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1.1.1.6: glycerol dehydrogenase

This is an abbreviated version!
For detailed information about glycerol dehydrogenase, go to the full flat file.

Word Map on EC 1.1.1.6

Reaction

glycerol
+
NAD+
=
Glycerone
+
NADH
+
H+

Synonyms

B4100_2156, CglD, dehydrogenase, glycerol, GDH, GDH2, GLD, Gld3, GldA, GLDH , GlhA, glycerin dehydrogenase, glycerol dehydrogenase, glycerol NAD 2-oxidoreductase, glycerol:NAD+ 2-oxidoreductase, GlyDH, Gro dehydrogenase, GroDHase, GSH, gycerol dehydrogenase, NAD+-dependent glycerol dehydrogenase, NAD-linked glycerol dehydrogenase, NAD-specific glycerol dehydrogenase, TmGlyDH, TTHADH

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.6 glycerol dehydrogenase

Purification

Purification on EC 1.1.1.6 - glycerol dehydrogenase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene gldA, recombinant C-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, ultrafiltration, and dialysis
GST-tagged protein
-
HisTrap column chromatography and Superdex 200 gel filtration
immobilized metal ion affinity chromatography (Co2+)
immobilized metal ion affinity chromatography (Ni2+)
purified by Ni-affinity chromatography
-
purified with 25fold purification and 68% yield using a single chromatographic step
-
recombinant His-tagged chimeric enzyme GDH-NOX from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant His-tagged enzyme from Escherichia coli strain BL21 (DE3) by immobilized metal affinity chromatography or by heat shock treatment, both resulting in about the same protein purity of over 90%
-
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
-
recombinant His-tagged enzyme from strain BL21(DE3) by nickel affinity chromatography
-
recombinant mutant enzyme S305C
-
recombinant N-terminally His6-tagged enzyme from Escherichia coli train BL21(DE3)pLysS by nicke affinity chromatography and gel filtration
-
recombinant protein
recombinant protein, purified to homogeneity using a combination of heat-shock and chromatographic methods
recombinent His-tagged enzyme from Escherichia coli strain BL21(DE3) pET-32a by nickel affinity chromatography, desalting gel filtration, and ultrafiltration