1.1.1.6: glycerol dehydrogenase
This is an abbreviated version!
For detailed information about glycerol dehydrogenase, go to the full flat file.
Reaction
Synonyms
B4100_2156, CglD, dehydrogenase, glycerol, GDH, GDH2, GLD, Gld3, GldA, GLDH
ECTree
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Crystallization
Crystallization on EC 1.1.1.6 - glycerol dehydrogenase
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sitting-drop vapor-diffusion method at 22°C, solved at 2.8 A resolution. Each GldA monomer consists of nine beta-strands, thirteen alpha-helices, two 3(10)-helices and several loops organized into two domains, the N- and C-terminal domains. The active site is located in a deep cleft between the two domains
structure containing glycerol in the active site, to 2.8 A resolution. Each GldA monomer consists of nine beta-strands, thirteen alpha-helices, two 310-helices and several loops organized into the N- and C-terminal domains. The active site is located in a deep cleft between the two domains. The N-terminal domain contains a classic Rossmann fold for NAD+ binding. The glycerol molecule is sandwiched by the Zn2+ and NAD+ ions
crystal obtained by hanging-drop vaour-diffusion method, S305C mutant
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crystallization as a contaminant, crystal was initially assumed to be of a mutant of the SurE protein as it shares some structural features with the presumed target protein
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preliminary X-ray characterization
sitting drop vapor-diffusion method
hanging drop vapour diffusion, mixing of 0.001 ml 6 mg/ml protein in 150 mM ammonium acetate, 50 mM sodium chloride, 20 mM Tris, pH 7.4, 5% glycerol with 0.001 ml reservoir solution containing 5-10% PEG 3350, 0.2 M calcium acetate, and 4% glycerol, X-ray diffraction structure determination and analysis at 1.90 A resolution, molecular replacement using PDB entry 1jpu as a search model
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