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Literature summary for 1.1.1.6 extracted from

  • Ichinose, H.; Kamiya, N.; Goto, M.
    Enzymatic redox cofactor regeneration in organic media: functionalization and application of glycerol dehydrogenase and soluble transhydrogenase in reverse micelles (2005), Biotechnol. Prog., 21, 1192-1197.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
ammonium sulfate about 100% activation at 10 mM Escherichia coli
K+ activates NADH production Escherichia coli
NH4+ activates NADH production Escherichia coli

Application

Application Comment Organism
molecular biology enzymatic redox cofactor regeneration in organic media: functionalization and application of recombinant glycerol dehydrogenase and soluble transhydrogenase in reverse micelles, overview Escherichia coli

Cloned(Commentary)

Cloned (Comment) Organism
expression as His-tag fusion protein in Escherichia coli BL21 (DE3) pLysS Escherichia coli
gene gld, expression of His-tagged enzyme in strain BL21(DE3) Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
NH4+ 80% activation at 10 mM Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
glycerol + NAD+ Escherichia coli
-
D-glyceraldehyde + NADH + H+
-
r
glycerol + NAD+ Escherichia coli JM109
-
D-glyceraldehyde + NADH + H+
-
r

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Escherichia coli
-
gene gld
-
Escherichia coli JM109
-
gene gld
-

Purification (Commentary)

Purification (Comment) Organism
purified by Ni-affinity chromatography Escherichia coli
recombinant His-tagged enzyme from strain BL21(DE3) by nickel affinity chromatography Escherichia coli

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
Escherichia coli
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
assay development for NADH production by recombinant enzyme in reverse micelles, micelle size optimization, overview Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glycerol + NAD+ the reaction is performed in reverse micelles harboring glycerol and NAD+ in a solution of isooctane containing 250 mM diocytlsulfosuccinate Escherichia coli dihydroxyacetone + NADH + H+
-
?
glycerol + NAD+ the reaction is performed in reverse micelles harboring glycerol and NAD+ in a solution of isooctane containing 250 mM diocytlsulfosuccinate Escherichia coli JM109 dihydroxyacetone + NADH + H+
-
?
glycerol + NAD+
-
Escherichia coli D-glyceraldehyde + NADH + H+
-
r
glycerol + NAD+
-
Escherichia coli JM109 D-glyceraldehyde + NADH + H+
-
r

Synonyms

Synonyms Comment Organism
GLD
-
Escherichia coli
glycerol dehydrogenase
-
Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at, in reverse micelles Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.5
-
in an aqueous buffer system Escherichia coli
10.5
-
in reverse micelles Escherichia coli
10.5 11 NADH production Escherichia coli

pH Range

pH Minimum pH Maximum Comment Organism
6 11
-
Escherichia coli

Cofactor

Cofactor Comment Organism Structure
additional information no activity with NADP+ and NADPH Escherichia coli
NAD+
-
Escherichia coli
NADH
-
Escherichia coli