Cloned (Comment) | Organism |
---|---|
His-tagged version expressed in Escherichia coli BL21(DE3) | Escherichia coli |
His-tagged version expressed in Escherichia coli BL21(DE3) | Klebsiella pneumoniae |
His-tagged version expressed in Escherichia coli BL21(DE3) | Salmonella enterica |
Protein Variants | Comment | Organism |
---|---|---|
A15T/D16G/V17A/N19K/E23D/Q45E/S46E/T47M/V48L/E49R/F52L/K53A/V58A/V59A/Q70H/D74N/G78D/E81G/T82N/Q83K/G86T/I88V/G108N/R139S/L142M/N145R/K155Q/V256I/L260M | mutant selected from a DNA shuffling library (Escherichia coli, Salmonella enterica, Klebsiella pneumoniae) | Escherichia coli |
D121A | D121 can potentially hinder the proper binding of substrate 1,3-butanediol due to steric hindrance | Escherichia coli |
D16N/N19A/E23D/L28M/E30N/R31N/Q45E/S46E/V48L/E49R/F52L/K53T/D54G/V58S/G78V/I79V/T82K/A83S/I88V/G108N/R139S/L142M/N145R/K155Q/L211I/G248S/V256I/H268Y/D317E/P319L | mutant selected from a DNA shuffling library (Escherichia coli, Salmonella enterica, Klebsiella pneumoniae) | Escherichia coli |
Q70H/D121A/G193C/E291Q/A310T | mutant selected from a DNA shuffling library (Escherichia coli, Salmonella enterica, Klebsiella pneumoniae) and site-directed mutation D121A | Escherichia coli |
Q70H/D74N/G78D/E81G/T82N/Q83K/C84Y/G86T/I88V/G108N/E134A/E204K/L211I/E215K/I234V/V256I/L260M/E291D/S300C/A302S/E316G/V318I/A320T/I324L/T344D/P345S | mutant selected from a DNA shuffling library (Escherichia coli, Salmonella enterica, Klebsiella pneumoniae) | Escherichia coli |
Q70H/G193C/E291Q/A310T | mutant selected from a DNA shuffling library (Escherichia coli, Salmonella enterica, Klebsiella pneumoniae) | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0165 | - |
NAD+ | wild type protein, pH 9.5, 30°C | Escherichia coli | |
0.0182 | - |
NAD+ | mutant protein Q70H/G193C/E291Q/A310T, pH 9.5, 30°C | Escherichia coli | |
0.4614 | - |
NAD+ | mutant protein Q70H/D121A/G193C/E291Q/A310T, pH 9.5, 30°C | Escherichia coli | |
0.5946 | - |
NAD+ | mutant protein D121A, pH 9.5, 30°C | Escherichia coli | |
11.9 | - |
1,3-butanediol | mutant protein Q70H/G193C/E291Q/A310T, pH 9.5, 30°C | Escherichia coli | |
16.2 | - |
1,3-butanediol | wild type protein, pH 9.5, 30°C | Escherichia coli | |
20.9 | - |
1,3-butanediol | pH 9.5, 30°C | Klebsiella pneumoniae | |
29.2 | - |
1,3-butanediol | pH 9.5, 30°C | Salmonella enterica | |
49.9 | - |
1,3-butanediol | mutant protein D121A, pH 9.5, 30°C | Escherichia coli | |
77.8 | - |
1,3-butanediol | mutant protein Q70H/D121A/G193C/E291Q/A310T, pH 9.5, 30°C | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | - |
Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39000 | - |
x * 39000, SDS-PAGE | Escherichia coli |
39000 | - |
x * 39000, SDS-PAGE | Klebsiella pneumoniae |
39000 | - |
x * 39000, SDS-PAGE | Salmonella enterica |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Klebsiella pneumoniae | - |
- |
- |
Klebsiella pneumoniae MGH78578 | - |
- |
- |
Salmonella enterica | - |
- |
- |
Salmonella enterica ATCC 9150 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
immobilized metal ion affinity chromatography (Co2+) | Escherichia coli |
immobilized metal ion affinity chromatography (Co2+) | Klebsiella pneumoniae |
immobilized metal ion affinity chromatography (Co2+) | Salmonella enterica |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.7 | - |
pH 9.5, 30°C | Klebsiella pneumoniae |
3.9 | - |
pH 9.5, 30°C | Salmonella enterica |
4.8 | - |
wild type protein, pH 9.5, 30°C | Escherichia coli |
6 | - |
mutant protein D16N/N19A/E23D/L28M/E30N/R31N/Q45E/S46E/V48L/E49R/F52L/K53T/D54G/V58S/G78V/I79V/T82K/A83S/I88V/G108N/R139S/L142M/N145R/K155Q/L211I/G248S/V256I/H268Y/D317E/P319L, pH 9.5, 30°C | Escherichia coli |
6.8 | - |
mutant protein A15T/D16G/V17A/N19K/E23D/Q45E/S46E/T47M/V48L/E49R/F52L/K53A/V58A/V59A/Q70H/D74N/G78D/E81G/T82N/Q83K/G86T/I88V/G108N/R139S/L142M/N145R/K155Q/V256I/L260M, pH 9.5, 30°C | Escherichia coli |
7.4 | - |
mutant protein Q70H/D74N/G78D/E81G/T82N/Q83K/C84Y/G86T/I88V/G108N/E134A/E204K/L211I/E215K/I234V/V256I/L260M/E291D/S300C/A302S/E316G/V318I/A320T/I324L/T344D/P345S, pH 9.5, 30°C | Escherichia coli |
13.2 | - |
mutant protein Q70H/G193C/E291Q/A310T, pH 9.5, 30°C | Escherichia coli |
90.7 | - |
mutant protein D121A, pH 9.5, 30°C | Escherichia coli |
126.6 | - |
mutant protein Q70H/D121A/G193C/E291Q/A310T, pH 9.5, 30°C | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,3-butanediol + NAD+ | - |
Escherichia coli | 4-hydroxy-2-butanone + NADH + H+ | - |
? | |
1,3-butanediol + NAD+ | - |
Klebsiella pneumoniae | 4-hydroxy-2-butanone + NADH + H+ | - |
? | |
1,3-butanediol + NAD+ | - |
Salmonella enterica | 4-hydroxy-2-butanone + NADH + H+ | - |
? | |
1,3-butanediol + NAD+ | - |
Salmonella enterica ATCC 9150 | 4-hydroxy-2-butanone + NADH + H+ | - |
? | |
1,3-butanediol + NAD+ | - |
Klebsiella pneumoniae MGH78578 | 4-hydroxy-2-butanone + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 39000, SDS-PAGE | Escherichia coli |
? | x * 39000, SDS-PAGE | Klebsiella pneumoniae |
? | x * 39000, SDS-PAGE | Salmonella enterica |
Synonyms | Comment | Organism |
---|---|---|
glycerol dehydrogenase | - |
Escherichia coli |
glycerol dehydrogenase | - |
Klebsiella pneumoniae |
glycerol dehydrogenase | - |
Salmonella enterica |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.6 | - |
1,3-butanediol | pH 9.5, 30°C | Klebsiella pneumoniae | |
2.7 | - |
1,3-butanediol | pH 9.5, 30°C | Salmonella enterica | |
3.3 | - |
1,3-butanediol | wild type protein, pH 9.5, 30°C | Escherichia coli | |
9.1 | - |
1,3-butanediol | mutant protein Q70H/G193C/E291Q/A310T, pH 9.5, 30°C | Escherichia coli | |
62.4 | - |
1,3-butanediol | mutant protein D121A, pH 9.5, 30°C | Escherichia coli | |
87.2 | - |
1,3-butanediol | mutant protein Q70H/D121A/G193C/E291Q/A310T, pH 9.5, 30°C | Escherichia coli |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.09 | - |
1,3-butanediol | pH 9.5, 30°C | Salmonella enterica | |
0.12 | - |
1,3-butanediol | pH 9.5, 30°C | Klebsiella pneumoniae | |
0.2 | - |
1,3-butanediol | wild type protein, pH 9.5, 30°C | Escherichia coli | |
0.77 | - |
1,3-butanediol | mutant protein Q70H/G193C/E291Q/A310T, pH 9.5, 30°C | Escherichia coli | |
1.12 | - |
1,3-butanediol | mutant protein Q70H/D121A/G193C/E291Q/A310T, pH 9.5, 30°C | Escherichia coli | |
1.26 | - |
1,3-butanediol | mutant protein D121A, pH 9.5, 30°C | Escherichia coli | |
105 | - |
NAD+ | mutant protein D121A, pH 9.5, 30°C | Escherichia coli | |
188 | - |
NAD+ | mutant protein Q70H/D121A/G193C/E291Q/A310T, pH 9.5, 30°C | Escherichia coli | |
200 | - |
NAD+ | wild type protein, pH 9.5, 30°C | Escherichia coli | |
500 | - |
NAD+ | mutant protein Q70H/G193C/E291Q/A310T, pH 9.5, 30°C | Escherichia coli |