1.1.1.38: malate dehydrogenase (oxaloacetate-decarboxylating)

This is an abbreviated version, for detailed information about malate dehydrogenase (oxaloacetate-decarboxylating), go to the full flat file.

Reaction

(S)-malate
+
NAD+
=
pyruvate
+
CO2
+
NADH

Synonyms

malic enzyme, MHD, mitochondrial malic enzyme ME2, mitochondrial NAD(P) malic enzyme ME2, mitochondrial NAD-malic enzyme, NAD+-dependent malic enzyme, NAD-dependent ME, NAD-malic enzyme, NAD-ME, NAD-specific malic enzyme, pyruvic-malic carboxylase

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.38 malate dehydrogenase (oxaloacetate-decarboxylating)

Inhibitors

Inhibitors on EC 1.1.1.38 - malate dehydrogenase (oxaloacetate-decarboxylating)

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,10-phenanthroline
-
0.025 mM, 70% activity lost after 5 min
1-(5-O-phosphono-beta-D-ribofuranosyl)-4-[2-(trifluoromethyl)phenyl]-1H-1,2,3-triazole
-
2-methylbutanoate
-
5.22 mM, competitive inhibitor, 60% inhibition at pH 7.5, 54% inhibition at pH 7.0
2-methylpentanoate
-
5.22 mM, competitive inhibitor, 45% inhibition at pH 7.5, 40% inhibition at pH 7.0
4,4'-dithiodipyridine
-
sulfhydryl reagent, rapid and complete loss of activity, presence of Mg2+ or Mn2+ enhances inactivation rate
4-(2-aminophenyl)-1-(5-O-phosphono-beta-D-ribofuranosyl)-1H-1,2,3-triazole
-
4-(2-fluorophenyl)-1-(5-O-phosphono-beta-D-ribofuranosyl)-1H-1,2,3-triazole
-
4-(2-hydroxyethyl)-1-(5-O-phosphono-beta-D-ribofuranosyl)-1H-1,2,3-triazole
-
4-(2-methylphenyl)-1-(5-O-phosphono-beta-D-ribofuranosyl)-1H-1,2,3-triazole
-
4-(4-fluorophenyl)-1-(5-O-phosphono-beta-D-ribofuranosyl)-1H-1,2,3-triazole
-
4-(4-methoxyphenyl)-1-(5-O-phosphono-beta-D-ribofuranosyl)-1H-1,2,3-triazole
-
4-(naphthalen-1-yl)-1-(5-O-phosphono-beta-D-ribofuranosyl)-1H-1,2,3-triazole
-
4-phenyl-1-(5-O-phosphono-beta-D-ribofuranosyl)-1H-1,2,3-triazole
-
5,5'-dithiobis(2-nitrobenzoic acid)
acetyl-CoA
AgNO3
-
inhibition with 20 mM, complete inhibition with 300 mM
Cl-
-
competitive vs. malate and NAD+
D-malate
-
competitive inhibition
diethyldicarbonate
-
80% inactivation after treatment with 5 mM diethyldicarbonate for 25 min, biphasic inactivation, 40-50% inactivation in first phase, 1-2 histidine residues are acylated by diethyldicarbonate, 250 mM malate provides complete protection, 50% protection with 50 mM MgSO4, 55% enzyme activity is restored with 0.5 M hydroxylamine
iodoacetate
malonate
-
competitive inhibition
N-acetyl-1-(5-O-phosphono-beta-D-ribofuranosyl)-1H-1,2,3-triazol-4-amine
-
N-benzoyl-1-(5-O-phosphono-beta-D-ribofuranosyl)-1H-1,2,3-triazol-4-amine
-
N-ethylmaleimide
oxaloacetate
p-(chloromercuri)benzoate
succinate
Tartronate
-
competitive inhibition vs. malate, noncompetitve vs. NAD+
tiglic acid
-
5.22 mM, competitive inhibitor, 58% inhibition at pH 7.5, 52% inhibition at pH 7.0
Zn2+
-
5 mM ZnCl2
additional information
-