1.1.1.38: malate dehydrogenase (oxaloacetate-decarboxylating)

This is an abbreviated version, for detailed information about malate dehydrogenase (oxaloacetate-decarboxylating), go to the full flat file.

Reaction

(S)-malate
+
NAD+
=
pyruvate
+
CO2
+
NADH

Synonyms

malic enzyme, MHD, mitochondrial malic enzyme ME2, mitochondrial NAD(P) malic enzyme ME2, mitochondrial NAD-malic enzyme, NAD+-dependent malic enzyme, NAD-dependent ME, NAD-malic enzyme, NAD-ME, NAD-specific malic enzyme, pyruvic-malic carboxylase

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.38 malate dehydrogenase (oxaloacetate-decarboxylating)

Purification

Purification on EC 1.1.1.38 - malate dehydrogenase (oxaloacetate-decarboxylating)

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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
38-40c treatment, ammonium sulfate, protamine sulfate precipitation, calcium phosphate gel, ammonium sulfate
-
ammonium sulfate, DEAE-cellulose, Blue-B agarose, Orange-A agarose
-
DEAE Sepharose CL-6B column chromatography, and Q Sepharose column chromatography
-
MnCl2 precipitation, protaminsulfate precipitation, Sephadex G-25, hydroxylapatite
-
MnCl2 precipitation, protaminsulfate precipitation, Sephadex G-25, Sephadex G-200, hydroxylapatite
-
protamine sulfate precipitation, ammonium sulfate precipitation, DEAE-cellulose
-
recombinant His6-tagged enzyme from strain BL21(DE3) by nickel affinity chromatography and ultrafiltration to 95% purity
-
streptomycin sulfate ammonium sulfate, Phenyl-sepharose, Orange-A-Sepharose
-
streptomycin, ammonium sulfate, heat treatment, acid treatment, calcium phosphate gel, DEAE-sephadex, hydroxylapatite, Sepharose 6B
-