1.1.1.6: glycerol dehydrogenase

This is an abbreviated version, for detailed information about glycerol dehydrogenase, go to the full flat file.

Reaction

glycerol
+
NAD+
=
Glycerone
+
NADH
+
H+

Synonyms

CglD, dehydrogenase, glycerol, GDH, GDH2, GLD, GldA, GLDH , GlhA, glycerin dehydrogenase, glycerol dehydrogenase, glycerol NAD 2-oxidoreductase, glycerol:NAD+ 2-oxidoreductase, GlyDH, Gro dehydrogenase, GroDHase, gycerol dehydrogenase, NAD+-dependent glycerol dehydrogenase, NAD-linked glycerol dehydrogenase, NAD-specific glycerol dehydrogenase, TmGlyDH

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.6 glycerol dehydrogenase

Cloned

Cloned on EC 1.1.1.6 - glycerol dehydrogenase

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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
CglD, GST-tagged protein, expressed in E. coli BL21 (DE3)
-
cloning from contaminating bacteria, DNA and amino acid sequence determmination and analysis, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli train BL21(DE3)pLysS
-
coexpression of enzymes recombinant glycerol dehydrogenase from Escherichia coli, lactate dehydrogenase form Bacillus subtilis, and NADH oxidase LpNox1 from Lactobacillus pentosus in Escherichia coli strain BL21(DE3). The activities of LDH, LpNox1 and GlyDH in the cell-free extract are 98.8, 27.8 and 39.5 U/ml, respectively, overview
-
expressed in E. coli
-
expressed in Klebsiella pneumoniae KG1
expressed in Klebsiella pneumoniae KG1; overexpressed in Klebsiella pneumoniae KG1
expression as His-tag fusion protein in Escherichia coli BL21 (DE3) pLysS; gene gld, expression of His-tagged enzyme in strain BL21(DE3)
-
expression in Saccharomyces cerevisiae
-
gene gldA from Escherichia coli strain JM109, expression of His-tagged enzyme in Escherichia coli strain BL21 (DE3), subcloning in Escherichia coli TOP10 cells
-
gene gldA, DNA and amino acid sequence determination and analysis, recombinant expression in Escherichia coli strain BL21(DE3)
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gene gldA, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
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gene gldA, recombinant expression in Escherichia coli strain BL21(DE3); gene gldA, recombinant expression in Escherichia coli strain BL21(DE3)
-
gene gldA, recombinant expression of C-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3)
-
His-tag, expressed in E. coli JM109; His-tagged version
-
His-tagged version expressed in Escherichia coli BL21 STAR (DE3); N-terminal His-tag, expressed in E. coli BL21 STAR (DE3)
His-tagged version expressed in Escherichia coli BL21(DE3)
overexpressed in Schizosaccharomyces pombe
recombinant co-overexpression of enzymes glycerol dehydrogenase, malate dehydrogenase, and fumarate hydratase from Klebsiella pneumoniae subsp. pneumoniae strain ATCC 12657 in Propionibacterium jensenii leads to increased increased propionic acid production, quantittive reverse transcription PCR expression analysis
-
recombinant expression of the His-tagged enzyme encoding gene fused to the codon-optimized NADH oxidase gene nox from Lactobacillus brevis, chimeric enzyme GDH-NOX, in Escherichia coli strain BL21(DE3) resulting in bioconversion of glycerol to dihydroxyacetone in the cells
-
recombinent expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) pET-32a
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