1.1.1.6: glycerol dehydrogenase

This is an abbreviated version, for detailed information about glycerol dehydrogenase, go to the full flat file.

Reaction

glycerol
+
NAD+
=
Glycerone
+
NADH
+
H+

Synonyms

CglD, dehydrogenase, glycerol, GDH, GDH2, GLD, GldA, GLDH , GlhA, glycerin dehydrogenase, glycerol dehydrogenase, glycerol NAD 2-oxidoreductase, glycerol:NAD+ 2-oxidoreductase, Gro dehydrogenase, GroDHase, gycerol dehydrogenase, NAD-linked glycerol dehydrogenase, NAD-specific glycerol dehydrogenase

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.6 glycerol dehydrogenase

Purification

Purification on EC 1.1.1.6 - glycerol dehydrogenase

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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
GST-tagged protein
-
immobilized metal ion affinity chromatography (Co2+)
immobilized metal ion affinity chromatography (Ni2+); purity estimated from SDS-PAGE: 95%
purified by Ni-affinity chromatography; recombinant His-tagged enzyme from strain BL21(DE3) by nickel affinity chromatography
-
recombinant His-tagged enzyme from Escherichia coli strain BL21 (DE3) by immobilized metal affinity chromatography or by heat shock treatment, both resulting in about the same protein purity of over 90%
-
recombinant mutant enzyme S305C
-