode a 378 amino acid polypeptide, and the deduced amino acid sequences mutually show 76% identity. Genes Cagpd1 and Cagpd2 are located tandemly in a locus of genomic DNA within a 262 bp interval. Recombinant expression in the Sacchhyromyces cerevisiae strain BY4741 gpd1DELTAgpd2DELTA double mutant
A0A140JW76, A0A140JW77, A0A14OJW76, A0A14OJW77
expressed in Escherichia coli BL21(DE3) cells; gene GPDH1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
expressed in Escherichia coli BL21(DE3) pLysScells; expressed in Escherichia coli BL21(DE3) pLysScells; expressed in Escherichia coli BL21(DE3) pLysS cells; expressed in Escherichia coli BL21(DE3) pLysS cells
expressed in Escherichia coli with the maltose-binding protein fusion system
-
expressed in Oryza sativa; gene SFD1, overexpression of the enzyme under the control of the maize ubiquitin promoter in Oryza sativa Japonica rice cultivar TP309 chloroplasts by transformation of embryogenic calli using Agrobacterium tumefaciens strain LBA4404, SFD1-GFP localization in rice leaf sheath cells
expressed in Saccharomyces cerevisiae strain w303-1a; expressed in Saccharomyces cerevisiae strain w303-1a
expression in CHO cells
-
expression in Escherichia coli BL-21 DE3 as a fusion protein with glutathione S-transferase
-
expression in Saccharomyces cerevisiae
-
expression in Saccharomyces cerevisiae. Although the gene is functional in Saccharomyces cerevisiae, its heterologous expression is not efficient, suggesting that the regulatory mechanism may not be shared by Debaryomyces hansenii and Saccharomyces cerevisiae
-
gene GPD1, quantitative real-time PCR expression analysis
gene YDL022W, phylogenetic analysis, expression in Escherichia coli strain BL21
genes CrGPDH1, CrGPDH2, and CrGPDH3, DNA and amino acid sequence determination and analysis, phylogenetic analysis
-
GPD1, phylogenetic analysis, relative quantitative real-time PCR, expression of GFP-tagged GPD1 in PK-15 cells
recombinant in Escherichia coli