1.1.1.6: glycerol dehydrogenase

This is an abbreviated version, for detailed information about glycerol dehydrogenase, go to the full flat file.

Reaction

glycerol
+
NAD+
=
Glycerone
+
NADH
+
H+

Synonyms

CglD, dehydrogenase, glycerol, GDH, GDH2, GLD, GldA, GLDH , GlhA, glycerin dehydrogenase, glycerol dehydrogenase, glycerol NAD 2-oxidoreductase, glycerol:NAD+ 2-oxidoreductase, Gro dehydrogenase, GroDHase, gycerol dehydrogenase, NAD-linked glycerol dehydrogenase, NAD-specific glycerol dehydrogenase

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.6 glycerol dehydrogenase

Specific Activity

Specific Activity on EC 1.1.1.6 - glycerol dehydrogenase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.01
wild type, crude cell extract, pH not specified in the publication, temperature not specified in the publication
0.033
-
anaerobic growth conditions, cells permeabilized with chloroform
0.049
overexpressing cells, crude cell extract, pH not specified in the publication, temperature not specified in the publication
1.05
-
cell free extract
1.26
-
; pH 8.2, 25C
3.7
-
pH 9.5, 30C
3.9
-
pH 9.5, 30C
4.7
cell free extract, wild type; wild type, crude extract, pH not specified in the publication, temperature not specified in the publication
4.8
-
wild type protein, pH 9.5, 30C
6
-
mutant protein D16N/N19A/E23D/L28M/E30N/R31N/Q45E/S46E/V48L/E49R/F52L/K53T/D54G/V58S/G78V/I79V/T82K/A83S/I88V/G108N/R139S/L142M/N145R/K155Q/L211I/G248S/V256I/H268Y/D317E/P319L, pH 9.5, 30C
6.8
-
mutant protein A15T/D16G/V17A/N19K/E23D/Q45E/S46E/T47M/V48L/E49R/F52L/K53A/V58A/V59A/Q70H/D74N/G78D/E81G/T82N/Q83K/G86T/I88V/G108N/R139S/L142M/N145R/K155Q/V256I/L260M, pH 9.5, 30C
7.4
-
mutant protein Q70H/D74N/G78D/E81G/T82N/Q83K/C84Y/G86T/I88V/G108N/E134A/E204K/L211I/E215K/I234V/V256I/L260M/E291D/S300C/A302S/E316G/V318I/A320T/I324L/T344D/P345S, pH 9.5, 30C
13.2
-
mutant protein Q70H/G193C/E291Q/A310T, pH 9.5, 30C
90.7
-
mutant protein D121A, pH 9.5, 30C
107
-
commercial preparation
126.6
-
mutant protein Q70H/D121A/G193C/E291Q/A310T, pH 9.5, 30C
220
cell free extract, recombinant strain (over-expression of gycerol dehydrogenase); recombinant strain (overexpression), crude extract, pH not specified in the publication, temperature not specified in the publication
additional information
-
assay development for NADH production by recombinant enzyme in reverse micelles, micelle size optimization, overview