BRENDA - Enzyme Database show
show all sequences of 1.1.1.6

Production and characterization of Escherichia coli glycerol dehydrogenase as a tool for glycerol recycling

Piattoni, C.; Figueroa, C.; Asencion Diez, M.; Parcerisa, I.; Antuna, S.; Comelli, R.; Guerrero, S.; Beccaria, A.; Iglesias, A.; Process Biochem. 48, 406-412 (2013)
No PubMed abstract available

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene gldA from Escherichia coli strain JM109, expression of His-tagged enzyme in Escherichia coli strain BL21 (DE3), subcloning in Escherichia coli TOP10 cells
Escherichia coli
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
kinetic study with different effectors, overview
Escherichia coli
0.05
-
NADH
pH 6.5, 30°C, recombinant enzyme
Escherichia coli
0.07
-
NADH
pH 6.5, 30°C, recombinant enzyme, in presence of 20 mM KCl
Escherichia coli
0.08
-
NADH
pH 6.5, 30°C, recombinant enzyme, in presence of 30 mM NH4Cl
Escherichia coli
0.15
-
Glycerone
pH 6.5, 30°C, recombinant enzyme
Escherichia coli
0.22
-
Glycerone
pH 6.5, 30°C, recombinant enzyme, in presence of 30 mM NH4Cl
Escherichia coli
0.24
-
Glycerone
pH 6.5, 30°C, recombinant enzyme, in presence of 20 mM KCl
Escherichia coli
0.81
-
NAD+
pH 10.3, 30°C, recombinant enzyme
Escherichia coli
1
-
NAD+
pH 10.3, 30°C, recombinant enzyme, in presence of 30 mM NH4Cl
Escherichia coli
3.2
-
NAD+
pH 10.3, 30°C, recombinant enzyme, in presence of 20 mM KCl
Escherichia coli
47
-
glycerol
pH 10.3, 30°C, recombinant enzyme, in presence of 20 mM KCl
Escherichia coli
76
-
glycerol
pH 10.3, 30°C, recombinant enzyme
Escherichia coli
81
-
glycerol
pH 10.3, 30°C, recombinant enzyme, in presence of 30 mM NH4Cl
Escherichia coli
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
K+
activates, less effective than NH4+
Escherichia coli
NH4+
activates, 4fold increase in Vmax produced by NH4Cl in the direction of glycerol oxidation, and 70fold reduction in the Km for dihydroxyaceton and 2fold increase in the Vmax with 30 mM NH4Cl added to the dihydroxyaceton reduction reaction
Escherichia coli
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
39800
-
8 * 40000, recombinant His-tagged enzyme, SDS-PAGE, 8 * 39800, about, sequence calculation
Escherichia coli
40000
-
8 * 40000, recombinant His-tagged enzyme, SDS-PAGE, 8 * 39800, about, sequence calculation
Escherichia coli
320000
-
recombinant His-tagged enzyme, gel filtration
Escherichia coli
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
glycerol + NAD+
Escherichia coli
-
glycerone + NADH + H+
-
-
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
-
gene gldA
-
Purification (Commentary)
Commentary
Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21 (DE3) by immobilized metal affinity chromatography or by heat shock treatment, both resulting in about the same protein purity of over 90%
Escherichia coli
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
glycerol + NAD+
-
723679
Escherichia coli
glycerone + NADH + H+
-
-
-
r
additional information
ability of the enzyme to use glycerol from biodiesel waste as substrate, overview
723679
Escherichia coli
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
octamer
8 * 40000, recombinant His-tagged enzyme, SDS-PAGE, 8 * 39800, about, sequence calculation
Escherichia coli
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
60
-
-
Escherichia coli
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
20
80
activity range, profile overview
Escherichia coli
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
dihydroxyaceton reduction reaction
Escherichia coli
11
-
glycerol oxidation reaction
Escherichia coli
pH Range
pH Minimum
pH Maximum
Commentary
Organism
3
9
dihydroxyaceton reduction reaction, activity range, profile overview
Escherichia coli
7.5
12
glycerol oxidation reaction, activity range, profile overview
Escherichia coli
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
-
Escherichia coli
NADH
-
Escherichia coli
Cloned(Commentary) (protein specific)
Commentary
Organism
gene gldA from Escherichia coli strain JM109, expression of His-tagged enzyme in Escherichia coli strain BL21 (DE3), subcloning in Escherichia coli TOP10 cells
Escherichia coli
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Escherichia coli
NADH
-
Escherichia coli
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
kinetic study with different effectors, overview
Escherichia coli
0.05
-
NADH
pH 6.5, 30°C, recombinant enzyme
Escherichia coli
0.07
-
NADH
pH 6.5, 30°C, recombinant enzyme, in presence of 20 mM KCl
Escherichia coli
0.08
-
NADH
pH 6.5, 30°C, recombinant enzyme, in presence of 30 mM NH4Cl
Escherichia coli
0.15
-
Glycerone
pH 6.5, 30°C, recombinant enzyme
Escherichia coli
0.22
-
Glycerone
pH 6.5, 30°C, recombinant enzyme, in presence of 30 mM NH4Cl
Escherichia coli
0.24
-
Glycerone
pH 6.5, 30°C, recombinant enzyme, in presence of 20 mM KCl
Escherichia coli
0.81
-
NAD+
pH 10.3, 30°C, recombinant enzyme
Escherichia coli
1
-
NAD+
pH 10.3, 30°C, recombinant enzyme, in presence of 30 mM NH4Cl
Escherichia coli
3.2
-
NAD+
pH 10.3, 30°C, recombinant enzyme, in presence of 20 mM KCl
Escherichia coli
47
-
glycerol
pH 10.3, 30°C, recombinant enzyme, in presence of 20 mM KCl
Escherichia coli
76
-
glycerol
pH 10.3, 30°C, recombinant enzyme
Escherichia coli
81
-
glycerol
pH 10.3, 30°C, recombinant enzyme, in presence of 30 mM NH4Cl
Escherichia coli
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
K+
activates, less effective than NH4+
Escherichia coli
NH4+
activates, 4fold increase in Vmax produced by NH4Cl in the direction of glycerol oxidation, and 70fold reduction in the Km for dihydroxyaceton and 2fold increase in the Vmax with 30 mM NH4Cl added to the dihydroxyaceton reduction reaction
Escherichia coli
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
39800
-
8 * 40000, recombinant His-tagged enzyme, SDS-PAGE, 8 * 39800, about, sequence calculation
Escherichia coli
40000
-
8 * 40000, recombinant His-tagged enzyme, SDS-PAGE, 8 * 39800, about, sequence calculation
Escherichia coli
320000
-
recombinant His-tagged enzyme, gel filtration
Escherichia coli
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
glycerol + NAD+
Escherichia coli
-
glycerone + NADH + H+
-
-
r
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21 (DE3) by immobilized metal affinity chromatography or by heat shock treatment, both resulting in about the same protein purity of over 90%
Escherichia coli
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
glycerol + NAD+
-
723679
Escherichia coli
glycerone + NADH + H+
-
-
-
r
additional information
ability of the enzyme to use glycerol from biodiesel waste as substrate, overview
723679
Escherichia coli
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
octamer
8 * 40000, recombinant His-tagged enzyme, SDS-PAGE, 8 * 39800, about, sequence calculation
Escherichia coli
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
60
-
-
Escherichia coli
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
20
80
activity range, profile overview
Escherichia coli
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
dihydroxyaceton reduction reaction
Escherichia coli
11
-
glycerol oxidation reaction
Escherichia coli
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
3
9
dihydroxyaceton reduction reaction, activity range, profile overview
Escherichia coli
7.5
12
glycerol oxidation reaction, activity range, profile overview
Escherichia coli
General Information
General Information
Commentary
Organism
evolution
the enzyme belongs to the medium-chain dehydrogenase/reductase, MDRase, superfamily
Escherichia coli
metabolism
in Escherichia coli, the enzyme catalyzes the first step in fermentative glycerol metabolism to produce dihydroxyacetone, biochemical transformation pathway of glycerol, overview
Escherichia coli
physiological function
GroDHase is mainly involved in Gro utilization as a carbon and energy source
Escherichia coli
General Information (protein specific)
General Information
Commentary
Organism
evolution
the enzyme belongs to the medium-chain dehydrogenase/reductase, MDRase, superfamily
Escherichia coli
metabolism
in Escherichia coli, the enzyme catalyzes the first step in fermentative glycerol metabolism to produce dihydroxyacetone, biochemical transformation pathway of glycerol, overview
Escherichia coli
physiological function
GroDHase is mainly involved in Gro utilization as a carbon and energy source
Escherichia coli
Other publictions for EC 1.1.1.6
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
723679
Piattoni
-
Production and characterizatio ...
Escherichia coli
Process Biochem.
48
406-412
2013
-
-
1
-
-
-
-
13
-
2
3
1
-
1
-
-
1
-
-
-
-
-
2
1
1
1
-
-
2
2
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
13
-
2
3
1
-
-
-
1
-
-
-
-
2
1
1
1
-
-
2
2
-
-
-
3
3
-
-
-
710902
Camacho Morales
Analysis of glycerol dehydroge ...
Mucor circinelloides, Mucor circinelloides YR-1
Antonie van Leeuwenhoek
98
437-445
2010
-
-
-
-
-
-
-
-
-
-
-
-
-
7
-
-
-
-
-
-
-
-
4
-
-
-
-
-
1
-
-
-
-
-
-
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-
-
-
-
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-
-
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-
-
-
-
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-
-
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-
-
-
-
4
-
-
-
-
-
1
-
-
-
1
-
-
1
-
-
710976
Matsuzawa
The gld1+ gene encoding glycer ...
Schizosaccharomyces pombe, Schizosaccharomyces pombe ARC039
Appl. Microbiol. Biotechnol.
87
715-727
2010
-
-
1
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
2
-
2
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-
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1
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-
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-
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
710980
Zhang
Engineering of glycerol dehydr ...
Escherichia coli, Klebsiella pneumoniae, Klebsiella pneumoniae MGH78578, Salmonella enterica, Salmonella enterica ATCC 9150
Appl. Microbiol. Biotechnol.
88
117-124
2010
-
-
3
-
6
-
-
10
-
1
3
-
-
9
-
-
3
-
-
-
9
-
5
3
-
-
-
6
-
-
-
-
-
-
-
-
-
3
-
-
6
-
-
-
-
10
-
1
3
-
-
-
-
3
-
-
9
-
5
3
-
-
-
6
-
-
-
-
-
-
-
-
10
10
735530
Yao
Metabolic engineering to impro ...
Thermotoga maritima
Appl. Microbiol. Biotechnol.
88
199-208
2010
-
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
695386
Goncalves
Crystallization and preliminar ...
Salmonella enterica subsp. enterica serovar Typhimurium
Acta Crystallogr. Sect. F
65
698-701
2009
-
-
1
1
-
-
-
-
-
-
2
-
-
6
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
2
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
2
-
-
-
-
-
-
695703
Dominguez
Sequential Injection Kinetic F ...
Cellulomonas sp.
Appl. Biochem. Biotechnol.
160
1664-1673
2009
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
1
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
695835
Hatayama
Regulation of glycerol metabol ...
Enterobacter aerogenes, Enterobacter aerogenes NBRC12010
Appl. Microbiol. Biotechnol.
83
749-756
2009
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
1
-
-
-
-
-
-
-
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-
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-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
696785
Zhao
Effects of over-expression of ...
Klebsiella pneumoniae, Klebsiella pneumoniae KG1
Bioprocess Biosyst. Eng.
32
313-320
2009
-
-
1
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
2
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
696879
Durnin
Understanding and harnessing t ...
Escherichia coli
Biotechnol. Bioeng.
103
148-161
2009
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
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-
-
-
1
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1
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1
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1
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-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
696915
Mouri
Design of a cytochrome P450BM3 ...
Escherichia coli, Escherichia coli JM109
Biotechnol. Prog.
25
1372-1378
2009
-
-
1
-
-
-
-
-
-
-
1
-
-
30
-
-
1
-
-
-
1
-
4
1
-
-
-
-
1
1
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
1
-
-
-
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1
-
-
1
-
4
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
699149
Zhao
-
Over-expression of glycerol de ...
Klebsiella pneumoniae, Klebsiella pneumoniae KG1
J. Chem. Technol. Biotechnol.
84
626-632
2009
-
-
1
-
-
-
-
-
-
-
1
-
-
2
-
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-
-
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2
1
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-
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1
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1
1
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1
-
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-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
699973
Zhang
Tentative identification of gl ...
Escherichia coli
Med. Microbiol. Immunol.
198
195-204
2009
-
-
1
-
-
-
-
-
-
-
-
-
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4
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
684422
Gamella
Integrated multienzyme electro ...
Cellulomonas sp.
Anal. Chim. Acta
609
201-209
2008
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
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1
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-
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-
-
-
-
-
-
-
-
-
-
-
-
699986
Gonzalez
A new model for the anaerobic ...
Escherichia coli K-12
Metab. Eng.
10
234-245
2008
-
-
1
-
-
-
-
-
-
-
-
-
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4
-
-
-
-
-
-
-
-
1
-
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-
-
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-
1
-
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1
1
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-
1
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-
-
-
-
-
-
-
-
-
-
699987
Shams Yazdani
Engineering Escherichia coli f ...
Escherichia coli K-12
Metab. Eng.
10
340-351
2008
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684616
Gaetgens
Biotransformation of glycerol ...
Gluconobacter oxydans
Appl. Microbiol. Biotechnol.
76
553-559
2007
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671323
Katrlik
-
Amperometric biosensors based ...
Cellulomonas sp.
Anal. Chim. Acta
566
11-18
2006
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1
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674122
Oliveira
Kinetic enzymatic determinatio ...
Cellulomonas sp., Enterobacter aerogenes
J. Agric. Food Chem.
54
4136-4140
2006
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2
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1
1
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1
1
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674272
Nakayama
Mechanism of hilA repression b ...
Salmonella enterica
J. Bacteriol.
188
3121-3125
2006
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675794
Lee
Application of sequential inte ...
Escherichia coli
Metab. Eng.
8
58-65
2006
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1
1
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668061
Ichinose
Enzymatic redox cofactor regen ...
Escherichia coli, Escherichia coli JM109
Biotechnol. Prog.
21
1192-1197
2005
3
1
1
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1
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2
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29
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1
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4
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1
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1
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655371
Stibor
-
Characterization of cold-activ ...
Arthrobacter sp.
Enzyme Microb. Technol.
32
532-538
2003
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1
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4
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1
1
1
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1
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657315
Brinen
Crystal structure of a zinc-co ...
Thermotoga maritima, Thermotoga maritima TM0423
Proteins
50
371-374
2003
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1
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1
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654038
Yamada-Onodera
-
Characterisation of glycerol d ...
Ogataea angusta, Ogataea angusta DL-1
Acta Biotechnol.
22
337-353
2002
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6
2
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1
2
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4
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1
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10
1
1
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6
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10
1
1
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1
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654039
Yamada-Onodera
-
Expression of the gene of glyc ...
Ogataea angusta, Ogataea angusta DL-1
Acta Biotechnol.
22
355-362
2002
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1
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4
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8
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654068
Srinivasan
Towards the crystal structure ...
Thermotoga maritima
Acta Crystallogr. Sect. D
58
867-869
2002
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656383
Pandey
Chemical modification of speci ...
Enterobacter aerogenes
J. Enzyme Inhib. Med. Chem.
17
49-53
2002
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2
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2
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636297
Malaoui
Separation and characterizatio ...
Clostridium butyricum, Clostridium butyricum E5
J. Appl. Microbiol.
90
1006-1014
2001
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-
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8
1
3
1
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4
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1
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1
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11
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4
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8
1
3
1
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1
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1
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11
-
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4
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636300
Liyanage
Clostridium beijerinckii and C ...
Clostridioides difficile, Clostridium beijerinckii
Appl. Environ. Microbiol.
67
2004-2010
2001
-
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2
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6
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636301
Burke
Purification, crystallisation ...
Geobacillus stearothermophilus
Acta Crystallogr. Sect. D
57
165-167
2001
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1
1
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1
1
1
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137617
Altaras
Metabolic engineering of a 1,2 ...
Escherichia coli
Appl. Environ. Microbiol.
65
1180-1185
1999
-
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1
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4
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636298
Leichus
Isotopic analysis of the react ...
Cellulomonas sp.
Biochemistry
33
14642-14649
1994
-
-
-
-
-
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2
8
1
1
2
1
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1
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1
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9
1
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2
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2
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2
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8
1
1
2
1
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1
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9
1
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636296
Nishise
-
Effects of organic solvents an ...
Cellulomonas sp., Cellulomonas sp. NT3060
Agric. Biol. Chem.
51
3347-3353
1987
-
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-
-
-
25
3
1
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2
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4
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1
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6
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2
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2
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25
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3
1
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2
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1
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6
-
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587013
Kong
-
Glycerol oxidation and triose ...
Schizosaccharomyces pombe, Schizosaccharomyces pombe 972h-
J. Gen. Microbiol.
131
1571-1579
1985
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1
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2
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2
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1
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1
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2
-
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1
-
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-
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636283
Marshall
-
Purification and properties of ...
Schizosaccharomyces pombe
J. Gen. Microbiol.
131
1581-1588
1985
4
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7
4
1
4
2
1
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1
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1
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1
2
9
1
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2
2
-
2
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4
-
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2
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7
-
4
1
4
2
1
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1
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1
2
9
1
-
-
-
-
2
2
-
-
-
-
-
-
-
-
636285
Gärtner
-
Purification and properties of ...
Pichia membranifaciens, Pichia membranifaciens H 112
J. Gen. Microbiol.
130
3225-3233
1984
3
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-
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11
4
1
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2
2
-
2
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1
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-
1
1
6
1
1
-
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-
3
-
1
2
2
-
4
3
-
-
2
-
-
-
4
11
2
4
1
-
2
2
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1
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-
1
1
6
1
1
-
-
-
3
-
1
-
-
-
-
-
-
-
636286
Nishise
-
Further characterization of gl ...
Cellulomonas sp., Cellulomonas sp. NT3060
Agric. Biol. Chem.
48
1603-1609
1984
1
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1
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3
2
1
1
1
2
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4
-
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1
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1
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8
1
2
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4
-
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2
2
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1
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2
1
-
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3
2
2
1
1
1
2
-
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1
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1
-
8
1
2
-
-
-
4
-
-
-
-
-
-
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-
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636287
Scharschmidt
-
Isolierung und Charakterisieru ...
Bacillus megaterium
Hoppe-Seyler's Z. Physiol. Chem.
364
911-921
1983
-
-
-
-
-
-
8
2
1
1
2
1
-
1
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1
-
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1
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3
1
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-
1
-
1
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2
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2
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8
-
2
1
1
2
1
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1
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1
-
3
1
-
-
1
-
1
-
-
-
-
-
-
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-
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636288
Sasaki
Specific affinity of glycerol ...
Geotrichum candidum
J. Biochem.
91
211-217
1982
-
-
-
-
-
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4
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1
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2
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1
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1
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4
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1
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1
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1
-
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
636289
Yamada H.; Nagao
-
Glycerol dehydrogenase from Ce ...
Cellulomonas sp., Cellulomonas sp. NT3060
Agric. Biol. Chem.
46
2333-2339
1982
1
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-
-
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9
3
1
4
-
2
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4
-
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1
-
-
-
1
-
17
-
1
-
2
-
2
2
1
2
-
-
-
1
-
-
2
-
-
-
-
9
-
3
1
4
-
2
-
-
-
1
-
-
1
-
17
-
1
-
2
-
2
2
1
-
-
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-
-
-
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636290
Yamada
-
Crystallisation of glycerol de ...
Cellulomonas sp.
Agric. Biol. Chem.
44
471-472
1980
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1
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1
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1
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1
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1
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1
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1
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1
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1
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1
-
1
-
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1
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-
-
-
-
1
-
-
-
-
-
-
-
-
-
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-
636291
Ruch
In vivo inactivation of glycer ...
Enterobacter aerogenes 2103, Enterobacter aerogenes
J. Bacteriol.
141
1077-1085
1980
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-
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1
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2
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1
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1
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2
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1
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2
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1
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1
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2
-
-
-
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-
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636292
McGregor
Purification and kinetic chara ...
Enterobacter aerogenes 1033, Enterobacter aerogenes
J. Biol. Chem.
249
3132-3139
1974
5
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6
1
1
3
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3
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1
-
-
-
1
-
2
-
-
-
-
-
-
-
-
-
-
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5
-
-
-
-
-
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6
-
1
1
3
-
-
-
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-
1
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-
1
-
2
-
-
-
-
-
-
-
-
-
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-
-
-
636293
Lin
The activation of glycerol deh ...
Enterobacter aerogenes, Enterobacter aerogenes P14
J. Biol. Chem.
235
1820-1823
1960
1
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5
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1
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3
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1
-
-
-
1
-
10
-
-
-
-
-
1
1
-
2
-
-
-
1
-
-
2
-
-
-
-
5
-
-
1
-
-
2
-
-
-
1
-
-
1
-
10
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1
1
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636294
Burton
-
Glycerol dehydrogenase from Ae ...
Enterobacter aerogenes
Methods Enzymol.
1
397-400
1955
1
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4
1
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1
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1
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1
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1
1
17
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1
3
2
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1
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2
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4
1
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1
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1
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1
1
17
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1
3
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636295
Asnis
A glycerol dehydrogenase from ...
Escherichia coli, Escherichia coli ECFS
J. Biol. Chem.
203
153-159
1953
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4
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1
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3
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1
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1
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1
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1
1
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4
-
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1
-
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1
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1
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1
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1
1
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